Results 151 to 160 of about 1,575 (172)

Escherichia coli Expression, Purification, Crystallization, and Structure Determination of Bacterial Cohesin–Dockerin Complexes

2012
Cellulosomes are highly efficient nanomachines that play a fundamental role during the anaerobic deconstruction of complex plant cell wall carbohydrates. The assembly of these complex nanomachines results from the very tight binding of repetitive cohesin modules, located in a noncatalytic molecular scaffold, and dockerin domains located at the C ...
Bras JLA, Carvalho AL, Viegas A, Najmudin S, Alves VD, Prates JAM, Ferreira LMA, Romao MJ, Gilbert HJ, Fontes CMGA   +9 more
openaire   +3 more sources

Crystal structure of a cohesin module from Clostridium cellulolyticum: implications for dockerin recognition

Journal of Molecular Biology, 2000
In the assembly of the Clostridium cellulolyticum cellulosome, the multiple cohesin modules of the scaffolding protein CipC serve as receptors for cellulolytic enzymes which bear a dockerin module. The X-ray structure of a type I C. cellulolyticum cohesin module (Cc-cohesin) has been solved using molecular replacement, and refined at 2.0 A resolution ...
S, Spinelli, H P, Fiérobe, A, Belaïch, J P, Belaïch, B, Henrissat, C, Cambillau   +5 more
openaire   +2 more sources

Indirect ELISA‐based approach for comparative measurement of high‐affinity cohesin–dockerin interactions

Journal of Molecular Recognition, 2012
The interaction between the cohesin and dockerin modules serves to attach cellulolytic enzymes (carrying dockerins) to non‐catalytic scaffoldin units (carrying multiple cohesins) in cellulosome, a multienzyme plant cell‐wall degrading complex. This interaction is species‐specific, for example, the enzyme‐borne dockerin from Clostridium thermocellum ...
Michal, Slutzki, Yoav, Barak, Dan, Reshef, Ora, Schueler-Furman, Raphael, Lamed, Edward A, Bayer   +5 more
openaire   +2 more sources

Characterization of a Double Dockerin from the Cellulosome of the Anaerobic Fungus Piromyces equi

Journal of Molecular Biology, 2007
The assembly into supramolecular complexes of proteins having complementary activities is central to cellular function. One such complex of considerable biological and industrial significance is the plant cell wall-degrading apparatus of anaerobic microorganisms, termed the cellulosome.
Nagy T, Tunnicliffe RB, Higgins LD, Walters C, Gilbert HJ, Williamson MP   +5 more
openaire   +4 more sources

Characterization of a cellulosome dockerin domain from the anaerobic fungus Piromyces equi.

Nature structural biology, 2001
The recycling of photosynthetically fixed carbon in plant cell walls is a key microbial process. In anaerobes, the degradation is carried out by a high molecular weight multifunctional complex termed the cellulosome. This consists of a number of independent enzyme components, each of which contains a conserved dockerin domain, which functions to bind ...
Raghothama S, Eberhardt RY, Simpson P, Wigelsworth D, White P, Hazlewood GP, Nagy T, Gilbert HJ, Williamson MP   +8 more
openaire   +2 more sources

Structure and function relationships in novel cohesin-dockerin complexes

2015
ABSTRACT - Relações estrutura-função em novos complexos coesina-doquerina. - As interacções coesina-doquerina são responsáveis pela organização de um complexo multi-enzimático concebido para a degradação de glúcidos complexos da parede celular vegetal e secretado por bactérias anaeróbias, denominado por celulossoma.
openaire   +1 more source

Determination of binding affinity upon mutation for type I dockerin–cohesin complexes from Clostridium thermocellum and Clostridium cellulolyticum using deep sequencing

Proteins: Structure, Function, and Bioinformatics, 2016
Caitlin A. Kowalsky, Timothy A. Whitehead   +1 more
semanticscholar   +1 more source

Site-Specific and High-Loading Immobilization of Proteins by Using Cohesin-Dockerin and CBM-Cellulose Interactions.

Bioconjugate chemistry, 2016
Mei Li, Y. Yue, Zi-jian Zhang, Zaiyu Wang, T. Tan, Lihai Fan   +5 more
semanticscholar   +1 more source

Covalent and Orthogonal Cohesin–Dockerin Interactions Enabled by Intermolecular Disulfide Bonds for Hyperthermostable Cellulase Assembly

Industrial & Engineering Chemistry Research
Luke Bugada, Ponnandy Prabhu, Reid Bailey, Fei Wen   +3 more
semanticscholar   +1 more source

Functional characterization of a dockerin-containing expansin-like protein from the anaerobic fungus Neocallimastix californiae.

Biotechnology for Biofuels and Bioproducts
Taru Koitto, Anna Pohto, Elizaveta Sidorova, Thu V. Vuong, Merja Penttilä, Emma R. Master   +5 more
semanticscholar   +1 more source