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DNA Probe Assay Based on Exonuclease III Digestion of Probes Hybridized on Target DNA
Analytical Biochemistry, 1995A quantitative DNA probe assay process has been developed that uses exonuclease III. The fluorophore-labeled DNA probe is hybridized with specific sequences of the target DNA and then enzymatically digested. As these probe hybridization and digestion cycle reactions are repeated at a fixed temperature, digested probes (shortened probes) accumulate in ...
Hideki Kambara, Kazunori Okano
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DNA Probes for the Anopheles punctulatus Complex
The American Journal of Tropical Medicine and Hygiene, 1996Genomic DNA probes were made for two recently identified members of the Anopheles punctulatus complex; Anopheles sp. near punctulatus from Papua New Guinea and Anopheles farauti No. 7 from the Solomon Islands. The probes are species-specific and with the use of 32P labeling sensitive enough so that a squash blot of only a small segment of the mosquito ...
Beebe, NW +4 more
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DNA Probes for Viral Diagnosis
1992Great excitement has surrounded the development of molecular hybridization techniques for viruses because of the potential of nucleic acid probes as diagnostic reagents. The need for rapid and accurate viral diagnosis has become particularly pressing with the recent availability of numerous drugs which have been clinically proven as effective in ...
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JAMA, 1987
The emergence of recombinant DNA technology in the 1970s has given rise to new diagnostic techniques based on DNA probes. The Food and Drug Administration's interest in these probes stems from its regulatory responsibilities regarding DNA probes as medical devices. The agency also sees DNA probes as valuable tools in meeting its mission of identifying,
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The emergence of recombinant DNA technology in the 1970s has given rise to new diagnostic techniques based on DNA probes. The Food and Drug Administration's interest in these probes stems from its regulatory responsibilities regarding DNA probes as medical devices. The agency also sees DNA probes as valuable tools in meeting its mission of identifying,
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Nature, 1977 DNA probe amplification methods
Journal of Virological Methods, 1991Isa K. Mushahwar, Larry G. Birkenmeyer
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