Results 81 to 90 of about 3,796,516 (329)
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
Proceedings of the National Academy of Sciences of the United States of America, 1980 A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed. Poly(A)+ and ribosomal RNAs transfer efficiently to nitrocellulose paper in high salt (3 M NaCl/0.3 M trisodium citrate ...
P. S. Thomas
semanticscholar +1 more source
Characteristics of the Kelch domain containing (KLHDC) subfamily and relationships with diseases
FEBS Letters, EarlyView.The Kelch protein superfamily includes 63 members, with the KLHDC subfamily having 10 proteins. While their functions are not fully understood, recent advances in KLHDC2's structure and role in protein degradation have highlighted its potential for drug development, especially in PROTAC therapies.
Courtney Pilcher +6 more
wiley +1 more source
The Scientific World Journal, 2003 Analyses of complex microbial communities are becoming increasingly important. Bottlenecks in these analyses, however, are the tools to actually describe the biodiversity.
Knut Rudi +3 more
doaj +1 more source
Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning
BMC Biotechnology, 2019 Background Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length limit of current DNA padlock probe
Syukri Shukor +4 more
doaj +1 more source
FEBS Letters, EarlyView.LHCPs are transported to the thylakoid membrane via the (cp)SRP pathway. This process involves a transit complex of (cp)SRP43, (cp)SRP54 and LHCP, which interacts with (cp)FtsY and Alb3 at the membrane. GTP hydrolysis by (cp)SRP54 and (cp)FtsY triggers complex dissociation.
Victor Zegarra +7 more
wiley +1 more source
DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus [PDF]
, 1995 We used three short repetitive nucleotide sequences [(GTG)5, (TAC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted Citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic ...
Asif, M +7 more
core +3 more sources
Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes
Scientific Reports, 2018 We have redesigned probes for in situ proximity ligation assay (PLA), resulting in more efficient localized detection of target proteins. In situ PLA depends on recognition of target proteins by pairs of antibody-oligonucleotide conjugates (PLA probes ...
Axel Klaesson +10 more
doaj +1 more source
Genome-wide analysis of DNA methylation in buccal cells: a study of monozygotic twins and mQTLs
Epigenetics & Chromatin, 2018 Background DNA methylation arrays are widely used in epigenome-wide association studies and methylation quantitative trait locus (mQTL) studies. Here, we performed the first genome-wide analysis of monozygotic (MZ) twin correlations and mQTLs on data ...
Jenny van Dongen +13 more
doaj +1 more source
DNA probe for identification of Streptococcus pneumoniae [PDF]
Journal of Clinical Microbiology, 1989 A total of 287 clinical isolates of Streptococcus pneumoniae (pneumococcus) were tested for their ability to undergo autolysis when treated with sodium deoxycholate. The test was positive for all but one isolate, strain DOC-1. This autolysis required the activity of an enzyme which is unique and characteristic of S.
Pozzi G, Oggioni MR, Tomasz A.
openaire +4 more sources
Genome Biology, 2016 In recent years the Illumina HumanMethylation450 (HM450) BeadChip has provided a user-friendly platform to profile DNA methylation in human samples. However, HM450 lacked coverage of distal regulatory elements.
R. Pidsley +9 more
semanticscholar +1 more source