Results 81 to 90 of about 388,328 (197)

Isothermal DNA amplification in bioanalysis: strategies and applications

, 2011
Isothermal DNA amplification is an alternative to PCR-based amplification for point-of-care diagnosis. Since the early 1990s, the approach has been refined into a simple, rapid and cost-effective tool by means of several distinct strategies.
Joonyul Kim, Christopher J Easley
core   +1 more source

One-step loop-mediated isothermal amplification system for Mycobacterium marinum detection

Microbiology Spectrum
Mycobacterium marinum is the etiological agent of cutaneous nontuberculous mycobacteriosis, inducing granuloma formation in human skin. Other mycobacterial species, including Mycobacterium ulcerans, Mycobacterium chelonae, and Mycobacterium fortuitum ...
Kayo Okumura, Yuji Miyamoto, Satoshi Mitarai, Manabu Ato   +3 more
doaj   +1 more source

Nucleic acid isothermal amplification technologies - A review

, 2008
Nucleic acid amplification technologies are used in the field of molecular biology and recombinant DNA technologies. These techniques are used as leading methods in detecting and analyzing a small quantity of nucleic acids. The polymerase chain reaction (
Gill, P., Ghaemi, A.
core  

Low-Volume On-Chip Single-Cell Whole Genome Amplification for Multiple Subsequent Analyses

, 2015
Multiple analyses such as DNA profiling, sequencing, or comparative genome hybridization (CGH) done on the single-cell level long for pre-amplification due to the diploid human genome.
Amin El-Heliebi, Thomas Kroneis, El-Heliebi, A., Shukun Chen, Kroneis, Thomas,, Chen, S.   +5 more
core   +1 more source

Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board

PLoS ONE, 2023
Recent advances in electronics and microfluidics have enabled several research groups to develop fully integrated, sample-to-result isothermal nucleic acid amplification test (NAAT) platforms for the point of care.
Kamal G. Shah, Mike Roller, Sujatha Kumar, Steven Bennett, Erin Heiniger, Katriel Looney, Joshua Buser, Joshua D. Bishop, Paul Yager   +8 more
doaj  

Enhancing Biosecurity in Mollusc Aquaculture: A Review of Current Isothermal Nucleic Acid Detection Methods

Animals
The growing human population has increased the need for food beyond what terrestrial sources can provide. This boosts aquaculture demand for molluscs, fish, and crustaceans.
Hoda Abbas, Gemma Zerna, Alexandra Knox, Danielle Ackerly, Jacinta Agius, Karla Helbig, Travis Beddoe   +6 more
doaj   +1 more source

Establishment and application of isothermal multiple-self-matching-initiated amplification (IMSA) in detecting Type II heat-labile enterotoxin of Escherichia coli.

PLoS ONE, 2019
Enterotoxigenic Escherichia coli (ETEC) constitutes a major cause of diarrhea in young children and animals, particularly in poor regions of the world, as well the traveler's diarrhea in adult individuals.
Wenxin Liu, Chaowen Yuan, Liguo Zhang, Yufei Feng   +3 more
doaj   +1 more source

Quantitative comparison of isothermal DNA amplification methods

, 2013
In the field of isothermal DNA amplification, four of the widest used methods are recombinase polymerase amplification (RPA), helicase-dependent amplification (HDA), isothermal and chimeric primer initiated amplification of nucleic acids (ICAN) and loop ...
Wu, Yuefeng (Rose)
core   +1 more source

Establishment of Isothermal Rapid Detection Method for Salmon-derived Components in Food

Shipin gongye ke-ji
This article presented a visualization method for detecting salmon-derived components in Oncorhynchus mykiss and Salmo salar, based on fluorescence-labeled loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA).
Bo PENG, Lin HONG, Shiguang GAO, Wenchao CHEN, Xin ZHAO, Yanhong LIU, Qiuyue ZHENG   +6 more
doaj   +1 more source

Annexing isothermal nucleotide amplification enables probe-guided amplification and direct detection at ambient temperature

Nature Communications
Isothermal nucleic acid amplification offers advantages over qPCR for decentralized diagnostics but remains constrained by rigid primer design, detection complexity, and equipment dependence.
Ariana Human-McKinnon, Madisen Pyper, MiKenzee Frazier, Emily Plant, Harrison Piper, Tyler Archibald, Anindita Roy   +6 more
doaj   +1 more source