Results 31 to 40 of about 1,479 (217)

Anther culture for haploid and doubled haploid production [PDF]

Plant Cell, Tissue and Organ Culture (PCTOC), 2010
Haploids are plants with a gametophytic chromosome number and doubled haploids are haploids that have undergone chromosome duplication. The production of haploids and doubled haploids (DHs) through gametic embryogenesis allows a single-step development of complete homozygous lines from heterozygous parents, shortening the time required to produce ...
openaire   +1 more source

Maize In Planta Haploid Inducer Lines: A Cornerstone for Doubled Haploid Technology [PDF]

, 2021
Doubled haploid (DH) technology produces strictly homozygous fertile plant thanks to doubling the chromosomes of a haploid embryo/seedling. Haploid embryos are derived from either male or female germ line cells and hold only half the number of chromosomes found in somatic plant tissues, albeit in a recombinant form due to meiotic genetic shuffling.
Jacquier, Nathanaël, Gilles, Laurine, Martinant, Jean-Pierre, Rogowsky, Peter, Widiez, Thomas   +4 more
openaire   +4 more sources

Diploidization of cucumber (Cucumis sativus L.) haploids by colchicine treatment

Acta Societatis Botanicorum Poloniae, 2014
Haploid cucumber plants are totally infertile and do not undergo spontaneous diploidization. The use of haploids depends on the possibility of doubling the chromosome number and the obtaining of stable doubled haploids (DH).
Vesselina Nikolova, Katarzyna Niemirowicz-Szczytt   +1 more
doaj   +1 more source

Induction of embryogenesis in Brassica rapa L. isolated microspore culture

Биотехнология и селекция растений, 2023
The creation of F1 hybrids combining high productivity, valuable biochemical composition and resistance to biotic and abiotic environmental factors is an urgent area of work with Brassicaceae Burnett.To create a homogeneous, genetically stable source ...
A. A. Aslanova, A. B. Kurina
doaj   +1 more source

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

Molecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain, Yann Le Page, Frédéric Percevault, Emmanuelle Becker, Luc Negroni, Almudena Fernandez, Marta Cantero, Diego Muñoz‐Santos, Lluís Montoliu, Cyrille Garnier, Michael Primig   +10 more
wiley   +1 more source

Creation of rice doubled haploids with low amylose content using in vitro anther culture [PDF]

Brazilian Journal of Biology
In vitro androgenesis is a unique model for producing homozygous doubled haploid plants. The use of haploid biotechnology accelerates to obtain of doubled haploid plants, which is very important in rice breeding.
B. Usenbekov, A. Amirova, Z. Zeinalov, A. Meldebekova, D. Mynbayeva, Kh. Berkimbay, T. Kurbangaliyeva   +6 more
doaj   +1 more source

DNA Replication Errors Drive Genome‐Wide Small Inverted Triplication Dynamics

Advanced Science, EarlyView.
This study provides insight into the dynamic equilibrium mechanism of a novel structural variant, small inverted triplication (SIT), which is generated by misalignment of the 3’ flap generated under DNA replication stress with palindromic sequence. Alternatively, the end sequence may fold back on itself to form an inverted fragment.
Yi Lei, Yu Zhou, Haitao Sun, Hang Yuan, Xinyu Pei, Jessica D. Hess, Yao Yan, Zunsong Hu, Mian Zhou, Zhaohui Gu, Li Zheng, Xiwei Wu, Binghui Shen   +12 more
wiley   +1 more source

Two Routes to Land: Genomic Underpinnings of Parallel Aerial Egg Deposition in Aquatic Old‐World Pila and New‐World Pomacea (Ampullariidae)

Advanced Science, EarlyView.
Comparative genomics of Gondwana‐diverged Pila and Pomacea reveals parallel evolution of aerial oviposition. Convergent chromosomal rearrangements reshape regulatory landscapes within topologically associating domains. Lineage‐specific gene family expansions and viral‐derived perivitelline proteins (PV1) underpin desiccation resistance.
Yufei Zhou, Huawei Mu, Xueying Nie, Yue Gao, Hui Wang, Ling Fang, Tiangang Luan, Monthon Ganmanee, Jian‐Wen Qiu, Jin Sun, Jack Chi‐Ho Ip   +10 more
wiley   +1 more source

Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selection

Electronic Journal of Biotechnology, 2019
Background: Wheat is one of the most important crops cultivated all over the world. New high-yielding cultivars that are more resistant to fungal diseases have been permanently developed.
Halina Wiśniewska, Maciej Majka, Michał Kwiatek, Magdalena Gawłowska, Maria Surma, Tadeusz Adamski, Zygmunt Kaczmarek, Tadeusz Drzazga, Bogusława Ługowska, Marek Korbas, Jolanta Belter   +10 more
doaj   +1 more source

Metabolic Imbalance Triggers Adaptive Remodeling to Accelerate Diploidization in Murine Haploid Embryonic Stem Cells

Advanced Science, EarlyView.
In this article, Shuai and colleagues demonstrate that metabolic remodeling drives self‐diploidization in murine haploid ESCs (haESCs). Mitochondrial dysfunction and imbalanced pyruvate metabolism underlie this process. Genome‐wide screening using haESCs identifies key mitochondrial quality‐control related genes, enabling a metabolism‐based medium that
Yi Fu, Wenhao Zhang, Yifan Zhang, Yu He, Yi Du, Yiding Zhao, Chunmeng Yao, Shengyi Sun, Xiaoyan Sheng, Qian Gao, Chao Tong, Ling Shuai   +11 more
wiley   +1 more source