Generation of digoxigenin-incorporated probes to enhance DNA detection sensitivity
BioTechniques, 2016 Telomere length in humans has been correlated with cancer and age-related diseases. The standard method to measure telomere length relies on Southern blot analysis with radioactively or non-radioactively labeled probes containing several telomeric DNA ...
Tsung-Po Lai +2 more
doaj +1 more source
Multicolor fluorescence in situ hybridization on metaphase chromosomes and interphase Halo-preparations using cosmid and YAC clones for the simultaneous high resolution mapping of deletions in the dystrophin gene [PDF]
, 1994 We report on multicolor fluorescence in situ hybridization protocols for the simultaneous visualization of deletion-prone regions for carrier detection of Duchenne/ Becker (DMD/BMD) muscular dystrophy.
Blonden, Lau +6 more
core +1 more source
Detection of eae, bfpA, espA Genes on Diarrhoeagenic Strains of Escherichia coli Isolates
Indonesian Journal of Biotechnology, 2006 The Enteropathogenic Escherichia coli (EPEC) is one of pathogenic strain of diarrheagenic E. coli group in children and infant that occurs in developing countries. The significant virulence factors in pathogenic EPEC are eaeA (E. coli attachingeffacing ),
Agnes Sri Harti +2 more
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Rapid mapping of chromosomal breakpoints: from blood to BAC in 20 days [PDF]
, 2009 Structural chromosome aberrations and associated segmental or chromosomal aneusomies are major causes of reproductive failure in humans. Despite the fact that carriers of reciprocal balanced translocation often have no other clinical symptoms or ...
Baumgartner, Adolf +7 more
core +2 more sources
Automated High-Throughput Probe Production for DNA Microarray Analysis
BioTechniques, 2003 DNA microarrays have become an established tool for gene expression profiling. Construction of these microarrays using immobilized cDNAs is a common experimental strategy.
P.R. Hoyt +5 more
doaj +1 more source
A Field Study Using the Polymerase Chain Reaction (Pcr) to Screen for Brugia Microfilariae in Human and Animal Blood [PDF]
, 1989 Blood samples from 43 humans and 14 cats positive with Brugia microfilariae were analyzed in a field study in Tanjung Pinang, Indonesia. The study used the polymerase chain reaction (PCR) to compare the sensitivity of radioactive and biotinylated species-
Glover, J. (Janet) +6 more
core
A Low Density Microarray Method for the Identification of Human Papillomavirus Type 18 Variants
Sensors, 2013 We describe a novel microarray based-method for the screening of oncogenic human papillomavirus 18 (HPV-18) molecular variants. Due to the fact that sequencing methodology may underestimate samples containing more than one variant we designed a specific ...
Aracely López-Monteon +6 more
doaj +1 more source
A repetitive probe for FISH analysis of bovine interphase nuclei
Genetics Selection Evolution, 2000 The purpose of this study was to generate repetitive DNA sequence probes for the analysis of interphase nuclei by fluorescent in situ hybridisation (FISH).
Cribiu Edmond +5 more
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Unsupervised Classification for Tiling Arrays: ChIP-chip and Transcriptome
, 2011 Tiling arrays make possible a large scale exploration of the genome thanks to probes which cover the whole genome with very high density until 2 000 000 probes.
Aubourg, Sébastien +4 more
core +3 more sources
BioTechniques, 2001 In quantitative real-time PCR assays, fluorophor-labeled oligonucleotide probes are employed to generate sequence-specific signals for the quantitative evaluation. Whereas TaqMan® probes have to be hydrolyzed during PCR by the endonucleolytic activity of
Jochen Wilhelm +2 more
doaj +1 more source