Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in Embryonic Stem Cells
Cell Reports, 2020 Summary: How chromosome organization is related to genome function remains poorly understood. Cohesin, loop extrusion, and CCCTC-binding factor (CTCF) have been proposed to create topologically associating domains (TADs) to regulate gene expression. Here,
James Rhodes +2 more
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Cohesin mediates DNA loop extrusion by a “swing and clamp” mechanism
Cell, 2021 Summary Structural maintenance of chromosomes (SMC) complexes organize genome topology in all kingdoms of life and have been proposed to perform this function by DNA loop extrusion. How this process works is unknown.
Daniel Canena Manhães de Carvalho +2 more
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The Cohesin Release Factor WAPL Restricts Chromatin Loop Extension
Cell, 2017 Summary The spatial organization of chromosomes influences many nuclear processes including gene expression. The cohesin complex shapes the 3D genome by looping together CTCF sites along chromosomes. We show here that chromatin loop size can be increased
Bas van Steensel +2 more
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Cohesin-dependence of neuronal gene expression relates to chromatin loop length
eLife, 2022 Cohesin and CTCF are major drivers of 3D genome organization, but their role in neurons is still emerging. Here, we show a prominent role for cohesin in the expression of genes that facilitate neuronal maturation and homeostasis.
Lesly Calderon +15 more
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Diverse Roles of Cohesin in Chromosome Dynamics and Stem Cells [PDF]
BioTechThe cohesin complex is a highly conserved, ring-shaped protein assembly that plays fundamental roles in chromosome biology. Originally identified as the molecular glue that holds sister chromatids together from DNA replication until cell division ...
Eui-Hwan Choi
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RAD21 is the core subunit of the cohesin complex involved in directing genome organization
Genome Biology, 2023 Background The ring-shaped cohesin complex is an important factor for the formation of chromatin loops and topologically associating domains (TADs) by loop extrusion.
Yuao Sun +16 more
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Context-dependent regulation of IgH V(D)J recombination by cohesin-STAG1 and cohesin-STAG2 [PDF]
Nature CommunicationsCohesin-mediated loop extrusion is central to IgH V(D)J recombination in early developing B cells, driving D-to-JH rearrangements and VH-to-DJH rearrangements, with the latter step regulated by the cohesin release factor WAPL.
Fujung Chang +7 more
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A Structure-Based Mechanism for DNA Entry into the Cohesin Ring
Molecular Cell, 2020 Despite key roles in sister chromatid cohesion and chromosome organization, the mechanism by which cohesin rings are loaded onto DNA is still unknown.
Juri Rappsilber +2 more
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Cohesin acts as a transcriptional gatekeeper by restraining pause–release to promote processive elongation [PDF]
Nature CommunicationsCohesin organizes 3D chromatin architecture, including promoter–enhancer loops, yet its loss has surprisingly modest effects on steady-state gene expression.
Shoin Tei +7 more
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Interplay between cohesin and TORC1 links chromosome segregation and gene expression to environmental changes [PDF]
eLifeCohesin is a DNA tethering complex essential for chromosome structure and function. In fission yeast, defects in the cohesin loader Mis4 result in chromosome segregation defects and dysregulated expression of genes near chromosome ends.
Dorian Besson +10 more
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