Translocation-coupled DNA cleavage by the Type ISP restriction-modification enzymes [PDF]
Nature Chemical Biology, 2015 Production of endonucleolytic double-strand DNA breaks requires separate strand cleavage events. Although catalytic mechanisms for simple, dimeric endonucleases are known, there are many complex nuclease machines that are poorly understood. Here we studied the single polypeptide Type ISP restriction-modification (RM) enzymes, which cleave random DNA ...
Mahesh Kumar Chand +7 more
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DNA cleavage by Type ISP Restriction–Modification enzymes is initially targeted to the 3′-5′ strand [PDF]
Nucleic Acids Research, 2012 The mechanism by which a double-stranded DNA break is produced following collision of two translocating Type I Restriction-Modification enzymes is not fully understood. Here, we demonstrate that the related Type ISP Restriction-Modification enzymes LlaGI and LlaBIII can cooperate to cleave DNA following convergent translocation and collision.
Kara van Aelst +2 more
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DNA duplexes with reactive dialdehyde groups as novel reagents for cross-linking to restriction- modification enzymes [PDF]
Nucleic Acids Research, 1997 To create new, effective reagents for affinity modification of restriction-modification (R-M) enzymes, a regioselective method for reactive dialdehyde group incorporation into oligonucleotides, based on insertion of a 1-beta-D-galactopyranosylthymine residue, has been developed. We synthesized DNA duplex analogs of the substrates of the Eco RII and Mva
Maxim G. Brevnov
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DNA-mediated coupling of ATPase, translocase and nuclease activities of a Type ISP restriction-modification enzyme [PDF]
Nucleic Acids Research, 2020 Abstract Enzymes involved in nucleic acid transactions often have a helicase-like ATPase coordinating and driving their functional activities, but our understanding of the mechanistic details of their coordination is limited. For example, DNA cleavage by the antiphage defense system Type ISP restriction-modification enzyme requires ...
Mahesh Kumar Chand +3 more
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A prediction of the amino acids and structures involved in DNA recognition by type I DNA restriction and modification enzymes [PDF]
, 1997 Simon Sturrock
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A new affinity reagent for the site-specific, covalent attachment of DNA to active-site nucleophiles: application to theEcoRI andRsrl restriction and modification enzymes [PDF]
, 1992 Andrei A. Purmal +2 more
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Basis for changes in DNA recognition by the Type I DNA restriction and modification enzymes
, 1989 C P Price +4 more
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Rapid Analysis of DNA Methylation Using New Restriction Enzyme Sites Created by Bisulfite Modification [PDF]
, 1996 Roya Sadri, Peter J. Hornsby
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Differential detection of transposable elements between Saccharum species
Genetics and Molecular Biology, 2013 Cultivars of sugarcane (Saccharum) are hybrids between species S. officinarum (x = 10, 2n = 8x = 80) and S. spontaneum (x=8,2n=5-16x =40-128). These accessions have 100 to 130 chromosomes, 80-85% of which are derived from S.
Marislane Carvalho Paz de Souza +2 more
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