Results 71 to 80 of about 3,431,424 (346)
Chemistry Proceedings, 2021 The work seeks to identify molecules with inhibitory activity against the DNA gyrase of Gram-negative microorganisms resistant to fluoroquinolones. Previously designed compounds were used to study antimicrobial potential in silico.
Manuel Alejandro Coba-Males +2 more
doaj +1 more source
Regulated Eukaryotic DNA Replication Origin Firing with Purified Proteins
Nature, 2015 Eukaryotic cells initiate DNA replication from multiple origins, which must be tightly regulated to promote precise genome duplication in every cell cycle.
Joseph T. P. Yeeles +4 more
semanticscholar +1 more source
Organization of DNA Replication [PDF]
Cold Spring Harbor Perspectives in Biology, 2010 The discovery of the DNA double helix structure half a century ago immediately suggested a mechanism for its duplication by semi-conservative copying of the nucleotide sequence into two DNA daughter strands. Shortly after, a second fundamental step toward the elucidation of the mechanism of DNA replication was taken with the isolation of the first ...
M. Cristina Cardoso +2 more
openaire +3 more sources
FEBS Letters, EarlyView.Atovaquone is an antimalarial requiring potentiation for sufficient efficacy. We pursued strategies to enhance its activity, showing that 4‐nitrobenzoate inhibits 4‐hydroxybenzoate polyprenyltransferase, decreasing ubiquinone biosynthesis. Since atovaquone competes with ubiquinol in mitochondria, 4‐nitrobenzoate facilitates its action, potentiating ...
Ignasi Bofill Verdaguer +7 more
wiley +1 more source
FEBS Letters, EarlyView.Cells must clear mislocalized or faulty proteins from membranes to survive. The AAA+ ATPase Msp1 performs this task, but dissecting how its six subunits work together is challenging. We engineered linked dimers with varied numbers of functional subunits to reveal how Msp1 subunits cooperate and use energy to extract proteins from the lipid bilayer ...
Deepika Gaur +5 more
wiley +1 more source
DNA unwinding mechanism of a eukaryotic replicative CMG helicase
Nature Communications, 2020 The DNA duplex is known to be split apart in a steric exclusion manner during replication, but the specific mechanism has remained unclear. Here the authors present a cryo-EM structure of a eukaryotic replicative CMG helicase on forked DNA, revealing the
Zuanning Yuan +5 more
doaj +1 more source
Centromeric DNA replication reconstitution reveals DNA loops and ATR checkpoint suppression
Nature Cell Biology, 2016 Half of the human genome is made up of repetitive DNA. However, mechanisms underlying replication of chromosome regions containing repetitive DNA are poorly understood.
Antoine Aze +4 more
semanticscholar +1 more source
Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source
The Replication of Polyoma DNA
Journal of General Virology, 1973 Summary Measurement of replicating molecules of polyoma virus DNA after digestion with endonuclease R1 shows that DNA replication is bidirectional, starting predominantly at a specific site. In both large plaque and small plaque polyoma virus DNA this site is 29 ± 2% of the total length of the DNA from the cleavage site of the endonuclease R1.
Annelie Wilde +2 more
openaire +3 more sources
Reciprocal control of viral infection and phosphoinositide dynamics
FEBS Letters, EarlyView.Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley +1 more source