Results 61 to 70 of about 2,143,309 (303)

DNA probes design and foodborne pathogen detection methods

open access: yes, 2020
On April 30, 2020, Early Stage Researchers (ESRs) from INESC-MN and BioSense Institute conducted the 2nd OJC. The meeting focused on discussing articles related to the IPANEMA SOL2 topic: Paper-based detection of Campylobacter spp.
Minja Mladenović   +4 more
core   +1 more source

Precision Optical Measurements of DNA Structure and Synthesis [PDF]

open access: yes, 2007
The structure and synthesis of DNA is important. Traditionally,high-resolution structures are solved with X-ray crystallography, and kinetic measurements are taken with consumption of large amount of reagents.
Ziyang Ma, Ma, Ziyang
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

open access: yesFEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura   +2 more
wiley   +1 more source

Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries [PDF]

open access: yes, 1988
A method of in situ hybridization for visualizing individual human chromosomes from pter to qter, both in metaphase spreads and interphase nuclei, is reported.
Cremer, Thomas   +4 more
core   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

open access: yesFEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz   +11 more
wiley   +1 more source

Characterization of Giardia isolates using a non-radiolabeled DNA probe, and correlation with the results of isoenzyme analysis [PDF]

open access: yes, 1989
Forty-seven isolates, identified morphologically as Giardia duodenalis, were compared by restriction endonuclease analysis of DNA with hybridization to a non-radiolabeled probe. Seven schizodemes were distinguished, compared to 15 zymodemes identified by
Alan J. Lymbery   +5 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

open access: yesFEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa   +3 more
wiley   +1 more source

The Bnapus50K array: a quick and versatile genotyping tool for Brassica napus genomic breeding and research

open access: yesG3: Genes, Genomes, Genetics, 2021
Rapeseed is a globally cultivated commercial crop, primarily grown for its oil. High-density single nucleotide polymorphism (SNP) arrays are widely used as a standard genotyping tool for rapeseed research, including for gene mapping, genome-wide ...
Qing Xiao   +14 more
doaj   +1 more source

Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens

open access: yesBMC Infectious Diseases, 2003
Background We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.
Miller Donna L   +7 more
doaj   +1 more source

Direct probing: covalent attachment of probe DNA to double-stranded target DNA [PDF]

open access: yesNucleic Acids Research, 1998
We report a novel procedure, which can be applied to probing of specific DNA, for covalently attaching probe DNA to complementary sequences in double-stranded target DNA. Employing hairpin-like oligonucleotide probes in combination with successive use of recA protein and DNA ligase, probes can be attached directly to target DNA molecules without ...
J, Fujiwara, M, Oishi
openaire   +2 more sources

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