Results 121 to 130 of about 3,927,360 (403)

Modifications in FLAP's second cytosolic loop influence 5‐LOX interaction, inhibitor binding, and leukotriene formation

FEBS Letters, EarlyView.
The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp, Katharina Rataj, Stefanie König, Marcia E. Newcomer, Oliver Werz, Ulrike Garscha   +5 more
wiley   +1 more source

Affinity Purification of RNA: Sequence-Specific Capture by Nonionic Morpholino Probes

BioTechniques, 1997
Nucleic acid isolation for amplification-based diagnostics requires techniques that do not co-purify inhibitors of DNA polymerases. Also, other requirements for an ideal sample preparation technology include ease of use, capability for automation, high ...
J.M. Wages, G.M. Wages, P. Matthews, D. Weller, J. Summerton   +4 more
doaj   +1 more source

CIN2+ detection of the HPV DNA Array genotyping assay in comparison with the Cobas 4800 HPV test and cytology [PDF]

, 2019
BACKGROUND: HPV DNA Array is an E1-targeting PCR genotyping test, with capability of distinguishing 18 high-risk (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) and 11 low-risk HPV types (6, 11, 40, 42, 44, 54, 67, 69, 70, 85 ...
Agorastos, Theodoros, Chatzistamatiou, Kimon, Hempel, Matthias, Kaufmann, Andreas M., Krings, Amrei, Pesic, Aleksandra, Preyer, Rosemarie   +6 more
core   +1 more source

3'-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures.

Nucleic Acids Research, 2000
DNA probes with conjugated minor groove binder (MGB) groups form extremely stable duplexes with single-stranded DNA targets, allowing shorter probes to be used for hybridization based assays.
I. Kutyavin, I. Afonina, A. Mills, V. Gorn, E. Lukhtanov, E. Belousov, M. J. Singer, D. Walburger, S. Lokhov, A. Gall, R. Dempcy, M. Reed, R. B. Meyer, J. Hedgpeth   +13 more
semanticscholar   +1 more source

Brucella NyxA and NyxB dimerization enhances effector function during infection

FEBS Letters, EarlyView.
Brucella abortus thrives inside cells thanks to the translocation of effector proteins that fine‐tune cellular functions. NyxA and NyxB are two effectors that destabilize the nucleolar localization of their host target, SENP3. We show that the Nyx proteins directly interact with each other and that their dimerization is essential for their function ...
Lison Cancade‐Veyre, Arthur Louche, Francine C. A. Gérard, Laurent Terradot, Suzana P. Salcedo   +4 more
wiley   +1 more source

Generation of digoxigenin-incorporated probes to enhance DNA detection sensitivity

BioTechniques, 2016
Telomere length in humans has been correlated with cancer and age-related diseases. The standard method to measure telomere length relies on Southern blot analysis with radioactively or non-radioactively labeled probes containing several telomeric DNA ...
Tsung-Po Lai, Woodring E. Wright, Jerry W. Shay   +2 more
doaj   +1 more source

Experimental and theoretical studies of sequence effects on the fluctuation and melting of short DNA molecules [PDF]

, 2008
Understanding the melting of short DNA sequences probes DNA at the scale of the genetic code and raises questions which are very different from those posed by very long sequences, which have been extensively studied.
Cuesta-López S Angelov D Peyrard M, Cuesta-López S Wildes A Theodorakopoulos N Peyrard M, D Angelov, Douki T, M Peyrard, Montrichok A, Peyrard M, Peyrard M, S Cuesta-López, Saenger W, Weber G   +10 more
core   +4 more sources

The carboxylate “gripper” of the substrate is critical for C‐4 stereo‐inversion by UDP‐glucuronic acid 4‐epimerase

FEBS Letters, EarlyView.
UDP‐glucuronic acid 4‐epimerase (UGAepi) catalyzes NAD+‐dependent interconversion of UDP‐glucuronic acid (UDP‐GlcA) and UDP‐galacturonic acid (UDP‐GalA) via C4‐oxidation, 4‐keto‐intermediate rotation, and C4‐reduction. Here, Borg et al. examined the role of the substrate's carboxylate group in the enzymic mechanism by analyzing NADH‐dependent reduction
Annika J. E. Borg, Laura De Cnop, Bernd Nidetzky   +2 more
wiley   +1 more source

Detection of eae, bfpA, espA Genes on Diarrhoeagenic Strains of Escherichia coli Isolates

Indonesian Journal of Biotechnology, 2006
The Enteropathogenic Escherichia coli (EPEC) is one of pathogenic strain of diarrheagenic E. coli group in children and infant that occurs in developing countries. The significant virulence factors in pathogenic EPEC are eaeA (E. coli attachingeffacing ),
Agnes Sri Harti, Susi Iravati, Widya Asmara   +2 more
doaj   +1 more source

Charge transport in l-DNA probed by conducting-AFM, and relationship with its structure [PDF]

arXiv, 2003
We studied the electrical conductivity of DNA samples as function of the number of DNA molecules. We showed that the insulating gap (no current at low voltage) increases from ~1-2 V for bundles and large ropes to ~4-7 V for few DNA molecules. From the distance dependent variation of the current, a unique hopping distance of ~3 nm is calculated (polaron-
arxiv