Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Christie Malry's Own Double-Entry: B.S. Johnson's oppositional discourse of unbelief
Brian Crews
openalex +1 more source
A Molecular and Structural Perspective on Bluetongue Virus Entry and Assembly. [PDF]
Roy P.
europepmc +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Failure characteristics of entry under ultra-close goaf and graded control technology using double I-beam support systems [PDF]
Wenda Wu +6 more
openalex +1 more source
Synthesis of Tronocarpine Framework via a Michael/Lactamization/Michael Cascade Process: Synthesis of Dihydrotronocarpine. [PDF]
Castañón-Garcíaa M +2 more
europepmc +1 more source
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
DEVELOPMENT AND MATCHING OF DOUBLE ENTRY TURBINES FOR THE NEXT GENERATION OF HIGHLY BOOSTED GASOLINE ENGINES [PDF]
Tolga Uhlmanna +8 more
openalex +1 more source
Survival rate and need for maintenance of cover-denture prostheses borne on three or less abutment teeth: ten-year results. [PDF]
Schuster L +3 more
europepmc +1 more source

