Results 41 to 50 of about 2,464,363 (288)

Accurate quantification of microorganisms in PCR-inhibiting environmental DNA extracts by a novel Internal Amplification Control approach using Biotrove OpenArrays [PDF]

, 2009
PCR-based detection assays are prone to inhibition by substances present in environmental samples, thereby potentially leading to inaccurate target quantification or false-negative results.
Gent-Pelzer, M. van, Gent-Pelzer, M.P.E., van, Van Doorn, R., Doorn, R. van, Speksnijder, A., Speksnijder, A.G.C.L., Klerks, M.M., Schoen, C.D., van Gent-Pelzer, M., Klerks, M., Gent-Pelzer van, M., Doorn, R., van, Kowalchuk, G.A., van Gent-Pelzer, M.P.E.   +13 more
core   +2 more sources

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Sweet enhancers of polymerase chain reaction.

PLoS ONE
Although faster and powerful, polymerase chain reaction (PCR) often failed to amplify targets efficiently. Numerous PCR enhancers have been used to increase the amplification efficiency of difficult DNA targets. However, there is no systematic comparison
Binghua Xie, Jia Chen, Zhounan Wang, Qiao Yin, Zhong-Min Dai   +4 more
doaj   +1 more source

Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for detection of Leishmania DNA in buffy coat from visceral leishmaniasis patients [PDF]

, 2012
BACKGROUND: Visceral leishmaniasis (VL) remains as one of the most neglected tropical diseases with over 60% of the world's total VL cases occurring in the Indian subcontinent.
Mondal, D., Khondaker Rifat Hasan Bhaskar, Tania Akther, Gerd Pluschke, Bhaskar, Khondaker Rifat Hasan, Pluschke Gerd, Akther, Tania, Salam, M. A., Mondal, Dinesh, Khan, M. G., Khan Md Gulam Musawwir, Salam Md Abdus, Akther Tania, Dinesh Mondal, Pluschke, G., Md Abdus Salam, Khan, Md. Gulam Musawwir, Salam, Md. Abdus, Mondal Dinesh, Bhaskar Khondaker Rifat Hasan, Md Gulam Musawwir Khan, Akther, T., Pluschke, Gerd, Bhaskar, K. R.   +23 more
core   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Single cell on-chip whole genome amplification via micropillar arrays for reduced amplification bias. [PDF]

PLoS ONE, 2018
Single cell whole genome amplification is susceptible to amplification biases that impact the accuracy of single cell sequencing data. To address this, we have developed a microfluidic device for the isolation and purification of genomic DNA from ...
Harvey C Tian, Jaime J Benitez, Harold G Craighead   +2 more
doaj   +1 more source

DNA extraction from formalin-fixed tissue: new light from the Deep-Sea [PDF]

, 2010
DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR
Thatje, S., Johnston, J., Johnston, David, Mackenzie-Dodds, Jackie, Clark, P., Hall, S., Palero, F., Mackenzie-Dodds, J., Hall, Sally, Clark, Paul, Palero, Ferran, Mackenzie Dodds, Jackie, Clark, Paul F., Palero, Ferran ; https://orcid.org/, Thatje, Sven   +14 more
core   +1 more source

The microbiome in human skin aging

FEBS Letters, EarlyView.
Age‐related skin changes encompass the well‐known visible phenotypic alterations, together with microbiome dysbiosis and a series of molecular aging hallmarks. These hallmarks characterize not only a fully stablished aged phenotype but also the skin aging process itself.
Manuel Huerta Arana, Cornelia Wiegand, Omid Omrani, Mario Fabri   +3 more
wiley   +1 more source

Locked nucleic acid inhibits amplification of contaminating DNA in real-time PCR

BioTechniques, 2005
Locked nucleic acid (LNA®) is a modified DNA with increased binding affinity for complementary DNA sequences. Our strategy was to use this property of LNA to inhibit undesired PCR amplification (e.g., from contaminating genomic DNA) in a cDNA-based assay.
Lone Hummelshoj, Lars P. Ryder, Hans O. Madsen, Lars K. Poulsen   +3 more
doaj   +1 more source

DMSO Improves the Ski-Slope Effect in Direct PCR

Applied Sciences, 2021
Analytical techniques such as DNA profiling are widely used in various fields, including forensic science, and novel technologies such as direct polymerase chain reaction (PCR) amplification are continuously being developed in order to acquire DNA ...
Joo-Young Kim, Ju Yeon Jung, Da-Hye Kim, Seohyun Moon, Won-Hae Lee, Byung-Won Chun, Dong-Ho Choi   +6 more
doaj   +1 more source