Results 71 to 80 of about 3,917,543 (254)

Dinuclear Ruthenium(II) Complexes as Two-Photon, Time-Resolved Emission Microscopy Probes for Cellular DNA

open access: yesAngewandte Chemie, 2014
The first transition-metal complex-based two-photon absorbing luminescence lifetime probes for cellular DNA are presented. This allows cell imaging of DNA free from endogenous fluorophores and potentially facilitates deep tissue imaging.
Elizabeth Baggaley   +9 more
semanticscholar   +1 more source

Labeling of Unique Sequences in Double-Stranded DNA at Sites of Vicinal Nicks Generated by Nicking Endonucleases [PDF]

open access: yes, 2008
We describe a new approach for labeling of unique sequences within dsDNA under nondenaturing conditions. The method is based on the site-specific formation of vicinal nicks, which are created by nicking endonucleases (NEases) at specified DNA sites on ...
Frank-Kamenetskii, Maxim D., Kuhn, Heiko
core   +8 more sources

Real-Time PCR Detection of Bacillus anthracis by Lambda_Ba03 Prophage Genes

open access: yesПроблемы особо опасных инфекций, 2022
The aim of the study was to develop a set of primers and fluorescent probes for the detection of two chromosomal targets of Bacillus anthracis using real-time PCR based on the lambda_Ba03 prophage genes.Materials and methods.
A. S. Nizkorodova   +10 more
doaj   +1 more source

Conductivity of DNA probed by conducting-atomic force microscopy: effects of contact electrode, DNA structure, surface interactions [PDF]

open access: yes, 2004
We studied the electrical conductivity of DNA molecules with conducting atomic force microscopy as a function of the chemical nature of the substrate surfaces, the nature of the electrical contact, and the number of DNA molecules (from a few molecules, to ropes and large fibers containing up to ~ 106 molecules).
arxiv   +1 more source

Chromosome-specific DNA Repeat Probes [PDF]

open access: yesJournal of Histochemistry & Cytochemistry, 2006
In research as well as in clinical applications, fluorescence in situ hybridization (FISH) has gained increasing popularity as a highly sensitive technique to study cytogenetic changes. Today, hundreds of commercially available DNA probes serve the basic needs of the biomedical research community. Widespread applications, however, are often limited by
Adolf Baumgartner   +3 more
openaire   +5 more sources

Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes.

open access: yesNucleic Acids Research, 1991
Dispersed repetitive DNA sequences have been described recently in eubacteria. To assess the distribution and evolutionary conservation of two distinct prokaryotic repetitive elements, consensus oligonucleotides were used in polymerase chain reaction ...
J. Versalovic, T. Koeuth, J. Lupski
semanticscholar   +1 more source

Changes in the subgingival biofilm composition after coronally positioned flap

open access: yesJournal of Applied Oral Science, 2011
OBJECTIVES: This study evaluated the effects of coronally positioned flap (CPF) on the subgingival biofilm composition. MATERIAL AND METHODS: Twenty-two subjects with gingival recessions were treated with CPF. Clinical parameters were assessed before and
Jadson Almeida Lima   +4 more
doaj   +1 more source

The Bnapus50K array: a quick and versatile genotyping tool for Brassica napus genomic breeding and research

open access: yesG3: Genes, Genomes, Genetics, 2021
Rapeseed is a globally cultivated commercial crop, primarily grown for its oil. High-density single nucleotide polymorphism (SNP) arrays are widely used as a standard genotyping tool for rapeseed research, including for gene mapping, genome-wide ...
Qing Xiao   +14 more
doaj   +1 more source

Probe walking: development of novel probes for DNA fingerprinting [PDF]

open access: yesHuman Genetics, 1989
The tandem repeat of a 28-base-pair (bp) sequence downstream of the human c-Ha-ras-1 oncogene was studied as a probe for DNA fingerprinting. Multiple hypervariable patterns were observed by Southern hybridization at low stringency. The patterns were specific to individuals, indicating the availability of the 28-bp repeat as a probe for DNA ...
Shintaroh Ueda   +2 more
openaire   +3 more sources

DNA hybridization to mismatched templates: a chip study [PDF]

open access: yes, 2001
High-density oligonucleotide arrays are among the most rapidly expanding technologies in biology today. In the {\sl GeneChip} system, the reconstruction of the target concentration depends upon the differential signal generated from hybridizing the ...
A. Bonincontro   +19 more
core   +2 more sources

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