Results 21 to 30 of about 33,911 (295)
Molecular Mechanisms Underlying CRISPR/Cas-Based Assays for Nucleic Acid Detection
Current Issues in Molecular Biology, 2023 Applied to investigate specific sequences, nucleic acid detection assays can help identify novel bacterial and viral infections. Most up-to-date systems combine isothermal amplification with Cas-mediated detection.
Denis N. Antropov, Grigory A. Stepanov
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Isothermal reactions for the amplification of oligonucleotides [PDF]
Proceedings of the National Academy of Sciences, 2003 We have devised a class of isothermal reactions for amplifying DNA. These homogeneous reactions rapidly synthesize short oligonucleotides (8–16 bases) specified by the sequence of an amplification template. Versions of the reactions can proceed in either a linear or an exponential amplification mode. Both of these reactions require
Jeffrey, Van Ness +2 more
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Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests
Biosensors, 2023 Isothermal nucleic acid amplification tests have recently gained popularity over polymerase chain reaction (PCR), as they only require a constant temperature and significantly simplify nucleic acid amplification.
Jocelyn Reynolds +4 more
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Journal of Infection in Developing Countries, 2021 Approximately 1.41 million people die annually due to tuberculosis. One of the main problems in Tuberculosis eradication is the development of resistance to various antibiotics.
Muhammad Habiburrahman +2 more
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Loop-mediated isothermal amplification of DNA [PDF]
Nucleic Acids Research, 2000 We have developed a novel method, termed loop-mediated isothermal amplification (LAMP), that amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA.
T, Notomi +6 more
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New Method of Isothermal, Hairpin Assisted, Primer Independent Amplification of DNA
BioChem, 2023 The isothermal amplification of nucleic acids refers to processes that quickly increase the amount of DNA at a constant temperature. These methods are mainly developed as alternatives to PCR for cases in which the application of a thermal cycler is not ...
Denis Sergeevich Naberezhnov +3 more
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Helicase‐dependent isothermal DNA amplification [PDF]
EMBO reports, 2004 Polymerase chain reaction is the most widely used method for in vitro DNA amplification. However, it requires thermocycling to separate two DNA strands. In vivo , DNA is replicated by DNA polymerases with various accessory proteins, including a DNA ...
Myriam, Vincent, Yan, Xu, Huimin, Kong
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BioTechniques, 2022 Coronavirus disease 2019 is a public health challenge requiring rapid testing for the detection of infections and transmission. Nucleic acid amplification tests targeting SARS coronavirus 2 (CoV2) are used to detect CoV2 in clinical samples.
Les Jones +3 more
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Isothermal amplifications – a comprehensive review on current methods [PDF]
Critical Reviews in Biochemistry and Molecular Biology, 2021 The introduction of nucleic acid amplification techniques has revolutionized the field of medical diagnostics in the last decade. The advent of PCR catalyzed the increasing application of DNA, not just for molecular cloning but also for molecular based diagnostics.
Jörn Glökler +3 more
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Rolling circle amplification as isothermal gene amplification in molecular diagnostics [PDF]
BioChip Journal, 2016 Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases.
Goo, Nam-In, Kim, Dong-Eun
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