Results 21 to 30 of about 388,328 (197)

Using detergent to enhance detection sensitivity of African trypanosomes in human CSF and blood by Loop-Mediated Isothermal Amplification (LAMP) [PDF]

, 2011
<p><b>Background:</b> The loop-mediated isothermal amplification (LAMP) assay, with its advantages of simplicity, rapidity and cost effectiveness, has evolved as one of the most sensitive and specific methods for the detection of a ...
Gibson, W., Thekisoe, Oriel M M, Dumler, JS, Olga V. Nikolskaia, Olga V Nikolskaia, Liam J Morrison, Inoue, N., Noboru Inoue, Liam J. Morrison, Dennis J Grab, Oriel M. M. Thekisoe, Dumler J. Stephen, Grab, DJ, J. Stephen Dumler, Dumler, J.S., J Stephen Dumler, Morrison, L.J., Nikolskaia Olga V., Dumler, J Stephen, Nikolskaia, O.V., Nikolskaia, OV, Grab, D.J., Grab, Dennis J, Wendy Gibson, Morrison Liam J., Grab Dennis J., Inoue, N, Gibson, WC, Thekisoe, Oriel M. M., Oriel M M Thekisoe, Inoue, Noboru, Thekisoe Oriel M. M., Morrison, Liam J; id_orcid, Gibson Wendy, Thekisoe, OMM, Morrison, L, Inoue Noboru, Dennis J. Grab, Nikolskaia, Olga V, Gibson, Wendy   +39 more
core   +1 more source

Rapid Detection of Virus Nucleic Acid via Isothermal Amplification on Plasmonic Enhanced Digitizing Biosensor

Biosensors, 2022
Rapid detection for infectious diseases is highly demanded in diagnosis and infection prevention. In this work, we introduced a plasmonic enhanced digitizing biosensor for the rapid detection of nucleic acids.
Shih-Chung Wei, Chia-Chen Chang, Tsung-Liang Chuang, Kung-Bin Sung, Chii-Wann Lin   +4 more
doaj   +1 more source

Loop-mediated isothermal amplification (LAMP) for point-of-care detection of asymptomatic low-density malaria parasite carriers in Zanzibar. [PDF]

, 2015
BACKGROUND: Asymptomatic, low parasite density malaria infections are difficult to detect with currently available point-of-care diagnostics. This study piloted a loop-mediated isothermal amplification (LAMP) kit for field-friendly, high-throughput ...
Msellem, Mwinyi, Abass, Ali K, Mårtensson, Andreas, Schmidt, Berit, Abass, Ali K., Berit Aydin-Schmidt, Mwinyi Msellem, Nassor, Majda H, Cook, Jackie, Aydin-Schmidt, Berit, Nassor, Majda H., Björkman, Anders, Abdullah Ali, Elin Edlund, David Bell, Majda H Nassor, Andreas Mårtensson, Jackie Cook, Ali, Abdullah, Mårtensson, Andreas,, Anders Björkman, Gonzalez, Iveth J., González, Iveth J, Bell, David, Edlund, Elin, Ali K Abass, Iveth J González   +26 more
core   +1 more source

Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics.

PLoS ONE, 2020
A number of isothermal DNA amplification technologies claim to be ideal for point-of-need (PON) applications as they enable reactions to be performed using a single-temperature heat source (e.g. water bath).
Yiping Zou, Michael Glenn Mason, Jose Ramon Botella   +2 more
doaj   +1 more source

Applying loop-mediated isothermal amplification (LAMP) in the diagnosis of malaria, leishmaniasis and trypanosomiasis as point-of-care tests (POCTs) [PDF]

, 2018
One of the main objectives of the WHO is controlling transmission of parasitic protozoa vector- borne diseases. A quick and precise diagnosis is critical in selecting the optimal therapeutic regime that avoids unnecessary treatments and the emergence of ...
Dea-Ayuela, M. Auxiliadora, Lalatsa, Aikaterini, Galiana-Roselló, Cristina, Serrano, Dolores R.   +3 more
core   +2 more sources

Helicase‐dependent isothermal DNA amplification [PDF]

The EMBO Reports, 2004
Polymerase chain reaction is the most widely used method for in vitro DNA amplification. However, it requires thermocycling to separate two DNA strands. In vivo, DNA is replicated by DNA polymerases with various accessory proteins, including a DNA helicase that acts to separate duplex DNA.
Myriam, Vincent, Yan, Xu, Huimin, Kong
openaire   +2 more sources

Field-Capable Genetic Amplification Module

, 2020
Field-Capable Loop-Mediated Isothermal Amplification ...
Alex Gorsuch, Gorsuch, Margaret, Lee, Jonathan, Harikumar, Sanjiv, Kannard-Smith, Mackie   +4 more
core   +1 more source

Real-time gene analysis based on a portable electrochemical microfluidic system

Electrochemistry Communications, 2020
Isothermal amplification, such as loop-mediated nucleic acid isothermal amplification (LAMP), is a highly promising technique that could revolutionize portable or point-of-care gene detection.
Yichen Liu, Baiyang Lu, Yidan Tang, Yan Du, Bingling Li   +4 more
doaj   +1 more source

Comparative analysis of methods for isothermal amplification of nucleic acids

Журнал микробиологии, эпидемиологии и иммунобиологии, 2022
In this review, methods for isothermal amplification of nucleic acids are considered and analyzed, in particular, loop isothermal amplification of DNA and RNA (LAMP/RT-LAMP), helicase-dependent amplification (HDA) and recombinase polymerase amplification
O. S. Chemisova, O. A. Tsyrulina, A. L. Trukhachev, A. K. Noskov   +3 more
doaj   +1 more source

Rolling Circle and Loop Mediated Isothermal Amplification Strategy for Ultrasensitive miRNA Detection

Separations, 2021
Rolling circle amplification (RCA) and loop mediated isothermal amplification (LAMP) were combined to establish the rolling circle and loop mediated isothermal amplification (RC-LAMP) method for miRNA detection.
Zheng Cao, Xianfeng Jiang, Guizhou Xiao, Mingcheng Xu, Hui Liu, Sheng Cai   +5 more
doaj   +1 more source