Results 71 to 80 of about 1,893,090 (266)

Increasing the activity of DNAzyme based on the telomeric sequence: 2’-OMe-RNA and LNA modifications

Open Chemistry, 2019
2’-OMe-RNA analogues and LNA point modifications of DNA oligonucleotides were applied for the modulation of the G-quadruplex topology and enhancement of peroxidase activity of the resulting DNAzymes.
Kosman J., Żukowski K., Juskowiak B.
doaj   +1 more source

The unique DNA topology and DNA topoisomerases of hyperthermophilic archaea [PDF]

FEMS Microbiology Reviews, 1996
Hyperthermophilic archaea exhibit a unique pattern of DNA topoisomerase activities. They have a peculiar enzyme, reverse gyrase, which introduces positive superturns into DNA at the expense of ATP. This enzyme has been found in all hyperthermophiles tested so far (including Bacteria) but never in mesophiles.
P, Forterre, A, Bergerat, P, Lopez-Garcia   +2 more
openaire   +2 more sources

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

A benzothiazolium salt fluorophore for topology-specific discrimination of non-B DNA structures

Results in Chemistry
Accurate detection of non-B DNA structures remains challenging yet critical for understanding their roles in gene regulation, genome stability, and disease mechanisms.
Yawen Hou, Ruofeng Xu, Yujiao Hou, Yida Du, Keyi Gao, Jianlei Shen, Bin Chen   +6 more
doaj   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei [PDF]

, 1992
Tissue-specific patterns of methylated deoxycytidine residues in the mammalian genome are preserved by postreplicative methylation of newly synthesized DNA. DNA methyltransferase (MTase) is here shown to associate with replication foci during S phase but
Heinz-Ulrich Weier, Page, A.W., Weier, H.U., Andrea W. Page, Bestor, Timothy H., Bestor, T. H., Heinrich Leonhardt, Page, A. W., Page, Andrea W., Leonhardt, Heinrich, Weier, H. U., Weier, Heinz-Ulrich, Bestor, T.H., Timothy H. Bestor, Leonhardt, H.   +14 more
core   +2 more sources

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Preferential binding of hot spot mutant p53 proteins to supercoiled DNA in vitro and in cells.

PLoS ONE, 2013
Hot spot mutant p53 (mutp53) proteins exert oncogenic gain-of-function activities. Binding of mutp53 to DNA is assumed to be involved in mutp53-mediated repression or activation of several mutp53 target genes.
Marie Brázdová, Lucie Navrátilová, Vlastimil Tichý, Kateřina Němcová, Matej Lexa, Roman Hrstka, Petr Pečinka, Matej Adámik, Borivoj Vojtesek, Emil Paleček, Wolfgang Deppert, Miroslav Fojta   +11 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Role of DNA Topology in Biological Machines and Evolutionarily Related Biological Functions: Chemical Chirality in Site-Specific DNA Recombination

, 2019
Difference topology: The DNA path within a high-order DNA-protein assembly can be revealed from the topology of DNA knots and links formed by the action of Flp or Cre site-specific recombinase on target sites placed close to (and flanking) the assembly ...
Jayaram, Makkuni
core   +1 more source