Results 31 to 40 of about 33,911 (295)

Loop-Mediated Isothermal Amplification (LAMP) as a Promising Point-of-Care Diagnostic Strategy in Avian Virus Research

Animals, 2021
Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that ...
Faiz Padzil, Abdul Razak Mariatulqabtiah, Wen Siang Tan, Kok Lian Ho, Nurulfiza Mat Isa, Han Yih Lau, Jalila Abu, Kuo-Pin Chuang   +7 more
doaj   +1 more source

Isothermal amplification of long DNA segments by quadruplex priming amplification [PDF]

Analytical Methods, 2018
Amplification of long DNA segments with the highest possible specificity and lowest bias is one of the main goals of modern genomics.
Levan Lomidze, Tyler H. Williford, Karin Musier-Forsyth, Besik Kankia   +3 more
openaire   +2 more sources

Cross Priming Amplification: Mechanism and Optimization for Isothermal DNA Amplification [PDF]

Scientific Reports, 2012
CPA is a class of isothermal amplification reactions that is carried out by a strand displacement DNA polymerase and does not require an initial denaturation step or the addition of a nicking enzyme. At the assay temperature of 63°C, the formation of a primer-template hybrid at transient, spontaneous denaturation bubbles in the DNA template is favored ...
Gaolian Xu, Lin Hu, Huayan Zhong, Hongying Wang, Sei-ichi Yusa, Tristen C. Weiss, Paul J. Romaniuk, Sam Pickerill, Qimin You   +8 more
openaire   +2 more sources

Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for detection of Leishmania DNA in buffy coat from visceral leishmaniasis patients [PDF]

, 2012
BACKGROUND: Visceral leishmaniasis (VL) remains as one of the most neglected tropical diseases with over 60% of the world's total VL cases occurring in the Indian subcontinent.
Mondal, D., Khondaker Rifat Hasan Bhaskar, Tania Akther, Gerd Pluschke, Bhaskar, Khondaker Rifat Hasan, Pluschke Gerd, Akther, Tania, Salam, M. A., Mondal, Dinesh, Khan, M. G., Khan Md Gulam Musawwir, Salam Md Abdus, Akther Tania, Dinesh Mondal, Pluschke, G., Md Abdus Salam, Khan, Md. Gulam Musawwir, Salam, Md. Abdus, Mondal Dinesh, Bhaskar Khondaker Rifat Hasan, Md Gulam Musawwir Khan, Akther, T., Pluschke, Gerd, Bhaskar, K. R.   +23 more
core   +1 more source

Data for designing two isothermal amplification assays for the detection of root-infecting fungi on cool-season turfgrasses

Data in Brief, 2018
Loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are two rapid isothermal amplification methods for detecting three common fungal root pathogens of cool-season turfgrass: Gaeumannomyces avenae, Magnaporthiopsis
Brijesh B. Karakkat, Kurt Hockemeyer, Margot Franchett, Megan Olson, Cortney Mullenberg, Paul L. Koch   +5 more
doaj   +1 more source

Using detergent to enhance detection sensitivity of African trypanosomes in human CSF and blood by Loop-Mediated Isothermal Amplification (LAMP) [PDF]

, 2011
<p><b>Background:</b> The loop-mediated isothermal amplification (LAMP) assay, with its advantages of simplicity, rapidity and cost effectiveness, has evolved as one of the most sensitive and specific methods for the detection of a ...
Gibson, W., Thekisoe, Oriel M M, Dumler, JS, Olga V. Nikolskaia, Olga V Nikolskaia, Liam J Morrison, Inoue, N., Noboru Inoue, Liam J. Morrison, Dennis J Grab, Oriel M. M. Thekisoe, Dumler J. Stephen, Grab, DJ, J. Stephen Dumler, Dumler, J.S., J Stephen Dumler, Morrison, L.J., Nikolskaia Olga V., Dumler, J Stephen, Nikolskaia, O.V., Nikolskaia, OV, Grab, D.J., Grab, Dennis J, Wendy Gibson, Morrison Liam J., Grab Dennis J., Inoue, N, Gibson, WC, Thekisoe, Oriel M. M., Oriel M M Thekisoe, Inoue, Noboru, Thekisoe Oriel M. M., Morrison, Liam J; id_orcid, Gibson Wendy, Thekisoe, OMM, Morrison, L, Inoue Noboru, Dennis J. Grab, Nikolskaia, Olga V, Gibson, Wendy   +39 more
core   +1 more source

Rapid Detection of Virus Nucleic Acid via Isothermal Amplification on Plasmonic Enhanced Digitizing Biosensor

Biosensors, 2022
Rapid detection for infectious diseases is highly demanded in diagnosis and infection prevention. In this work, we introduced a plasmonic enhanced digitizing biosensor for the rapid detection of nucleic acids.
Shih-Chung Wei, Chia-Chen Chang, Tsung-Liang Chuang, Kung-Bin Sung, Chii-Wann Lin   +4 more
doaj   +1 more source

Loop-mediated isothermal amplification (LAMP) for point-of-care detection of asymptomatic low-density malaria parasite carriers in Zanzibar. [PDF]

, 2015
BACKGROUND: Asymptomatic, low parasite density malaria infections are difficult to detect with currently available point-of-care diagnostics. This study piloted a loop-mediated isothermal amplification (LAMP) kit for field-friendly, high-throughput ...
Msellem, Mwinyi, Abass, Ali K, Mårtensson, Andreas, Schmidt, Berit, Abass, Ali K., Berit Aydin-Schmidt, Mwinyi Msellem, Nassor, Majda H, Cook, Jackie, Aydin-Schmidt, Berit, Nassor, Majda H., Björkman, Anders, Abdullah Ali, Elin Edlund, David Bell, Majda H Nassor, Andreas Mårtensson, Jackie Cook, Ali, Abdullah, Mårtensson, Andreas,, Anders Björkman, Gonzalez, Iveth J., González, Iveth J, Bell, David, Edlund, Elin, Ali K Abass, Iveth J González   +26 more
core   +1 more source

Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics.

PLoS ONE, 2020
A number of isothermal DNA amplification technologies claim to be ideal for point-of-need (PON) applications as they enable reactions to be performed using a single-temperature heat source (e.g. water bath).
Yiping Zou, Michael Glenn Mason, Jose Ramon Botella   +2 more
doaj   +1 more source

Ligation-free isothermal nucleic acid amplification

Biosensors and Bioelectronics, 2022
In this study, we uncover a ligation-free DNA extension method in two adjacent fragmented probes, which are hybridized to target RNA, for developing a ligation-free nucleic acid amplification reaction. In this reaction, DNA elongation occurs from a forward probe to a phosphorothioated-hairpin probe in the presence of target RNA regardless of ligation ...
Jeong Moon, Jayeon Song, Hyowon Jang, Hyunju Kang, Yong-Min Huh, Hye Young Son, Hyun Wook Rho, Mirae Park, Chandana S. Talwar, Kwang-Hyun Park, Euijeon Woo, Jaewoo Lim, Eun-Kyung Lim, Juyeon Jung, Yongwon Jung, Hyun Gyu Park, Taejoon Kang   +16 more
openaire   +3 more sources