Results 21 to 30 of about 26,794 (255)

Rapid Detection of Virus Nucleic Acid via Isothermal Amplification on Plasmonic Enhanced Digitizing Biosensor

Biosensors, 2022
Rapid detection for infectious diseases is highly demanded in diagnosis and infection prevention. In this work, we introduced a plasmonic enhanced digitizing biosensor for the rapid detection of nucleic acids.
Shih-Chung Wei, Chia-Chen Chang, Tsung-Liang Chuang, Kung-Bin Sung, Chii-Wann Lin   +4 more
doaj   +1 more source

Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics.

PLoS ONE, 2020
A number of isothermal DNA amplification technologies claim to be ideal for point-of-need (PON) applications as they enable reactions to be performed using a single-temperature heat source (e.g. water bath).
Yiping Zou, Michael Glenn Mason, Jose Ramon Botella   +2 more
doaj   +1 more source

Comparative analysis of methods for isothermal amplification of nucleic acids

Журнал микробиологии, эпидемиологии и иммунобиологии, 2022
In this review, methods for isothermal amplification of nucleic acids are considered and analyzed, in particular, loop isothermal amplification of DNA and RNA (LAMP/RT-LAMP), helicase-dependent amplification (HDA) and recombinase polymerase amplification
O. S. Chemisova, O. A. Tsyrulina, A. L. Trukhachev, A. K. Noskov   +3 more
doaj   +1 more source

Real-time gene analysis based on a portable electrochemical microfluidic system

Electrochemistry Communications, 2020
Isothermal amplification, such as loop-mediated nucleic acid isothermal amplification (LAMP), is a highly promising technique that could revolutionize portable or point-of-care gene detection.
Yichen Liu, Baiyang Lu, Yidan Tang, Yan Du, Bingling Li   +4 more
doaj   +1 more source

Rolling Circle and Loop Mediated Isothermal Amplification Strategy for Ultrasensitive miRNA Detection

Separations, 2021
Rolling circle amplification (RCA) and loop mediated isothermal amplification (LAMP) were combined to establish the rolling circle and loop mediated isothermal amplification (RC-LAMP) method for miRNA detection.
Zheng Cao, Xianfeng Jiang, Guizhou Xiao, Mingcheng Xu, Hui Liu, Sheng Cai   +5 more
doaj   +1 more source

Advances in Nucleic Acid Amplification-Based Microfluidic Devices for Clinical Microbial Detection

Chemosensors, 2022
Accurate and timely detection of infectious pathogens is urgently needed for disease treatment and control of possible outbreaks worldwide. Conventional methods for pathogen detection are usually time-consuming and labor-intensive.
Thi Ngoc Diep Trinh, Nae Yoon Lee
doaj   +1 more source

Isothermal amplification of long DNA segments by quadruplex priming amplification [PDF]

Analytical Methods, 2018
Amplification of long DNA segments with the highest possible specificity and lowest bias is one of the main goals of modern genomics.
Levan Lomidze, Tyler H. Williford, Karin Musier-Forsyth, Besik Kankia   +3 more
openaire   +2 more sources

Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR

Practical Laboratory Medicine
Isothermal nucleic acid amplification methods have gained prominence as a valuable innovation in molecular diagnostics. These methods function at a constant temperature without the requirement of thermal cycler apparatus, simplifying the amplification ...
Alazar Amare Amdiyee, Tesfaye Sisay Tessema   +1 more
doaj   +1 more source

Cross Priming Amplification: Mechanism and Optimization for Isothermal DNA Amplification [PDF]

Scientific Reports, 2012
CPA is a class of isothermal amplification reactions that is carried out by a strand displacement DNA polymerase and does not require an initial denaturation step or the addition of a nicking enzyme. At the assay temperature of 63°C, the formation of a primer-template hybrid at transient, spontaneous denaturation bubbles in the DNA template is favored ...
Gaolian Xu, Lin Hu, Huayan Zhong, Hongying Wang, Sei-ichi Yusa, Tristen C. Weiss, Paul J. Romaniuk, Sam Pickerill, Qimin You   +8 more
openaire   +2 more sources

Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase

Data in Brief, 2020
This article reports experimental data related to the research article entitled “Prevention of DNA multimerization using phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase” (R.R. Garafutdinov, A.R. Sakhabutdinova, M.
Ravil R. Garafutdinov, Assol R. Sakhabutdinova, Maxim S. Kupryushkin, Dmitrii V. Pyshnyi   +3 more
doaj   +1 more source