Data in Brief, 2018 Loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are two rapid isothermal amplification methods for detecting three common fungal root pathogens of cool-season turfgrass: Gaeumannomyces avenae, Magnaporthiopsis
Brijesh B. Karakkat +5 more
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Isothermal amplification of long DNA segments by quadruplex priming amplification [PDF]
Analytical Methods, 2018 Amplification of long DNA segments with the highest possible specificity and lowest bias is one of the main goals of modern genomics.
Levan Lomidze +3 more
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Biosensors, 2022 Rapid detection for infectious diseases is highly demanded in diagnosis and infection prevention. In this work, we introduced a plasmonic enhanced digitizing biosensor for the rapid detection of nucleic acids.
Shih-Chung Wei +4 more
doaj +1 more source
Cross Priming Amplification: Mechanism and Optimization for Isothermal DNA Amplification [PDF]
Scientific Reports, 2012 CPA is a class of isothermal amplification reactions that is carried out by a strand displacement DNA polymerase and does not require an initial denaturation step or the addition of a nicking enzyme. At the assay temperature of 63°C, the formation of a primer-template hybrid at transient, spontaneous denaturation bubbles in the DNA template is favored ...
Gaolian Xu +8 more
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Lack of correlation between reaction speed and analytical sensitivity in isothermal amplification reveals the value of digital methods for optimization: validation using digital real-time RT-LAMP [PDF]
, 2015 In this paper, we asked if it is possible to identify the best primers and reaction conditions based on improvements in reaction speed when optimizing isothermal reactions.
Ismagilov, Rustem F. +3 more
core +2 more sources
Rolling circle amplification as isothermal gene amplification in molecular diagnostics [PDF]
BioChip Journal, 2016 Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases.
Goo, Nam-In, Kim, Dong-Eun
openaire +2 more sources
Point of Care Diagnosis of Multiple Schistosome Parasites: Species-specific DNA Detection in Urine by Loop-mediated Isothermal Amplification (LAMP) [PDF]
, 2017 Schistosomes are easily transmitted and high chance of repeat infection, so if control strategies based on targeted mass drug administration (MDA) are to succeed it is essential to have a test that is sensitive, accurate and simple to use.
Anyan, William K. +6 more
core +2 more sources
PLoS ONE, 2020 A number of isothermal DNA amplification technologies claim to be ideal for point-of-need (PON) applications as they enable reactions to be performed using a single-temperature heat source (e.g. water bath).
Yiping Zou +2 more
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Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense [PDF]
, 2008 Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique that rapidly amplifies target DNA under isothermal conditions. In the present study, a LAMP test was designed from the serum resistance-associated (SRA) gene of Trypanosoma brucei ...
Armstrong, T. +5 more
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Development of a recombinase polymerase amplification lateral flow assay for the detection of active Trypanosoma evansi infections [PDF]
, 2020 Author summary Neglected tropical diseases (NTDs) affecting humans and/or domestic animals severely impair the socio-economic development of endemic areas. One of these diseases, animal trypanosomosis, affects livestock and is caused by the parasites of
Goossens, Julie +5 more
core +1 more source