Results 31 to 40 of about 26,794 (255)

Rolling circle amplification as isothermal gene amplification in molecular diagnostics [PDF]

BioChip Journal, 2016
Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases.
Goo, Nam-In, Kim, Dong-Eun
openaire   +2 more sources

Isothermal Amplification of Long, Discrete DNA Fragments Facilitated by Single-Stranded Binding Protein

Scientific Reports, 2017
Isothermal amplification methods for detection of DNA and RNA targets have expanded significantly in recent years, promising a new wave of simple and rapid molecular diagnostics. Current isothermal methods result in the generation of short fragments (
Yinhua Zhang, Nathan A. Tanner
doaj   +1 more source

A Sensitive Potentiometric Sensor for Isothermal Amplification-Coupled Detection of Nucleic Acids

Sensors, 2018
A disposable potentiometric sensor was newly developed for the amplification-coupled detection of nucleic acids. The hydrogen-ion is generally released during isothermal amplification of nucleic acids.
Kang-Ho Lee, Dongkyu Lee, Jongsu Yoon, Ohwon Kwon, Jaejong Lee   +4 more
doaj   +1 more source

Molecular Methods for Identification and Quantification of Foodborne Pathogens

Molecules, 2022
Foodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne ...
Min Zhang, Jiajia Wu, Zhaoai Shi, Aocheng Cao, Wensheng Fang, Dongdong Yan, Qiuxia Wang, Yuan Li   +7 more
doaj   +1 more source

Development of SARS-CoV-2 Isothermal Amplification Detection Kits

Nano Biomedicine and Engineering, 2020
The SARS-CoV-2 isothermal amplification detection kits based on loop-mediated isothermal amplification (LAMP) were developed and evaluated on three types samples of SARS-CoV-2. The kits included enzyme reaction mixtures and chromogenic agents.
Xueling Li, Jing Tian, Yan Xu, Xiaomin Chen, Zexi Liu, Hui Liang, Caihong Jin, Daxiang Cui   +7 more
doaj   +1 more source

Chip-Based and Wearable Tools for Isothermal Amplification and Electrochemical Analysis of Nucleic Acids

Chemosensors, 2022
The determination of nucleic acids has become an analytical diagnostic method with many applications in fields such as biomedical sciences, environmental monitoring, forensic identification, and food safety.
Claudia Patiti, Patrick Severin Sfragano, Serena Laschi, Serena Pillozzi, Anna Boddi, Olivia Crociani, Andrea Bernini, Ilaria Palchetti   +7 more
doaj   +1 more source

Real-time isothermal nucleic acid amplification detection in resource-limited settings: a description of an open-source miniature fluorimeter

The Lancet Global Health, 2021
Background: The detection and quantification of nucleic acids traditionally relies on PCR, which requires the use of expensive thermocyclers with integrated fluorescence detection of amplicons.
Jackson Coole, BS, Alex Kortum, MSc, Yubo Tang, PhD, Imran Vohra, BS, Mary Natoli, PhD, Kathryn Kundrod, PhD, Rebecca Richards-Kortum, PhD   +6 more
doaj   +1 more source

Rapid on-site nucleic acid testing: On-chip sample preparation, amplification, and detection, and their integration into all-in-one systems

Frontiers in Bioengineering and Biotechnology, 2023
As nucleic acid testing is playing a vital role in increasingly many research fields, the need for rapid on-site testing methods is also increasing. The test procedure often consists of three steps: Sample preparation, amplification, and detection.
Jingwen Wang, Han Jiang, Leiming Pan, Xiuying Gu, Chaogeng Xiao, Pengpeng Liu, Yulong Tang, Jiehong Fang, Xiaoqian Li, Chenze Lu   +9 more
doaj   +1 more source

Blood RNA Biomarker Signatures for Early Diagnosis and Prognosis in Ischemic and Hemorrhagic Stroke: The IBIS‐CT1 Study

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To evaluate the expression of nine blood RNA biomarkers in a clinical trial based on genes previously identified in an experimental monkey model of stroke for diagnosis feasibility and prognostication. Methods IBIS‐CT1 was a prospective longitudinal study enrolling patients with ischemic stroke (IS) or intracerebral hemorrhage (ICH ...
Salomé Retailleau, Marie Bruguet, Irina Viakhireva‐Dovganyuk, François‐Mathias Merrien, Denis Marechal, Aurore Jourdain, Philippe Goas, Jordan Coris, Julien Asselineau, Maëlys Consigny, Gérald Le Gac, Serge Timsit   +11 more
wiley   +1 more source

Isothermal Amplification of Rabies Virus Gene

Journal of Veterinary Medical Science, 2003
A sensitive and specific in situ amplification technique is needed to elucidate the dynamics of rabies virus in the body during the long incubation period after infection. To overcome the disadvantage of using the traditional reverse transcription (RT)-PCR in in situ studies, an isothermal nucleic acid sequence-based amplification (NASBA) technique was
SUGIYAMA, Makoto, ITO, Naoto, MINAMOTO, Nobuyuki   +2 more
openaire   +3 more sources