The Smc complexes in DNA damage response [PDF]
Cell & Bioscience, 2012 The structural maintenance of chromosomes (Smc) proteins regulate nearly all aspects of chromosome biology and are critical for genomic stability. In eukaryotes, six Smc proteins form three heterodimers--Smc1/3, Smc2/4, and Smc5/6--which together with ...
Wu Nan, Yu Hongtao
doaj +5 more sources
Evidence for binary Smc complexes lacking kite subunits in archaea [PDF]
IUCrJ, 2020 SMC complexes play a central role in chromosome organization in all domains of life. The bacterial Smc–ScpAB complex is a three-subunit complex composed of Smc, ScpA and ScpB.
Jae-Hyun Jeon +6 more
doaj +7 more sources
SMC modulates ParB engagement in segregation complexes in streptomyces [PDF]
Nature CommunicationsParB is a bacterial chromosome segregation protein with recently demonstrated CTPase activity. CTP-bound ParB homodimers are loaded onto DNA at parS sites and spread along DNA, forming a large nucleoprotein complex.
Katarzyna Pawlikiewicz +5 more
doaj +5 more sources
XerD unloads bacterial SMC complexes at the replication terminus [PDF]
Molecular Cell, 2021 Bacillus subtilis structural maintenance of chromosomes (SMC) complexes are topologically loaded at centromeric sites adjacent to the replication origin by the partitioning protein ParB. These ring-shaped ATPases then translocate down the left and right chromosome arms while tethering them together. Here, we show that the site-specific recombinase XerD,
Hugo Brandao, Xindan Wang, Payel Paul
exaly +4 more sources
Structural biology of SMC complexes across the tree of life
Current Opinion in Structural Biology, 2023 Structural maintenance of chromosomes (SMC) complexes guard and organize the three-dimensional structure of chromosomal DNA across the tree of life. Many SMC functions can be explained by an inherent motor activity that extrudes large DNA loops while the complexes move along their substrate.
Jan Lowe, Frank Bürmann
exaly +4 more sources
The SMC Hinge is a Selective Gate for Obstacle Bypass [PDF]
Nature CommunicationsDNA loop-extruding SMC complexes play vital roles in genome maintenance and DNA immunity. However, how these ring-shaped DNA motors navigate large DNA-bound obstacles has remained unclear.
Hon Wing Liu +5 more
doaj +2 more sources
SMC complex unidirectionally translocates DNA by coupling segment capture with an asymmetric kleisin path [PDF]
eLifeSMC (structural maintenance of chromosomes) protein complexes are ring-shaped molecular motors essential for genome folding. Despite recent progress, the detailed molecular mechanism of DNA translocation in concert with the ATP-driven conformational ...
Masataka Yamauchi +3 more
doaj +2 more sources
Single-domain antibody inhibitors target the coiled coil arms of the Bacillus subtilis SMC complex [PDF]
eLifeSynthetic nanobodies—also called sybodies—have proven valuable for stabilizing conformations of purified proteins, advancing structural and functional studies for example of transmembrane proteins. However, their utility in modulating protein function in
Ophélie J Gosselin +4 more
doaj +2 more sources
Replisomes restrict SMC translocation in vivo [PDF]
Nature CommunicationsStructural maintenance of chromosomes (SMC) complexes organize genomes by extruding DNA loops, while replisomes duplicate entire chromosomes. These essential molecular machines must collide frequently in every cell cycle, yet how such collisions are ...
Qin Liao +3 more
doaj +2 more sources
Evolutionary Patterns of the Genes Involved in the Integrity and Segregation of Chromosomes in Sawflies (Hymenoptera: Symphyta) [PDF]
Ecology and EvolutionThe genes involved in the regulation of the meiotic process act important roles in maintaining the structural integrity of chromosomes, ensuring accurate mitotic segregation, and preserving genome stability.
Ayşe Rümeysa Nalça +1 more
doaj +2 more sources