Results 31 to 40 of about 1,995,166 (228)

Structural basis for allosteric regulation of Human Topoisomerase IIα

Nature Communications, 2021
Type 2 DNA topoisomerases (Top2) regulates DNA topology during DNA replication, transcription, and chromosome segregation. Here the authors describe a complete structure of the catalytic core of the human Topo IIα bound to DNA and etoposide, providing ...
Arnaud Vanden Broeck, Christophe Lotz, Robert Drillien, Léa Haas, Claire Bedez, Valérie Lamour   +5 more
doaj   +1 more source

Archaea: A Gold Mine for Topoisomerase Diversity

Frontiers in Microbiology, 2021
The control of DNA topology is a prerequisite for all the DNA transactions such as DNA replication, repair, recombination, and transcription. This global control is carried out by essential enzymes, named DNA-topoisomerases, that are mandatory for the ...
Florence Garnier, Florence Garnier, Mohea Couturier, Hélène Débat, Hélène Débat, Marc Nadal, Marc Nadal   +6 more
doaj   +1 more source

Physical Characterization of Gemini Surfactant-Based Synthetic Vectors for the Delivery of Linear Covalently Closed (LCC) DNA Ministrings. [PDF]

PLoS ONE, 2015
In combination with novel linear covalently closed (LCC) DNA minivectors, referred to as DNA ministrings, a gemini surfactant-based synthetic vector for gene delivery has been shown to exhibit enhanced delivery and bioavailability while offering a ...
Chi Hong Sum, Nafiseh Nafissi, Roderick A Slavcev, Shawn Wettig   +3 more
doaj   +1 more source

TEX264 coordinates p97- and SPRTN-mediated resolution of topoisomerase 1-DNA adducts

Nature Communications, 2020
Eukaryotic topoisomerase 1 (TOP1) regulates DNA topology to ensure efficient DNA replication and transcription. Here, the authors reveal insights into the molecular resolution of topoisomerase 1-DNA adducts by TEX264, p97 and SPRTN.
John Fielden, Katherine Wiseman, Ignacio Torrecilla, Shudong Li, Samuel Hume, Shih-Chieh Chiang, Annamaria Ruggiano, Abhay Narayan Singh, Raimundo Freire, Sylvana Hassanieh, Enric Domingo, Iolanda Vendrell, Roman Fischer, Benedikt M. Kessler, Timothy S. Maughan, Sherif F. El-Khamisy, Kristijan Ramadan   +16 more
doaj   +1 more source

Role of the central cations in the mechanical unfolding of DNA and RNA G-quadruplexes. [PDF]

, 2015
Cations are known to mediate diverse interactions in nucleic acids duplexes but they are critical in the arrangement of four-stranded structures. Here, we use all-atom molecular dynamics simulations with explicit solvent to analyse the mechanical ...
Bergues-Pupo, AE, Fiasconaro A., Ana Elisa Bergues-pupo, Bergues-Pupo, A.E., Arias-Gonzalez, J. R., Morón, M.C., Fiasconaro, A., Bergues Pupo, Ana Elisa, Arias-Gonzalez, J. Ricardo, Arias-Gonzalez, J., Fiasconaro, Alessandro, Bergues-Pupo, A., Falo, Fernando, Morón, MC, Fiasconaro, A, Bergues-Pupo A.E., Morón, M. Carmen, Falo, F, J. Ricardo Arias-gonzalez, Ro Fiasconaro, Morón M.C., Falo, F., Arias-Gonzalez, JR, Falo F., Arias-Gonzalez J.R., O Falo   +25 more
core   +1 more source

Beyond Node Degree: Evaluating AS Topology Models [PDF]

, 2012
This is the accepted version of 'Beyond Node Degree: Evaluating AS Topology Models', archived originally at arXiv:0807.2023v1 [cs.NI] 13 July 2008.Many models have been proposed to generate Internet Autonomous System (AS) topologies, most of which make ...
Mortier, R, Fay, D, Haddadi, H, Uhlig, S, Rio, M, Jamakovic, A, Maennel, O, Moore, AW   +7 more
core   +2 more sources

Nucleosomal DNA has topological memory

Nature Communications
One elusive aspect of the chromosome architecture is how it constrains the DNA topology. Nucleosomes stabilise negative DNA supercoils by restraining a DNA linking number difference (∆Lk) of about −1.26.
Joana Segura, Ofelia Díaz-Ingelmo, Belén Martínez-García, Alba Ayats-Fraile, Christoforos Nikolaou, Joaquim Roca   +5 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Topology and geometry of DNA

, 2022
Bu tez 7 Bölümden oluşmaktadır. 1. Bölümde; Düğüm Teorisi ve temel düğüm hareketlerinin tanımları verildi. Bölüm 2'nin özü, DNA ve DNA'nın topolojik yapısı tarafından düzenlenen daha biyokimyasal bir bağlamdaki DNA ve düğümler hakkındadır. 3.
HAVVA NUR KARABAY
core  

Changes in the Topology of DNA Replication Intermediates: In vivo vs In vitro [PDF]

, 2021
Most of the methods used to analyze DNA, including electrophoresis, electron microscopy or atomic force microscopy, involve de-proteinization, and it is well known that the removal of proteins affects DNA topology.
Schvartzman, Jorge Bernardo, Fernández Nestosa, María José, Martínez, Víctor, Hernández, Pablo, Krimer, Dora B.   +4 more
core