Results 31 to 40 of about 87,449 (274)

Broadness and specificity: ArdB, ArdA, and Ocr against various restriction-modification systems

Frontiers in Microbiology, 2023
ArdB, ArdA, and Ocr proteins inhibit the endonuclease activity of the type I restriction-modification enzymes (RMI). In this study, we evaluated the ability of ArdB, ArdA, and Ocr to inhibit different subtypes of Escherichia coli RMI systems (IA, IB, and
Anna A. Kudryavtseva, Eva Cséfalvay, Evgeniy Yu Gnuchikh, Darya D. Yanovskaya, Mikhail A. Skutel, Artem B. Isaev, Sergey V. Bazhenov, Sergey V. Bazhenov, Sergey V. Bazhenov, Anna A. Utkina, Ilya V. Manukhov, Ilya V. Manukhov, Ilya V. Manukhov   +12 more
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Correction: Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities

PLOS Biology, 2016
[This corrects the article DOI: 10.1371/journal.pbio.1002442.].
The PLOS Biology Staff
openalex   +4 more sources

The helical domain of the EcoR124I motor subunit participates in ATPase activity and dsDNA translocation [PDF]

PeerJ, 2017
Type I restriction-modification enzymes are multisubunit, multifunctional molecular machines that recognize specific DNA target sequences, and their multisubunit organization underlies their multifunctionality.
Vitali Bialevich, Dhiraj Sinha, Katsiaryna Shamayeva, Alena Guzanova, David Řeha, Eva Csefalvay, Jannette Carey, Marie Weiserova, Rüdiger H. Ettrich   +8 more
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The Hyperthermophilic Restriction-Modification Systems of Thermococcus kodakarensis Protect Genome Integrity

Frontiers in Microbiology, 2021
Thermococcus kodakarensis (T. kodakarensis), a hyperthermophilic, genetically accessible model archaeon, encodes two putative restriction modification (R-M) defense systems, TkoI and TkoII.
Kelly M. Zatopek, Brett W. Burkhart, Richard D. Morgan, Alexandra M. Gehring, Kristin A. Scott, Thomas J. Santangelo, Andrew F. Gardner   +6 more
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Interaction between EcoRII restriction/modification enzymes and synthetic DNA fragments. Synthesis of substrates containing a single recognition site [PDF]

Biopolymers and Cell, 1987
Светлана А. Кузнецова, Е. А. Кубарева, T. S. Oretskaya, Nina G. Dolinnaya, N. F. Krynetskaya, Е. С. Громова, З.А. Шабарова, Dieter Cech   +7 more
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Time-resolved fluorescence of 2-aminopurine in DNA duplexes in the presence of the EcoP15I Type III restriction–modification enzyme

Biochemical and Biophysical Research Communications, 2014
EcoP15I is a Type III DNA restriction and modification enzyme of Escherichia coli. We show that it contains two modification (Mod) subunits for sequence-specific methylation of DNA and one copy of a restriction endonuclease (Res) subunit for cleavage of DNA containing unmethylated target sequences. Previously the Mod2 dimer in the presence of cofactors
Long Ma, Xiaohua Wu, Geoffrey G. Wilson, Anita C. Jones, David T. F. Dryden   +4 more
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Fused eco29kIR- and M genes coding for a fully functional hybrid polypeptide as a model of molecular evolution of restriction-modification systems

BMC Evolutionary Biology, 2011
Background The discovery of restriction endonucleases and modification DNA methyltransferases, key instruments of genetic engineering, opened a new era of molecular biology through development of the recombinant DNA technology.
Solonin Alexander S, Mokrishcheva Marina L, Nikitin Dmitri V   +2 more
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Functional coupling of duplex translocation to DNA cleavage in a type I restriction enzyme. [PDF]

PLoS ONE, 2015
Type I restriction-modification enzymes are multifunctional heteromeric complexes with DNA cleavage and ATP-dependent DNA translocation activities located on motor subunit HsdR.
Eva Csefalvay, Mikalai Lapkouski, Alena Guzanova, Ladislav Csefalvay, Tatsiana Baikova, Igor Shevelev, Vitali Bialevich, Katsiaryna Shamayeva, Pavel Janscak, Ivana Kuta Smatanova, Santosh Panjikar, Jannette Carey, Marie Weiserova, Rüdiger Ettrich   +13 more
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An alternative approach to study the enzymatic specificities of the CfrBI restriction–modification system

Heliyon, 2019
Restriction–modification systems (RMS) are the main gene-engineering tools and a suitable model to study the molecular mechanisms of catalysis and DNA–protein interactions.
Marina V. Zakharova, Irina V. Beletskaya, Elena M. Ibryashkina, Alexander S. Solonin   +3 more
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Structural and functional analysis of the symmetrical Type I restriction endonuclease R.EcoR124I_NT

PLoS ONE, 2012
Type I restriction-modification (RM) systems are comprised of two multi-subunit enzymes, the methyltransferase (∼160 kDa), responsible for methylation of DNA, and the restriction endonuclease (∼400 kDa), responsible for DNA cleavage. Both enzymes share a
James E Taylor, Anna Swiderska, Jean-Baptiste Artero, Philip Callow, Geoff Kneale   +4 more
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