Results 51 to 60 of about 87,449 (274)
The DNA Methylome of the Hyperthermoacidophilic Crenarchaeon Sulfolobus acidocaldarius
Frontiers in Microbiology, 2018 DNA methylation is the most common epigenetic modification observed in the genomic DNA (gDNA) of prokaryotes and eukaryotes. Methylated nucleobases, N6-methyl-adenine (m6A), N4-methyl-cytosine (m4C), and 5-methyl-cytosine (m5C), detected on gDNA ...
Mohea Couturier, Ann-Christin Lindås
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EcoBLMcrX, a classical modification-dependent restriction enzyme in Escherichia coli B: Characterization in vivo and in vitro with a new approach to cleavage site determination. [PDF]
PLoS ONE, 2017 Here we characterize the modification-dependent restriction enzyme (MDE) EcoBLMcrX in vivo, in vitro and in its genomic environment. MDE cleavage of modified DNAs protects prokaryote populations from lethal infection by bacteriophage with highly modified
Alexey Fomenkov +5 more
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BMC Microbiology, 2008 Background Type I restriction-modification (R-M) systems are the most complex restriction enzymes discovered to date. Recent years have witnessed a renaissance of interest in R-M enzymes Type I.
Ryu Junichi, Weiserová Marie
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Scientific Reports, 2017 DNA phosphorothioate (PT) modification is a sulfur modification on the backbone of DNA introduced by the proteins DndA-E. It has been detected within many bacteria isolates and metagenomic datasets, including human pathogens, and is considered to be ...
Yan Yang +9 more
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Restriction modification systems in archaea: A panoramic outlook
HeliyonRestriction modification (RM) systems are one of the ubiquitous yet primitive defense responses employed by bacteria and archaea with the primary role of safeguarding themselves against invading bacteriophages.
Pallavi Gulati +4 more
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Restriction and modification of deoxyarchaeosine (dG+)-containing phage 9 g DNA
Scientific Reports, 2017 E. coli phage 9 g contains the modified base deoxyarchaeosine (dG+) in its genome. The phage encodes its own primase, DNA ligase, DNA polymerase, and enzymes necessary to synthesize and incorporate dG+.
Rebecca Tsai +3 more
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Interactions of Dnd Proteins involved in bacterial DNA phosphorothioate modification
Frontiers in Microbiology, 2015 DNA phosphorothioation (PT) is the first discovered physiological DNA backbone modification, in which a non-bridging oxygen atom of the phosphodiester bond is replaced with a sulfur atom in Rp (Rectus for plane) configuration. PT modification is governed
Wei eXiong +3 more
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Reciprocal control of viral infection and phosphoinositide dynamics
FEBS Letters, EarlyView.Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
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Adapterama III: Quadruple-indexed, double/triple-enzyme RADseq libraries (2RAD/3RAD) [PDF]
PeerJ, 2019 Molecular ecologists frequently use genome reduction strategies that rely upon restriction enzyme digestion of genomic DNA to sample consistent portions of the genome from many individuals (e.g., RADseq, GBS). However, researchers often find the existing
Natalia J. Bayona-Vásquez +12 more
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A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
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