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Nitrocellulose dot-ELISA for serodiagnosis of schistosomiasis
Transactions of the Royal Society of Tropical Medicine and Hygiene, 1987The diagnostic value of the nitrocellulose (NC)-ELISA technique originally described for the detection of viral and bacterial antigens was studied for the detection of antibodies in patients with parasitologically confirmed schistosomiasis, using only about 100 ng total protein content per NC disc of Schistosoma mansoni soluble egg antigen supplied by ...
K, Janitschke, A, Reinhold, L, Bode
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Hamburger meat identification by dot-ELISA
Meat Science, 2000The use of low cost meats to adulterate meats and meat products has been reported. Appropriate methods of analysis then are needed in order to detect this practice. The dot-ELISA method was used to identify the meat of different animal species and to detect adulteration of hamburgers.
A, Macedo-Silva +5 more
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Dot-Elisa for the rapid detection of gentamicin in milk
Journal of Clinical Laboratory Analysis, 1995 AbstractA dipstick dot‐ELISA for the detection of gentamicin in milk of dairy cattle is reported for the first time. The test is based on a sandwich ELISA using high affinity monoclonal antibodies to gentamicin. Antibodies were adsorbed to nitrocellulose filters, blocked, dried, and stored for several weeks before use.
J, Ara, Z, Gans, R, Sweeney, B, Wolf
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Comparison between standard ELISA and dot-ELISA for serodiagnosis of human trichinosis
Transactions of the Royal Society of Tropical Medicine and Hygiene, 1988The dot enzyme-linked immunosorbent assay (dot-ELISA) provided rapid and accurate detection of human trichinosis. Comparable sensitivity and specificity of the standard ELISA and dot-ELISA were obtained when sera of 18 confirmed cases of trichinosis were screened.
S W, Chan, R C, Ko
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Recent applications of the Dot-ELISA in immunoparasitology
Veterinary Parasitology, 1988The dot enzyme-linked immunosorbent assay (Dot-ELISA) is a highly versatile solid-phase immunoassay for antibody or antigen detection. The assay uses minute amounts of reagent dotted onto solid surfaces such as nitrocellulose and other paper membranes which avidly bind proteins. After incubation with antigen-specific antibody and enzyme-conjugated anti-
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The American Journal of Tropical Medicine and Hygiene, 1987
The dot enzyme-linked immunoassay and standard enzyme-linked immunoassay were used to detect Cysticercus cellulosae antigens in cerebrospinal fluid of patients suffering with neurocysticercosis. Using the dot enzyme-linked immunoassay, 10 of 17 patients (59%) were positive at a reciprocal titer of 128 (range 128-1,024). In the standard assay, 13 of 17 (
E, Téllez-Girón +4 more
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The dot enzyme-linked immunoassay and standard enzyme-linked immunoassay were used to detect Cysticercus cellulosae antigens in cerebrospinal fluid of patients suffering with neurocysticercosis. Using the dot enzyme-linked immunoassay, 10 of 17 patients (59%) were positive at a reciprocal titer of 128 (range 128-1,024). In the standard assay, 13 of 17 (
E, Téllez-Girón +4 more
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[Dot-ELISA in the diagnosis of neurocysticercosis].
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 1991The dot-enzyme-linked immunosorbent assay was used to detect Cysticercus cellulosae antibodies in sera of patients with neurocysticercosis. Among 108 confirmed cases of neurocysticercosis 81.6-96.1% showed positive reactions. Two out of 54 normal control sera reacted at a serum dilution of 1:20, but none at a 1:40 (range 40-640).
H J, Jiang +7 more
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Simplification and Standardization of Dot-ELISA for Human Schistosomiasis mansoni
The Journal of Parasitology, 1987Dot-ELISA, a technique that shares the same principles as the enzyme immunoassay, is useful for detection of anti-Schistosoma mansoni antibodies in the sera of patients with Schistosoma mansoni infections. The antigens were fixed to the nitrocellulose strips, blocked with 1% bovine serum albumin in 0.05% Tween 20.
F N, Boctor +3 more
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Dot-ELISA in diagnosis of schistosomiasis.
Journal of the Egyptian Society of Parasitology, 1989One microliter of S. mansoni egg antigen was dotted directly on the nitrocellulose paper sheet acting as the adsorbent surface (9 dots/paper). The sera of 25 Egyptian patients and 15 healthy persons (2 microliters of each) were dotted over the antigen dots, then 2 ml of each of the blocking, washing, HRP-conjugated IgG and DAB adding procedures, were ...
M A, Madwar, M M, Hassan
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Development of a comparative dot ELISA for the detection of antibodies in blastomycosis
Diagnostic Microbiology and Infectious Disease, 1987We present a preliminary study utilizing an enzyme immunoassay for the detection of antibodies in blastomycosis using nitrocellulose membrane as the solid support. The comparative dot enzyme-linked immunosorbent assay (CDE) utilizing Blastomyces dermatitidis, Histoplasma capsulatum, and Coccidioides immitis antigens allows rapid screening of specimens ...
H, Sato, A S, Honigman, G M, Scalarone
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