Telomeres stall DNA loop extrusion by condensin
Cell ReportsSummary: DNA loop extrusion by SMC proteins is a key process underlying chromosomal organization. It is unknown how loop extruders interact with telomeres where DNA is densely covered with proteins.
Brian T. Analikwu +14 more
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Condensin loop extrusion properties, roadblocks, and role in homology search during recombination in S. cerevisiae [PDF]
The EMBO JournalThe in vivo mechanism, cis-acting roadblocks, and biological functions of DNA loop extrusion by eukaryotic SMC complexes remain incompletely defined. Here, we identify condensin-dependent Hi-C contact stripes at the recombination enhancer (RE) and at ...
Vinciane Piveteau +6 more
doaj +3 more sources
Chromosome structure in Drosophila is determined by boundary pairing not loop extrusion [PDF]
eLifeTwo different models have been proposed to explain how the endpoints of chromatin looped domains (‘TADs’) in eukaryotic chromosomes are determined.
Xinyang Bing +7 more
doaj +3 more sources
Loop Extrusion Machinery Impairments in Models and Disease [PDF]
CellsStructural maintenance of chromosomes (SMC) complexes play a crucial role in organizing the three-dimensional structure of chromatin, facilitating key processes such as gene regulation, DNA repair, and chromosome segregation.
Anastasiya Ryzhkova +3 more
doaj +4 more sources
Theory of Chromatin Organization Maintained by Active Loop Extrusion [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2023 The active loop extrusion hypothesis proposes that chromatin threads through the cohesin protein complex into progressively larger loops until reaching specific boundary elements. We build upon this hypothesis and develop an analytical theory for active loop extrusion which predicts that loop formation probability is a nonmonotonic function of loop ...
Michael Rubinstein, Brian Chan
exaly +4 more sources
Loop extrusion by cohesin plays a role in enhancer-activated gene expression early in differentiation [PDF]
Nature CommunicationsEnhancers and their target promoters often come into close physical proximity when activated. This may be explained by a variety of mechanisms, including cohesin-mediated chromatin loop extrusion.
Rosa J. Stolper +11 more
doaj +2 more sources
CTCF couples long-range loop extrusion and diffusion to mediate a diverse Igκ repertoire [PDF]
Nature CommunicationsDynamic genome folding is important for V(D)J recombination at the immunoglobulin kappa (Igκ) locus, which recombines Jκ and Vκ gene segments across a 3.2 Mb region in both deletional and inversional orientations.
Emma L. Bush +10 more
doaj +2 more sources
Cohesin distribution alone predicts chromatin organization in yeast via conserved-current loop extrusion [PDF]
Genome BiologyBackground Inhomogeneous patterns of chromatin-chromatin contacts within 10–100-kb-sized regions of the genome are a generic feature of chromatin spatial organization.
Tianyu Yuan +5 more
doaj +2 more sources
CTCF as a boundary factor for cohesin-mediated loop extrusion: evidence for a multi-step mechanism
Nucleus, 2020 Mammalian genome structure is closely linked to function. At the scale of kilobases to megabases, CTCF and cohesin organize the genome into chromatin loops.
Anders S. Hansen
doaj +2 more sources
DNA actively regulates the “safety-belt” dynamics of condensin during loop extrusion [PDF]
Nature CommunicationsCondensin plays an essential role in genome folding through its active DNA loop extrusion activity. Condensin contains a binding interface between its Ycg1 HEAT-repeat subunit and the Brn1 kleisin, together forming a “safety-belt” DNA-binding groove ...
Jinyu Chen +3 more
doaj +2 more sources