Results 71 to 80 of about 3,796,516 (329)

Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization

Nature Communications, 2019
Optimisation of nucleic acid probes and blocker strands can be laborious. Here the authors construct a theoretical model of competitive DNA hybridisation to design DNA probes for optimisation-free mutation detection.
Xin Chen, Na Liu, Liquan Liu, Wei Chen, Na Chen, Meng Lin, Jiaju Xu, Xing Zhou, Hongbo Wang, Meiping Zhao, Xianjin Xiao   +10 more
doaj   +1 more source

Parallel profiling of DNA methylation and hydroxymethylation highlights neuropathology-associated epigenetic variation in Alzheimer’s disease

Clinical Epigenetics, 2019
Background Alzheimer’s disease is a progressive neurodegenerative disorder that is hypothesized to involve epigenetic dysfunction. Previous studies of DNA modifications in Alzheimer’s disease have been unable to distinguish between DNA methylation and ...
Adam R. Smith, Rebecca G. Smith, Ehsan Pishva, Eilis Hannon, Janou A. Y. Roubroeks, Joe Burrage, Claire Troakes, Safa Al-Sarraj, Carolyn Sloan, Jonathan Mill, Daniel L. van den Hove, Katie Lunnon   +11 more
doaj   +1 more source

Real-Time PCR Detection of Bacillus anthracis by Lambda_Ba03 Prophage Genes

Проблемы особо опасных инфекций, 2022
The aim of the study was to develop a set of primers and fluorescent probes for the detection of two chromosomal targets of Bacillus anthracis using real-time PCR based on the lambda_Ba03 prophage genes.Materials and methods.
A. S. Nizkorodova, E. R. Mal’tseva, Zh. A. Berdygulova, D. A. Naizabaeva, S. A. Kuatbekova, A. V. Zhigailov, N. Abdolla, A. S. Mashzhan, I. A. Akhmetollaev, Yu. A. Skiba, S. M. Mamadaliev   +10 more
doaj   +1 more source

Sex chromosome positions in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probes [PDF]

, 1984
Two cloned repetitive DNA probes, pXBR and CY1, which bind preferentially to specific regions of the human X and Y chromosome, respectively, were used to study the distribution of the sex chromosomes in human lymphocyte nuclei by in situ hybridization ...
C. R. M�ller, CR M�ller, DE Comings, F Pera, F Vogel, G. A. Rappold, GA Rappold, GA Rappold, H-D Hager, H. D. Hager, JR Gosden, K. E. Davies, L Avivi, L Hens, L Hens, LM Kunkel, M Schmid, M Spaeter, MD Bennett, ML Law, P Goodfellow, PL Pearson, PS Gerhard, R Gagn�, R Lewin, T Cremer, T Cremer, T Yang, T. Cremer, T. Yang, V Kanda   +30 more
core   +1 more source

Probe walking: development of novel probes for DNA fingerprinting [PDF]

Human Genetics, 1989
The tandem repeat of a 28-base-pair (bp) sequence downstream of the human c-Ha-ras-1 oncogene was studied as a probe for DNA fingerprinting. Multiple hypervariable patterns were observed by Southern hybridization at low stringency. The patterns were specific to individuals, indicating the availability of the 28-bp repeat as a probe for DNA ...
Shintaroh Ueda, Keiko Washio, Shogo Misawa   +2 more
openaire   +3 more sources

FoxO1 signaling in B cell malignancies and its therapeutic targeting

FEBS Letters, EarlyView.
FoxO1 has context‐specific tumor suppressor or oncogenic character in myeloid and B cell malignancies. This includes tumor‐promoting properties such as stemness maintenance and DNA damage tolerance in acute leukemias, or regulation of cell proliferation and survival, or migration in mature B cell malignancies.
Krystof Hlavac, Petra Pavelkova, Laura Ondrisova, Marek Mraz   +3 more
wiley   +1 more source

Changes in the subgingival biofilm composition after coronally positioned flap

Journal of Applied Oral Science, 2011
OBJECTIVES: This study evaluated the effects of coronally positioned flap (CPF) on the subgingival biofilm composition. MATERIAL AND METHODS: Twenty-two subjects with gingival recessions were treated with CPF. Clinical parameters were assessed before and
Jadson Almeida Lima, Vanessa Renata Santos, Magda Feres, Luciene Cristina de Figueiredo, Poliana Mendes Duarte   +4 more
doaj   +1 more source

The Bnapus50K array: a quick and versatile genotyping tool for Brassica napus genomic breeding and research

G3: Genes, Genomes, Genetics, 2021
Rapeseed is a globally cultivated commercial crop, primarily grown for its oil. High-density single nucleotide polymorphism (SNP) arrays are widely used as a standard genotyping tool for rapeseed research, including for gene mapping, genome-wide ...
Qing Xiao, Huadong Wang, Nuan Song, Zewen Yu, Khan Imran, Weibo Xie, Shuqing Qiu, Fasong Zhou, Jing Wen, Cheng Dai, Chaozhi Ma, Jinxing Tu, Jinxiong Shen, Tingdong Fu, Bin Yi   +14 more
doaj   +1 more source

Specific and non specific hybridization of oligonucleotide probes on microarrays [PDF]

, 2005
Gene expression analysis by means of microarrays is based on the sequence specific binding of mRNA to DNA oligonucleotide probes and its measurement using fluorescent labels. The binding of RNA fragments involving other sequences than the intended target
Binder, Hans, Preibisch, Stephan
core   +3 more sources

Elucidation of interface interactions between a dehydratase domain and an acyl carrier protein in cremimycin polyketide synthase

FEBS Letters, EarlyView.
In modular polyketide synthases, the dehydratase (DH) domain catalyzes the dehydration reaction of the β‐hydroxyacyl unit attached to the cognate acyl carrier protein (ACP) domain. However, it is unclear how DH interacts with ACP during the reaction. In this study, we identified DH–ACP interface residues, providing the first detailed insights into DH ...
Kaede Kotagiri, Haruka Tachibana, Daisuke Kawasaki, Taichi Chisuga, Toma Kashima, Shinya Fushinobu, Fumitaka Kudo, Tadashi Eguchi, Akimasa Miyanaga   +8 more
wiley   +1 more source