Structural basis of transcription inhibition by the DNA mimic protein Ocr of bacteriophage T7
eLife, 2020 Bacteriophage T7 infects Escherichia coli and evades the host restriction/modification system. The Ocr protein of T7 was shown to exist as a dimer mimicking DNA and to bind to host restriction enzymes, thus preventing the degradation of the viral genome ...
Fuzhou Ye +6 more
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mSystems, 2023 With the advance of high-throughput sequencing, the molecular basis of coevolutionary interaction between viruses and host microorganisms is predominantly elucidated by in silico genomic analyses, which revealed potential communication of genetic ...
Yi Zhang +6 more
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Internuclear gene silencing in Phytophthora infestans is established through chromatin remodelling [PDF]
, 2008 In the plant pathogen Phytophthora infestans, nuclear integration of inf1 transgenic DNA sequences results in internuclear gene silencing of inf1. Although silencing is regulated at the transcriptional level, it also affects transcription from other ...
Appiah, A.A. +7 more
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Mm19, a Mycoplasma meleagridis Major Surface Nuclease that Is Related to the RE_AlwI Superfamily of Endonucleases. [PDF]
PLoS ONE, 2016 Mycoplasma meleagridis infection is widespread in turkeys, causing poor growth and feathering, airsacculitis, osteodystrophy, and reduction in hatchability. Like most mycoplasma species, M.
Elhem Yacoub +1 more
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Altering under-represented DNA sequences elevates bacterial transformation efficiency
mBio, 2023 A cornerstone of bacterial molecular biology is the ability to genetically manipulate the microbe under study. Many bacteria are difficult to manipulate genetically, a phenotype due in part to robust removal of newly acquired DNA, for example, by ...
Shuai Hu +5 more
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Detection of DNA methylation in eucaryotic cells.
Folia Histochemica et Cytobiologica, 2008 The methods of molecular biology allow for analyzing the methylation pattern in the whole genome and in particular genes. We differentiate methylated sequences from unmethylated ones by means of cutting the genomic template with methylation-sensitive ...
Lech Chyczewski +7 more
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CRISPR/Cas9-mediated phage resistance is not impeded by the DNA modifications of phage T4. [PDF]
PLoS ONE, 2014 Bacteria rely on two known DNA-level defenses against their bacteriophage predators: restriction-modification and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated (Cas) systems.
Stephanie J Yaung +2 more
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Analysis of the subunit assembly of the type IC restriction-modification enzyme EcoR124I [PDF]
, 1998 Type I restriction-modification (R-M) enzymes are composed of three different subunits, of which HsdS determines DNA specificity, HsdM is responsible for DNA methylation and HsdR is required for restriction.
Dryden, D T F, Firman, K, Janscak, P
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Simple innovative adaptor to improve genome walking with convenient PCR
Journal of Genetic Engineering and Biotechnology, 2020 Background Various polymerase chain reaction (PCR)-based methods have been applied for the development of genome walking (GW) technique. These methods which could be based on the application of restriction enzymes or primers have various efficiencies to ...
Seyedeh-Samira Ashrafmansouri +3 more
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Heterologous Expression and High Degree Purification of the Restriction Endonuclease SauUSI
Bio-Protocol, 2022 Mechanisms that target and destroy foreign nucleic acids are major barriers to horizontal gene transfer (HGT) in prokaryotes. Amongst them, restriction-modification (R-M) systems are found in ≥75% of the sequenced genomes in Bacteria and Archaea.
Vinayak Tumuluri, Kayarat Saikrishnan
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