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Diagnostic Applications of DNA Probes [PDF]
Infection Control & Hospital Epidemiology, 1991 The use of recombinant DNA technology to develop specific molecular (usually DNA) probes has provided medical and veterinary diagnostic laboratories with powerful new tools to enhance the diagnosis of infectious diseases, genetic disorders, and malignancies, as well as more sensitive and specific means to accomplish such tasks as tissue typing and ...
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Design aspects of bright red emissive silver nanoclusters/DNA probes for microRNA detection.
ACS Nano, 2012The influence of the nucleic acid secondary structure on the fast (1 h) formation of bright red emissive silver nanoclusters (AgNCs) in a DNA sequence (DNA-12nt-RED-160), designed for the detection of a microRNA sequence (RNA-miR160), was investigated ...
Pratik Shah +8 more
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Chemical Communications, 2012
Based on graphene oxide-protected DNA probes, we have developed a cyclic enzymatic amplification method for sensitive miRNA detection in complex biological samples.
Liang Cui +6 more
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Based on graphene oxide-protected DNA probes, we have developed a cyclic enzymatic amplification method for sensitive miRNA detection in complex biological samples.
Liang Cui +6 more
semanticscholar +1 more source
1992
Publisher Summary This chapter discusses the structure of DNA by enzymes and chemicals probing. Enzyme and chemical probing experiments have generated important insight into a number of altered DNA structures, including cruciform structures, left-handed Z-DNA, A tract-containing curved DNA, H-triplexes, G-tetrad structures, and parallel-stranded DNA,
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Publisher Summary This chapter discusses the structure of DNA by enzymes and chemicals probing. Enzyme and chemical probing experiments have generated important insight into a number of altered DNA structures, including cruciform structures, left-handed Z-DNA, A tract-containing curved DNA, H-triplexes, G-tetrad structures, and parallel-stranded DNA,
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Probing RNA Translocases with DNA
2014For some helicases, it is possible to investigate RNA translocase activity on DNA substrates because the enzyme acts on both substrates. Potassium permanganate (KMnO4) footprinting is a method used to chemically probe the conformation of DNA as well as the binding of proteins.
Kimberly A. Reynolds +2 more
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Analytical Chemistry, 2012
We present here a binding-induced fluorescence turn-on assay for protein detection. Key features of this assay include affinity binding-induced DNA hybridization and fluorescence enhancement of silver nanoclusters (Ag NCs) using guanine-rich DNA ...
Jingjing Li +4 more
semanticscholar +1 more source
We present here a binding-induced fluorescence turn-on assay for protein detection. Key features of this assay include affinity binding-induced DNA hybridization and fluorescence enhancement of silver nanoclusters (Ag NCs) using guanine-rich DNA ...
Jingjing Li +4 more
semanticscholar +1 more source
2003
Many research workers and clinicians working in routine laboratories are now using the "tools" developed by molecular biologists over the past 10 years to produce DNA sequences that can be used as probes to detect specific genes. Thus it is possible to screen food for salmonella (1) or other organisms in a test much more quickly than conventional ...
J. Lesley Woodhead +3 more
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Many research workers and clinicians working in routine laboratories are now using the "tools" developed by molecular biologists over the past 10 years to produce DNA sequences that can be used as probes to detect specific genes. Thus it is possible to screen food for salmonella (1) or other organisms in a test much more quickly than conventional ...
J. Lesley Woodhead +3 more
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Probing Amyloid-DNA Interaction with Nanofluidics
2022Nanofluidics is an emerging methodology to investigate single biomacromolecules without functionalization and/or attachment of the molecules to a substrate. In conjunction with fluorescence microscopy, it can be used to investigate structural and dynamical aspects of amyloid-DNA interaction.
Basak, Rajib +4 more
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“DNA Traffic Lights”: Concept of Wavelength‐Shifting DNA Probes and Application in an Aptasensor
ChemBioChem, 2012Add it and see it: The concept of "DNA traffic lights" for wavelength-shifting DNA probes has a great potential in the application of biosensors, for example, in DNA aptamers.
Carolin Holzhauser, H. Wagenknecht
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Small DNA circles as probes of DNA topology
Biochemical Society Transactions, 2013Small DNA circles can occur in Nature, for example as protein-constrained loops, and can be synthesized by a number of methods. Such small circles provide tractable systems for the study of the structure, thermodynamics and molecular dynamics of closed-circular DNA.
Anthony Maxwell +4 more
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