Results 71 to 80 of about 388,328 (197)

An isothermal amplification method for personal diagnosis

, 2018
funder : 과학기술정보통신부funder : Ministry of Science and ICT개인이 가정에서 진단을 수행할 수 있는 등온증폭 기술의 개발을 위해서 본 연구에서는 기존의 루프매개 등온증폭법(Loop-mediated isothermal amplification assay, 이하 LAMP)의 한계점인 등온 증폭용 프라이머의 성능 저하 등 문제점을 해결하기 위해서 CRISPER-CAS9을 기반으로 하는 새로운 등온증폭 방법을 제안하였다 ...

core   +1 more source

Isothermal amplification using a chemical heating device for point-of-care detection of HIV-1.

PLoS ONE, 2012
BackgroundTo date, the use of traditional nucleic acid amplification tests (NAAT) for detection of HIV-1 DNA or RNA has been restricted to laboratory settings due to time, equipment, and technical expertise requirements.
Kelly A Curtis, Donna L Rudolph, Irene Nejad, Jered Singleton, Andy Beddoe, Bernhard Weigl, Paul LaBarre, S Michele Owen   +7 more
doaj   +1 more source

Quality Control of Isothermal Amplified DNA Based on Short Tandem Repeat Analysis

, 2015
This protocol describes the use of a 16plex PCR for the purpose assessing DNA quality after isothermal whole genome amplification (WGA). In short, DNA products, generated by amplification multiple displacement amplification, are forwarded to PCR ...
Amin El-Heliebi, Thomas Kroneis, El-Heliebi, A., Kroneis, Thomas,   +3 more
core   +1 more source

Simultaneous detection of multiple benzimidazole resistant β-tubulin variants of Botrytis cinerea using loop mediated isothermal amplification [PDF]

, 2018
Optimal disease management depends on the ability to monitor the development of fungicide resistance in plant pathogen populations. Benzimidazole resistance is caused by the point mutations of the β-tubulin gene in Botrytis cinerea, and three mutations (
Gero Steinberg, Ya Bing Duan, Chang Jun Chen, Zhou, M. G., Jian Xin Wang, Yang, Y., Steinberg, G., Chen, C. J., Fraaije, B. A., Duan, Y., Ming Guo Zhou, Wang, J. X., Ying Yang, Bart A Fraaije   +13 more
core   +1 more source

Onsite detection of plant viruses using isothermal amplification assays

, 2022
Plant diseases caused by viruses limit crop production and quality, resulting in significant losses. However, options for managing viruses are limited; for example, as systemic obligate parasites, they cannot be killed by chemicals.
Magdy Mahfouz, Mahfouz, Magdy M., Alangar I. Bhat, Bhat, A I, Bhat, Alangar I., Aman, Rashid, Rashid Aman, Mahfouz, Magdy   +7 more
core   +1 more source

Isothermal cross-priming amplification for field-deployable norovirus surveillance: a cost-effective and rapid solution

Microbiology Spectrum
Norovirus remains a leading cause of foodborne gastroenteritis globally. While isothermal amplification technologies offer advantages over traditional reverse transcription quantitative PCR (RT-qPCR) in terms of speed and portability, there is a lack of ...
Ran Jia, Lijuan Lu, Xunhua Zhu, Lingfeng Cao, Liyun Su, Huaqing Zhong, Menghua Xu, Pengcheng Liu, Yanqiong Zhou, Jin Xu   +9 more
doaj   +1 more source

Pathogenic Bacteria Detection Using RNA-Based Loop-Mediated Isothermal-Amplification-Assisted Nucleic Acid Amplification via Droplet Microfluidics

, 2019
Nucleic acid amplifications, such as polymerase chain reaction (PCR), are very beneficial for diagnostic applications, especially in the context of bacterial or viral outbreaks due to their high specificity and sensitivity.
Alireza Abbaspourrad (1305903), Meisam Zaferani (6001046), Soon Hon Cheong (537860), Morteza Azizi (4915273)   +3 more
core   +1 more source

Determining putative vectors of the Bogia Coconut Syndrome Phytoplasma using loop-mediated isothermal amplification of single insect-feeding media [PDF]

, 2016
Phytoplasmas are insect vectored mollicutes responsible for disease in many economically important crops. Determining which insect species are vectors of a given phytoplasma is important for managing disease but is methodologically challenging because ...
Lu, Hengyu, Ash, Gavin J., Fletcher, Murray J., You, Minsheng, Yang, Guang, Gurr, Geoff M., Woruba, Sharon B., Wilson, Bree A. L.   +7 more
core   +1 more source

Proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection

, 2017
Yanyan Yu, Gaoxing Su, Hongyan Zhu, Qing Zhu, Yong Chen, Bohui Xu, Yuqin Li, Wei Zhang School of Pharmacy, Nantong University, Nantong, People’s Republic of China Abstract: In this study, we fabricated a novel electrochemical biosensing platform ...
Zhang W, Li Y, Xu B, Zhu H, Chen Y, Zhu Q, Su G, Yu Y   +7 more
core  

Loop-mediated isothermal amplification and its limit of detection for diagnostics of plant pathogens

Phytopathology deals with a branch of biology encompassing pathogens that infect plants. Pathogenic fungi, bacteria, viruses, viroids, and phytoplasmas are notorious and hard to control; preventive measures are important for managing disease as early as ...
Govindan Muthukumar, Shanmugam Varanavasiappan, Pachamuthu Kamaraj, Irudhayasamy Johnson, Iyyamperumal Muthuvel, Ayyanar Kamalakannan   +5 more
core   +1 more source