Results 121 to 130 of about 1,359,741 (317)

An efficient method for DNA extraction from Cladosporioid fungi [PDF]

, 2010
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Bahkali, A.H., Abd-Elsalam, K.A., Wit, P.J.G.M., de, Moslem, M.A.   +3 more
core   +1 more source

AVITI sequencing of a four-generation CEPH/Utah pedigree confirms low mutation rates at homopolymer loci despite their low sequence complexity

Genome Biology
Background Short tandem repeats (STRs) and homopolymers are among the most mutable loci in the human genome. Despite their presumed mutability owing to replication slippage, homopolymer loci exhibit lower mutation rates and minimal paternal age effects ...
Hannah C. Happ, Thomas A. Sasani, Derek Warner, Deborah W. Neklason, Aaron R. Quinlan   +4 more
doaj   +1 more source

Network divergence analysis identifies adaptive gene modules and two orthogonal vulnerability axes in pancreatic cancer

Molecular Oncology, EarlyView.
Tumors contain diverse cellular states whose behavior is shaped by context‐dependent gene coordination. By comparing gene–gene relationships across biological contexts, we identify adaptive transcriptional modules that reorganize into distinct vulnerability axes.
Brian Nelson, Lyanne Delgado‐Coka, Natalia Marchenko, Luisa F. Escobar‐Hoyos, Kenneth R. Shroyer, Alisa Yurovsky, Trey Ideker, Gábor Balázsi, Thomas MacCarthy, Scott Powers   +9 more
wiley   +1 more source

Optimizing Splicing Junction Detection in Next Generation Sequencing Data on a Virtual-GRID Infrastructure [PDF]

, 2012
The new protocol for sequencing the messenger RNA in a cell, named RNA-seq produce millions of short sequence fragments. Next Generation Sequencing technology allows more accurate analysis but increase needs in term of computational resources. This paper
Provenzano, R., Provenzano R., Terzo, Olivier, Mossucca, L., Acquaviva, Andrea, Ficarra, Elisa, Mossucca L, Abate, Francesco   +7 more
core  

Second-generation environmental sequencing unmasks marine metazoan biodiversity [PDF]

, 2010
Biodiversity is of crucial importance for ecosystem functioning, sustainability and resilience, but the magnitude and organization of marine diversity at a range of spatial and taxonomic scales are undefined.
Lambshead, P. John D., Sung, Way, Harriet F. Johnson, Sung, W., Blaxter, M. L., Way Sung, Power, Deborah M., Thomas, W. K., W. Kelley Thomas, Lambshead, P John D, Margaret Packer, Blaxter, Mark L., Fonseca, Vera G., Packer, Margaret, Power, Deborah, Thomas, W Kelley, Carvalho, Gary, Mark L. Blaxter, Creer, Simon, Simon P. Neill, Johnson, Harriet F, Simon Creer, Fonseca, V. G., Thomas, W. Kelley, Blaxter, Mark L, Deborah M. Power, Neill, Simon P, Creer, S., Carvalho, Gary R, Johnson, Harriet F., P. John D. Lambshead, Lambshead, P. J. D., Vera G. Fonseca, Gary R. Carvalho, Carvalho, Gary R., Fonseca, Vera G, Neill, Simon P., Power, Deborah M   +37 more
core   +1 more source

The first case of Babesia gibsoni infection in a dog in Poland

Veterinární Medicína, 2018
Canine babesiosis is a tickborne, protozoal, haemoparasitic disease that can cause varying degrees of haemolytic anaemia, splenomegaly, thrombocytopenia and fever. Babesia organisms are frequently classified as either large or small.
L. Adaszek, P. Lyp, P. Poblocki, M. Skrzypczak, L. Mazurek, S. Winiarczyk   +5 more
doaj   +1 more source

COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells

Molecular Oncology, EarlyView.
This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos, Gilar Gorji‐bahri, Lejla Gradjan, Julia Zajac, Kacper Gil, Yvonne Thokozile Nduku, Anna M. Blom   +6 more
wiley   +1 more source

Counting absolute number of molecules using unique molecular identifiers [PDF]

, 2011
Advances in molecular biology have made it easy to identify different DNA or RNA species and to copy them. Identification of nucleic acid species can be accomplished by reading the DNA sequence; currently millions of molecules can be sequenced in a ...
Teemu Kivioja, Martin Bonke, Sten Linnarsson, Kasper Karlsson, Anna Vähärautio, Jussi Taipale   +5 more
core   +1 more source

ALLPATHS: de novo assembly of whole-genome shotgun microreads [PDF]

, 2008
New DNA sequencing technologies deliver data at dramatically lower costs but demand new analytical methods to take full advantage of the very short reads that they produce.
Kleber, M, Nusbaum, C, MacCallum, I, Jaffe, DB, Lander, ES, Shlyakhter, IA, Belmonte, MK, Butler, J   +7 more
core   +1 more source

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source