Results 61 to 70 of about 388,328 (197)

Isothermal amplification of long DNA fragments at low temperature by improved strand displacement amplification

BioTechniques
Strand displacement amplification (SDA) is an isothermal amplification technique wherein amplification of a nucleic acid is initiated by nicking enzyme activity at sites flanking the target.
Yinhua Zhang, Ashley N Luck, Nathan A Tanner   +2 more
doaj   +1 more source

Research progress on application of novel isothermal amplification technology in waterborne pathogens detection

环境与职业医学
Water resources are crucial for environmental protection and the health of humans, plants, and animals. Contamination of water by pathogens such as bacteria, viruses, and protozoa can lead to outbreaks of various water-related infectious diseases, posing
Fubin HUANG, Eryi SHU, Hongliang FAN
doaj   +1 more source

Research progress on nucleic acid amplification–based detection technologies for phytopathogenic fungi

Applied Microbiology and Biotechnology
Phytopathogenic fungi are highly diverse and globally distributed, posing a major threat to agricultural production worldwide. The annual losses caused by plant diseases can reach up to 30% of global crop yields, with over 80% of infections caused by ...
Baoyu Wang, Mei Liu, Hui Wang, Chenfang Wang, Wei Zhang, Jiye Yan   +5 more
doaj   +1 more source

Isothermal amplification-assisted diagnostics for COVID-19

, 2022
The scenery of molecular diagnostics for infectious diseases is rapidly evolving to respond to the COVID-19epidemic. The sensitivity and specificity of diagnostics, along with speed and accuracy, are crucial requirements for effective analytical tools to
Viviana Scognamiglio, Amina Antonacci, Daniele Zappi, Mariarosaria De Falco, Mariarita De Felice   +4 more
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Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection [PDF]

, 2015
© 2015 Rosser et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in ...
Rollinson, D, A. Rosser, D. Rollinson, Webster, BL, Rosser, A, Forrest, M, M. Forrest, B L Webster   +7 more
core   +1 more source

Types and Applications of Nicking Enzyme-Combined Isothermal Amplification

, 2022
Due to the sudden outbreak of COVID-19 at the end of 2019, rapid detection has become an urgent need for community clinics and hospitals. The rapid development of isothermal amplification detection technology for nucleic acids in the field of molecular ...
Xiaochen Tang, Chen, Tianshu, Cao, Siyu, Siyu Cao, Tianshu Chen, Guifang Chen, Chen, Guifang, Tang, Xiaochen   +7 more
core   +1 more source

Two Methods for Increased Specificity and Sensitivity in Loop-Mediated Isothermal Amplification

Molecules, 2015
The technique of loop-mediated isothermal amplification (LAMP) utilizes four (or six) primers targeting six (or eight) regions within a fairly small segment of a genome for amplification, with concentration higher than that used in traditional PCR ...
De-Guo Wang, Jeffrey D. Brewster, Moushumi Paul, Peggy M. Tomasula   +3 more
doaj   +1 more source

A novel cassette method for probe evaluation in the designed biochips [PDF]

, 2013
A critical step in biochip design is the selection of probes with identical hybridisation characteristics. In this article we describe a novel method for evaluating DNA hybridisation probes, allowing the fine-tuning of biochips, that uses cassettes with ...
Julian Mitchell (572357), Irina Bogdarina, Abdulmohsen A. Al-Humam (572361), Nelly Sapojnikova (572356), Bogdarina, Irina, Vitaly Zinkevich, Sapojnikova, Nelly, Mitchell, Julian, Julian Mitchell, Al-humam, Abdulmohsen A., Nino Asatiani (572359), Zinkevich, Vitaly, Samia Alkhalil (572360), Abdulmohsen A Al-Humam, Nelly Sapojnikova, Irina Bogdarina (367051), Tamar Kartvelishvili, Vitaly Zinkevich (572355), Samia Alkhalil, Kartvelishvili, Tamar, Abdulmohsen A. Al-humam, Nino Asatiani, Alkhalil, Samia, Tamar Kartvelishvili (572358), Asatiani, Nino   +24 more
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A direct multiplex isothermal amplification-reverse dot blot hybridization system for β-thalassemia diagnosis

Annals of Hematology
Molecular diagnostic methods largely rely on expensive equipment and complex operations, which makes it difficult to achieve rapid and low-cost detection.
Chao Ye, Xiaoxing Zhou, Yan Wei, Yilian Zhao, Mengru Xie, Xinchu Liu, Jinghui Ma, Jilin Qing, Zhizhong Chen   +8 more
doaj   +1 more source

Kinetic Modeling of Isothermal or Non-isothermal Adsorption in a Pellet: Application to Adsorption Heat Pumps [PDF]

, 2010
Understanding the interaction between a fluid and a solid phase is of fundamental importance to the design of an adsorption process. Because the heat effects associated with adsorption are comparatively large, the assumption of isothermal behavior is a ...
Nikov, Iordan, Montastruc, Ludovic, Ludovic Montastruc, Pascal Floquet, Iordan Nikov, Mayer, Volker, Serge Domenech, Volker Mayer, Floquet, Pascal, Domenech, Serge   +9 more
core   +1 more source