Results 61 to 70 of about 776,748 (351)

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Changes in the subgingival biofilm composition after coronally positioned flap

Journal of Applied Oral Science, 2011
OBJECTIVES: This study evaluated the effects of coronally positioned flap (CPF) on the subgingival biofilm composition. MATERIAL AND METHODS: Twenty-two subjects with gingival recessions were treated with CPF. Clinical parameters were assessed before and
Jadson Almeida Lima, Vanessa Renata Santos, Magda Feres, Luciene Cristina de Figueiredo, Poliana Mendes Duarte   +4 more
doaj   +1 more source

Probing Rad51-DNA interactions by changing DNA twist [PDF]

Nucleic Acids Research, 2012
In eukaryotes, Rad51 protein is responsible for the recombinational repair of double-strand DNA breaks. Rad51 monomers cooperatively assemble on exonuclease-processed broken ends forming helical nucleo-protein filaments that can pair with homologous regions of sister chromatids.
Atwell, Scott, Disseau, Ludovic, Stasiak, Alicja Z, Stasiak, Andrzej, Renodon-Cornière, Axelle, Takahashi, Masayuki, Viovy, Jean-Louis, Cappello, Giovanni   +7 more
openaire   +7 more sources

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Imaging Unique DNA Sequences in Individual Cells Using a CRISPR-Cas9-Based, Split Luciferase Biosensor

Frontiers in Genome Editing, 2022
An extensive arsenal of biosensing tools has been developed based on the clustered regularly interspaced short palindromic repeat (CRISPR) platform, including those that detect specific DNA sequences both in vitro and in live cells.
Nicholas G. Heath, Nicholas G. Heath, Nicholas G. Heath, Henriette O’Geen, Henriette O’Geen, Nicole B. Halmai, Nicole B. Halmai, Jacob E. Corn, Jacob E. Corn, David J. Segal, David J. Segal, David J. Segal   +11 more
doaj   +1 more source

A Field Study Using the Polymerase Chain Reaction (Pcr) to Screen for Brugia Microfilariae in Human and Animal Blood [PDF]

, 1989
Blood samples from 43 humans and 14 cats positive with Brugia microfilariae were analyzed in a field study in Tanjung Pinang, Indonesia. The study used the polymerase chain reaction (PCR) to compare the sensitivity of radioactive and biotinylated species-
Glover, J. (Janet), Landry, D. (David), McReynolds, L. A. (Larry), Partono, F. (Felix), Supali, T. (Taniawati), Szabo, S. (Susanne), Williams, S. A. (Steven)   +6 more
core  

Recovering Sparse Signals Using Sparse Measurement Matrices in Compressed DNA Microarrays [PDF]

, 2008
Microarrays (DNA, protein, etc.) are massively parallel affinity-based biosensors capable of detecting and quantifying a large number of different genomic particles simultaneously.
Hassibi, Babak, Misra, Sidhant, Parvaresh, Farzad, Vikalo, Haris   +3 more
core   +2 more sources

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning

BMC Biotechnology, 2019
Background Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length limit of current DNA padlock probe
Syukri Shukor, Alfred Tamayo, Lorenzo Tosi, H. Benjamin Larman, Biju Parekkadan   +4 more
doaj   +1 more source

Real-Time PCR Detection of Bacillus anthracis by Lambda_Ba03 Prophage Genes

Проблемы особо опасных инфекций, 2022
The aim of the study was to develop a set of primers and fluorescent probes for the detection of two chromosomal targets of Bacillus anthracis using real-time PCR based on the lambda_Ba03 prophage genes.Materials and methods.
A. S. Nizkorodova, E. R. Mal’tseva, Zh. A. Berdygulova, D. A. Naizabaeva, S. A. Kuatbekova, A. V. Zhigailov, N. Abdolla, A. S. Mashzhan, I. A. Akhmetollaev, Yu. A. Skiba, S. M. Mamadaliev   +10 more
doaj   +1 more source