Results 81 to 90 of about 63,752 (305)
Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes
BioTechniques, 2015 Nucleic acid amplification is the basis for many molecular diagnostic assays. In these cases, the amplification product must be detected and analyzed, typically requiring extended workflow time, sophisticated equipment, or both.
Nathan A. Tanner +2 more
doaj +1 more source
Nickase-dependent isothermal DNA amplification
Advances in Bioscience and Biotechnology, 2013 We developed a nicking endonuclease dependent DNA amplification (NDA), using Nt.BstNBI to catalyze single-stranded nick on double-stranded DNA, and Bst DNA polymerase to make extension while sealing the nick and displacing the downstream strand. The displaced single-stranded DNA thereby serves as template for primers hybridization and extension ...
Yan He, Tao Jiang
openaire +2 more sources
Turbulence and Dynamo Interlinks
, 2012 The role of turbulence in astrophysical environments and its interplay with magnetic fields is still highly debated. In this lecture, we will discuss this issue in the framework of dynamo processes. We will first present a very brief summary of turbulent
Falceta-Gonçalves, D. +3 more
core +1 more source
Advanced Healthcare Materials, EarlyView.Engineered microparticle topographies direct human mesenchymal stem cell osteogenesis without biochemical additives. This osteogenic commitment is driven by canonical Hedgehog signaling and followed by temporal IGF‐II engagement. Two‐photon polymerization demonstrates spatial control, enabling the engineering of topographical gradients that pattern ...
Fatmah I. Ghuloum +5 more
wiley +1 more source
Bacteria‐Responsive Nanostructured Drug Delivery Systems for Targeted Antimicrobial Therapy
Advanced Materials, EarlyView.Bacteria‐responsive nanocarriers are designed to release antimicrobials only in the presence of infection‐specific cues. This selective activation ensures drug release precisely at the site of infection, avoiding premature or indiscriminate release, and enhancing efficacy.
Guillermo Landa +3 more
wiley +1 more source
BioTechniquesStrand displacement amplification (SDA) is an isothermal amplification technique wherein amplification of a nucleic acid is initiated by nicking enzyme activity at sites flanking the target.
Yinhua Zhang +2 more
doaj +1 more source
Advanced Materials, EarlyView.A cryoprotectant‐compatible nanoporous platform enables ambient‐stable and scalable delivery of gene editing therapeutics. By combining hierarchical pore architecture with optimized lyophilization chemistry, the system preserves Cas9‐RNP activity post‐freeze‐drying.
Sian Lee +8 more
wiley +1 more source
环境与职业医学Water resources are crucial for environmental protection and the health of humans, plants, and animals. Contamination of water by pathogens such as bacteria, viruses, and protozoa can lead to outbreaks of various water-related infectious diseases, posing
Fubin HUANG, Eryi SHU, Hongliang FAN
doaj +1 more source
Quasi-geometrical Optics Approximation in Gravitational Lensing
, 2004 The gravitational lensing of gravitational waves should be treated in the wave optics instead of the geometrical optics when the wave length $\lambda$ of the gravitational waves is larger than the Schwarzschild radius of the lens mass $M$.
Baraldo +15 more
core +1 more source
Polymorph‐Specific Electronic Transduction in WO3 during Molecular Sensing
Advanced Materials, EarlyView.Metal‐oxide polymorphs with similar surface chemistry can nevertheless exhibit distinct sensing properties. In γ‐ and ε‐WO3, analyte adsorption appears comparable; yet, only ε‐WO3 induces a pronounced lattice electronic perturbation that accommodates charge in sub‐conduction band minimum states.
Matteo D'Andria +6 more
wiley +1 more source