Results 21 to 30 of about 2,464,363 (288)
Evaluating the Efficiency of REPLI-g?? Single Cell Kit for Trace DNA Amplification [PDF]
Fayixue Zazhi, 2019 Objective To evaluate the efficiency of REPLI-g?? Single Cell Kit for sample DNA amplification, and explore its application value in forensic trace DNA amplification. Methods Three DNA extraction kits were selected to extract DNA from peripheral blood of
XU Qian-nan, SHEN Qiong, ZHANG Jia-yi，et al.
doaj +1 more source
The Indian Journal of Agricultural Sciences, 2022 PCR amplification and detection of GC rich sequences in DNA is a challenge due to formation of secondary structures which resist denaturation, thereby stalling Taq DNA polymerases as well as affecting primer annealing.
GAURAV DHAWAN +6 more
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Nanomaterials, 2023 Quantitative real-time polymerase chain reaction (qPCR) is an important and extensively utilized technique in medical and biotechnological applications.
Khushbu Chauhan +3 more
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Review of Electrochemical DNA Biosensors for Detecting Food Borne Pathogens
Sensors, 2019 The vital importance of rapid and accurate detection of food borne pathogens has driven the development of biosensor to prevent food borne illness outbreaks.
Qiaoyun Wu +4 more
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DNA Amplification Techniques in Pharmacogenomics
Pharmacogenomics, 2011 The variable predisposition of patients, both to disease susceptibility and drug response, is well established. It is largely attributed to genetic, as well as epigenetic variations between individuals, which may be inherited or acquired. The most common variation in the human genome is the SNP, which occurs throughout the genome, both within coding ...
Morlighem, J.-E. +3 more
openaire +3 more sources
Scientific Reports, 2018 Single cell genome analysis methods are powerful tools to define features of single cells and to identify differences between them. Since the DNA amount of a single cell is very limited, cellular DNA usually needs to be amplified by whole-genome ...
Christiane Bäumer +4 more
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Kary Mullis - bright name in the history of science
Annals of Mechnikov's Institute, 2020 The article is devoted to the Karry Mullis– American biochemist, winner of Nobel Prize in Chemistry and to the history of discovery of polymerase chain reaction thanks to which rapid release of large number of specific DNA fragments becomes possible ...
I Korovaeva, N Popova
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Monodisperse Picoliter Droplets for Low-Bias and Contamination-Free Reactions in Single-Cell Whole Genome Amplification. [PDF]
PLoS ONE, 2015 Whole genome amplification (WGA) is essential for obtaining genome sequences from single bacterial cells because the quantity of template DNA contained in a single cell is very low.
Yohei Nishikawa +5 more
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Bio-Protocol, 2022 Spontaneous DNA damage frequently occurs on the human genome, and it could alter gene expression by inducing mutagenesis or epigenetic changes. Therefore, it is highly desired to profile DNA damage distribution on the human genome and identify the genes ...
Yichi Niu, Qiangyuan Zhu, Chenghang Zong
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Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source