Results 41 to 50 of about 79,615 (224)

On the selection for specific genes in doubled haploids [PDF]

, 1992
Doubling haploids from the F1 of a cross between two homozygous lines and selection between the produced homozygous lines is a breeding strategy aimed at producing a population of pure lines quickly and thereby facilitating selection. Different selection
Jansen, R.C., R C Jansen
core   +1 more source

Optimized Cas9‐Enriched Nanopore Sequencing and Analysis Workflow for Clinical Diagnosis of Repeat Expansion Disorders

Advanced Science, EarlyView.
An optimized Cas9‐enriched nanopore sequencing workflow, combined with STRiker, enables simultaneous analysis of disease‐associated STR loci from patient blood. The nCATS–STRiker workflow detects repeat expansions, de novo repeat motifs, interruption patterns, and methylation in a single assay, improving the genetic diagnosis of previously undiagnosed ...
Seungbok Lee, Chanju Jung, Minjeong Kim, Narae Kim, Gue‐Ho Hwang, Soon‐Tae Lee, Kon Chu, Sang Kun Lee, Han‐Joon Kim, Jong‐Hee Chae, Sangsu Bae, Jangsup Moon   +11 more
wiley   +1 more source

Characterization of in vitro haploid and doubled haploid Chrysanthemum morifolium plants via unfertilized ovule culture for phenotypical traits and DNA methylation pattern.

Frontiers in Plant Science, 2014
Chrysanthemum is one of important ornamental species in the world. Its highly heterozygous state complicates molecular analysis, so it is of interest to derive haploid forms.
Haibin eWang, Haibin eWang, Bin eDong, Jiafu eJiang, Weimin eFang, Zhiyong eGuan, Yuan eLiao, Sumei eChen, Fadi eChen, Fadi eChen   +9 more
doaj   +1 more source

Induction of embryogenesis in Brassica rapa L. isolated microspore culture

Биотехнология и селекция растений, 2023
The creation of F1 hybrids combining high productivity, valuable biochemical composition and resistance to biotic and abiotic environmental factors is an urgent area of work with Brassicaceae Burnett.To create a homogeneous, genetically stable source ...
A. A. Aslanova, A. B. Kurina
doaj   +1 more source

A Scalable Droplet Microfluidic Platform That Overcomes Large‐Cell Constraints for Long‐Term Plant Cell Culture and Embryogenesis Analysis

Advanced Science, EarlyView.
This work presents a large‐cell‐compatible droplet microfluidic platform that overcomes long‐standing barriers in plant cell culture by enabling high‐fidelity encapsulation, novel multilayer 3D droplet incubation for scalable long‐term culture, and support for the full developmental progression of large plant cells.
Arman Mirmiran, Fangchi Shao, Gregory Datto, Tathansh Joshi, Elizabeth Chatt, Yashar Bashirzadeh, Dan Stessman, Lai Wei, Kuangwen Hsieh, Hena Guo, Tza‐Huei Wang   +10 more
wiley   +1 more source

DUET‐seq: An Open‐Source Droplet Platform for High‐Fidelity Joint Chromatin and Transcriptome Profiling Reveals Temporal Regulatory Decoupling in Single Cells

Advanced Science, EarlyView.
DUET‐seq is an open‐source droplet platform that jointly profiles chromatin accessibility and gene expression from the same nucleus. Dissolvable dual‐linker hydrogel beads and a one‐step intra‐droplet RT‐PCR co‐index both modalities in under 12 h at roughly $0.04 per cell.
Dong Cheng, Zijun Meng, Lan Wei, Pingjing Yang, Fanfan Zhang, Zhiyi Luo, Ting Lai, Chunli Li, Mengyao Zhao, Mengqin Xu, Jiaqi Wang, Linjun Li, Huarong Chen, Jin Li, Ailong Huang, Youquan Bu, Liuyang Zhao   +16 more
wiley   +1 more source

An Overview of Haploid and Double Haploid Production Methods in Wheat

Black Sea Journal of Agriculture, 2022
For about a century, researchers have been working on haploidy approach. Progressively, they discovered the importance and usefulness of haploids in various research fields. On the other hand, it is suggested that climate change impacts on crop production, especially wheat, requires rapid and efficient methods of plant breeding to produce new cultivars
Noor Maıwan, Mehtap Yıldız, Sana Salih, Sara Luna   +3 more
openaire   +2 more sources

Blue Light Receptor PHR2‐Mediated Spectrum Antiviral Immunity

Advanced Science, EarlyView.
Blue light suppresses RBSDV through the blue‐light receptor ZmPHR2, which interacts with ZmISP in chloroplasts to trigger ROS burst and antiviral defense. The viral effector P6 enhances ZmPHR2–ZmISP interaction, alters their distribution, and suppresses ROS.
Runyi Chen, Yuxin Tai, Xinyu Wang, Chunhua Mu, Xia Liu, Gongjian Li, Jiayi Liu, Xin Lu, Duo Shi, Zhiqiang Zhou, Jienan Han, Zhuanfang Hao, Mingshun Li, Degui Zhang, Hongjun Yong, Zhennan Xu, Jianfeng Weng, Xinhai Li   +17 more
wiley   +1 more source

A Robust Framework for Maize Elite Line Genome Editing Through Enhanced HI-Edit via LbCas12a Activity Optimization. [PDF]

Plant Biotechnol J
ABSTRACT Haploid induction coupled with genome editing (HI‐Edit) enables direct modification of commercial crop varieties, bypassing the need for trait introgression or direct transformation of elite lines with CRISPR machinery. However, its widespread application has been constrained by low haploid editing rates (HER), the proportion of haploids ...
Liang D, Guo H, Wei J, Zhu F, Zhang Y, Green J, Ji Y, Jin H, Zhang X, Gui H, Dan H, Liu Y, Zhang Y, Wang H, Jiang Y, Geng L, Lv J, Cai W, Song W, Kelliher T, Chen X, Egger R.   +21 more
europepmc   +2 more sources

Heat stress in spermatogenesis: a redox‐dependent decision network linking ROS, autophagy, and regulated cell death

Biological Reviews, EarlyView.
ABSTRACT Heat stress (HS) is an increasing threat to male reproductive health, disrupting spermatogenesis through complex and interdependent cellular responses within the seminiferous epithelium. Although excessive reactive oxygen species (ROS) production is a hallmark of HS, ROS also act as central regulators of cell fate decisions beyond their role ...
Ribrio Ivan Tavares Pereira Batista
wiley   +1 more source