Results 11 to 20 of about 2,464,363 (288)

Isothermal Amplification and Multimerization of DNA by Bst DNA Polymerase

BioTechniques, 2001
We have demonstrated the isothermal in vitro amplification and multimerization of several different linear DNA targets using only two primers and the strongly strand-displacing exonuclease-negative Bst DNA polymerase. This reaction has been termed linear
G.J. Hafner, I.C. Yang, L.C. Wolter, M.R. Stafford, P.M. Giffard   +4 more
doaj   +2 more sources

The use of Multiple Displacement Amplification to increase the detection and genotyping of Trypanosoma samples immobilised on FTA filters. [PDF]

, 2007
Whole genome amplification methods are a recently developed tool for amplifying DNA from limited template. We report its application in trypanosome infections, characterized by low parasitemias.
Sweeney, L., Turner, C.M.R., Morrison, L.J., Likeufack, A.C.L., Truc, P., Tait, A., McCormack, G., MacLeod, A.   +7 more
core   +8 more sources

Fabrication and modeling of a continuous-flow microfluidic device for on-chip DNA amplification [PDF]

, 2011
This paper was presented at the 3rd Micro and Nano Flows Conference (MNF2011), which was held at the Makedonia Palace Hotel, Thessaloniki in Greece.
Chatzandroulis, S, Mavraki, E, Tserepi, A, Moschou, DC, Kokkoris, G, 3rd Micro and Nano Flows Conference (MNF2011)   +5 more
core   +6 more sources

Forensic Applications of Whole Genome Amplification [PDF]

, 2011
Whole genome amplification (WGA) has been touted as a possible technique to augment fragment analysis of STRs in amplifying low-levels of DNA recovered from crime scenes or items.
Williams, Graham
core   +3 more sources

Optimization of Various ITS rDNA Amplification Protocol of Yeast Isolated from Giant Honey Beehives (Apis dorsata)

Jurnal Riset Biologi dan Aplikasinya, 2021
Indonesia is a country with high variability of microorganisms, including bacteria, yeast, and fungi. Yeast isolates could be isolated from the honeycomb of Apis dorsata. Molecular approaches were used to identify yeast using ribosomal DNA gene sequences,
Chumaidatul Choiriyah, S.Si, Nirmala Fitria Firdhausi, Esti Tyastirin, Yuanita Rachmawati, Moch. Irfan Hadi   +4 more
doaj   +1 more source

Effectiveness of blocking primers and a peptide nucleic acid (PNA) clamp for 18S metabarcoding dietary analysis of herbivorous fish.

PLoS ONE, 2022
The structure of food webs and carbon flow in aquatic ecosystems can be better understood by studying contributing factors such as the diets of herbivorous fish.
Chiho Homma, Daiki Inokuchi, Yohei Nakamura, Wilfredo H Uy, Kouhei Ohnishi, Haruo Yamaguchi, Masao Adachi   +6 more
doaj   +3 more sources

A mechanism of gene amplification driven by small DNA fragments.

PLoS Genetics, 2012
DNA amplification is a molecular process that increases the copy number of a chromosomal tract and often causes elevated expression of the amplified gene(s).
Kuntal Mukherjee, Francesca Storici
doaj   +1 more source

Mitochondrial dynamics-related genes DRP1 and OPA1 contributes to early diagnosis of cognitive impairment in diabetes

BMC Geriatrics, 2023
Background and aim DRP1 and OPA1 play important roles in mitochondrial fusion and fission. However, the role of DRP1 and OPA1 amplification in mitochondrial cognitive impairment has not been reported.
Mengqian Liu, Chen Gong, Xiaozhu Shen, Yi Jiang, Yiwen Xu, Wen Zhong, Yujiao Chen, Nan Dong, Jingxian Liao, Ning Yin   +9 more
doaj   +1 more source

TempliPhi, ⌽29 DNA Polymerase Based Rolling Circle Amplification of Templates for DNA Sequencing

BioTechniques, 2002
We have developed a novel, isothermal DNA amplification strategy that employs ⌽29 DNA polymerase and rolling circle amplification to generate high-quality templates for DNA sequencing reactions. The TempliPhi™ DNA amplification kits take advantage of the
John R. Nelson, Yuyang Christine Cai, Theresa L. Giesler, Joseph W. Farchaus, Shanmuuga T. Sundaram, Maria Ortiz-Rivera, Lou P. Hosta, Peter L. Hewitt, J. Anthony Mamone, C. Palaniappan, Carl W. Fuller   +10 more
doaj   +1 more source

New Method of Isothermal, Hairpin Assisted, Primer Independent Amplification of DNA

BioChem, 2023
The isothermal amplification of nucleic acids refers to processes that quickly increase the amount of DNA at a constant temperature. These methods are mainly developed as alternatives to PCR for cases in which the application of a thermal cycler is not ...
Denis Sergeevich Naberezhnov, Alexander Andreevich Alferov, Yuriy Borisovich Kuzmin, Nikolay Evgenievich Kushlinskii   +3 more
doaj   +1 more source