Results 31 to 40 of about 63,752 (305)

A novel cassette method for probe evaluation in the designed biochips [PDF]

, 2014
A critical step in biochip design is the selection of probes with identical hybridisation characteristics. In this article we describe a novel method for evaluating DNA hybridisation probes, allowing the fine-tuning of biochips, that uses cassettes with ...
Al-humam, Abdulmohsen A., Alkhalil, Samia, Asatiani, Nino, Bogdarina, Irina, Kartvelishvili, Tamar, Mitchell, Julian, Sapojnikova, Nelly, Zinkevich, Vitaly   +7 more
core   +4 more sources

Comparative analysis of methods for isothermal amplification of nucleic acids

Журнал микробиологии, эпидемиологии и иммунобиологии, 2022
In this review, methods for isothermal amplification of nucleic acids are considered and analyzed, in particular, loop isothermal amplification of DNA and RNA (LAMP/RT-LAMP), helicase-dependent amplification (HDA) and recombinase polymerase amplification
O. S. Chemisova, O. A. Tsyrulina, A. L. Trukhachev, A. K. Noskov   +3 more
doaj   +1 more source

Real-time gene analysis based on a portable electrochemical microfluidic system

Electrochemistry Communications, 2020
Isothermal amplification, such as loop-mediated nucleic acid isothermal amplification (LAMP), is a highly promising technique that could revolutionize portable or point-of-care gene detection.
Yichen Liu, Baiyang Lu, Yidan Tang, Yan Du, Bingling Li   +4 more
doaj   +1 more source

Evaluation of two lyophilized molecular assays to rapidly detect foot-and-mouth disease virus directly from clinical samples in field settings [PDF]

, 2017
Accurate, timely diagnosis is essential for the control, monitoring and eradication of foot‐and‐mouth disease (FMD). Clinical samples from suspect cases are normally tested at reference laboratories.
Armson, B., Chepkwony, E., Cleaveland, S., Fowler, V.L., Howson, E.L.A., Kandusi, S., Kasanga, C.J., King, D.P., Lembo, T., Madi, M., Martin, P., Mioulet, V., Sallu, R., Siddle, A., Wood, J.   +14 more
core   +1 more source

Rolling Circle and Loop Mediated Isothermal Amplification Strategy for Ultrasensitive miRNA Detection

Separations, 2021
Rolling circle amplification (RCA) and loop mediated isothermal amplification (LAMP) were combined to establish the rolling circle and loop mediated isothermal amplification (RC-LAMP) method for miRNA detection.
Zheng Cao, Xianfeng Jiang, Guizhou Xiao, Mingcheng Xu, Hui Liu, Sheng Cai   +5 more
doaj   +1 more source

Isothermal Amplification of Rabies Virus Gene

Journal of Veterinary Medical Science, 2003
A sensitive and specific in situ amplification technique is needed to elucidate the dynamics of rabies virus in the body during the long incubation period after infection. To overcome the disadvantage of using the traditional reverse transcription (RT)-PCR in in situ studies, an isothermal nucleic acid sequence-based amplification (NASBA) technique was
Makoto, Sugiyama, Naoto, Ito, Nobuyuki, Minamoto   +2 more
openaire   +3 more sources

Mapping Cluster Mass Distributions via Gravitational Lensing of Background Galaxies [PDF]

, 1994
We present a new method for measuring the projected mass distributions of galaxy clusters. The gravitational amplification is measured by comparing the joint distribution in redshift and magnitude of galaxies behind the cluster with that of field ...
Broadhurst, T. J., Peacock, J. A., Taylor, A. N.   +2 more
core   +2 more sources

Advances in Nucleic Acid Amplification-Based Microfluidic Devices for Clinical Microbial Detection

Chemosensors, 2022
Accurate and timely detection of infectious pathogens is urgently needed for disease treatment and control of possible outbreaks worldwide. Conventional methods for pathogen detection are usually time-consuming and labor-intensive.
Thi Ngoc Diep Trinh, Nae Yoon Lee
doaj   +1 more source

Isothermal cross-priming amplification implementation study

Letters in Applied Microbiology, 2015
Abstract Cross-priming amplification (CPA) was evaluated for the early detection of norovirus (NV), rotavirus A (RV-A), enteric adenovirus (EAdV) and astrovirus (AstV). The analytical sensitivity of the CPA assay was 103 copies ml−1 for NV, RV-A and AstV detection and 104 copies ml−1 for EAdV detection.
Z, Bai, H, Xie, Q, You, S, Pickerill, Y, Zhang, T, Li, J, Geng, L, Hu, H, Shan, B, Di   +9 more
openaire   +2 more sources

Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR

Practical Laboratory Medicine
Isothermal nucleic acid amplification methods have gained prominence as a valuable innovation in molecular diagnostics. These methods function at a constant temperature without the requirement of thermal cycler apparatus, simplifying the amplification ...
Alazar Amare Amdiyee, Tesfaye Sisay Tessema   +1 more
doaj   +1 more source