Results 31 to 40 of about 388,328 (197)
Rolling circle amplification as isothermal gene amplification in molecular diagnostics [PDF]
BioChip Journal, 2016 Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases.
Goo, Nam-In, Kim, Dong-Eun
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Practical Laboratory MedicineIsothermal nucleic acid amplification methods have gained prominence as a valuable innovation in molecular diagnostics. These methods function at a constant temperature without the requirement of thermal cycler apparatus, simplifying the amplification ...
Alazar Amare Amdiyee +1 more
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Advances in Nucleic Acid Amplification-Based Microfluidic Devices for Clinical Microbial Detection
Chemosensors, 2022 Accurate and timely detection of infectious pathogens is urgently needed for disease treatment and control of possible outbreaks worldwide. Conventional methods for pathogen detection are usually time-consuming and labor-intensive.
Thi Ngoc Diep Trinh, Nae Yoon Lee
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Isothermal amplification of long DNA segments by quadruplex priming amplification [PDF]
Analytical Methods, 2018 Amplification of long DNA segments with the highest possible specificity and lowest bias is one of the main goals of modern genomics.
Levan Lomidze +3 more
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Data in Brief, 2020 This article reports experimental data related to the research article entitled “Prevention of DNA multimerization using phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase” (R.R. Garafutdinov, A.R. Sakhabutdinova, M.
Ravil R. Garafutdinov +3 more
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Cross Priming Amplification: Mechanism and Optimization for Isothermal DNA Amplification [PDF]
Scientific Reports, 2012 CPA is a class of isothermal amplification reactions that is carried out by a strand displacement DNA polymerase and does not require an initial denaturation step or the addition of a nicking enzyme. At the assay temperature of 63°C, the formation of a primer-template hybrid at transient, spontaneous denaturation bubbles in the DNA template is favored ...
Gaolian Xu +8 more
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Scientific Reports, 2017 Isothermal amplification methods for detection of DNA and RNA targets have expanded significantly in recent years, promising a new wave of simple and rapid molecular diagnostics. Current isothermal methods result in the generation of short fragments (
Yinhua Zhang, Nathan A. Tanner
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A Sensitive Potentiometric Sensor for Isothermal Amplification-Coupled Detection of Nucleic Acids
Sensors, 2018 A disposable potentiometric sensor was newly developed for the amplification-coupled detection of nucleic acids. The hydrogen-ion is generally released during isothermal amplification of nucleic acids.
Kang-Ho Lee +4 more
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Assessing the presence of Wuchereria bancrofti in vector and human populations from urban communities in Conakry, Guinea [PDF]
, 2015 The Global Programme to Eliminate Lymphatic Filariasis was launched in 2000 with the goal of interrupting transmission of lymphatic filariasis (LF) through multiple rounds of mass drug administration (MDA).
Bockarie, Moses J. +25 more
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Molecular Methods for Identification and Quantification of Foodborne Pathogens
Molecules, 2022 Foodborne pathogens that enter the human food chain are a significant threat worldwide to human health. Timely and cost-effective detection of them became challenging for many countries that want to improve their detection and control of foodborne ...
Min Zhang +7 more
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