Results 51 to 60 of about 6,322 (255)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Active particle in a very thin interfacial droplet

Physical Review Research
A single light-driven Janus particle confined in a very thin oil droplet at an air-water interface displays intriguing dynamics. While laser activation induces rapid horizontal motion (1mm/s–1cm/s) by thermal Marangoni flow, the particle exhibits ...
Airi N. Kato, Kaili Xie, Benjamin Gorin, Jean-Michel Rampnoux, Hamid Kellay   +4 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

The ejection of large non-oscillating droplets from a hydrophobic wedge in microgravity

npj Microgravity, 2021
When confined within containers or conduits, drops and bubbles migrate to regions of minimum energy by the combined effects of surface tension, surface wetting, system geometry, and initial conditions.
Logan J. Torres, Mark M. Weislogel
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Analysis of H-mode and L-mode plasmas with a liquid Sn CPS divertor test unit in the ASDEX Upgrade tungsten divertor

Nuclear Materials and Energy
This study analyzes impurity transport during ASDEX Upgrade experiments in which a Liquid Tin Module (LTM) was exposed to diverted plasmas. Using ASTRA and Aurora, we interpret H-mode and L-mode discharges to assess tin penetration into the core and its ...
Elisabetta Bray, Daniel Fajardo, Clemente Angioni, Oleg Samoylov, Ralph Dux, Giuseppe F. Nallo, Chiara Marchetto, Fabio Subba   +7 more
doaj   +1 more source

Droplet Tracking for Single Molecule Confinement [PDF]

Biophysical Journal, 2011
We report on a robust system for tracking femtoliter and smaller aqueous droplets in oil over tens of minutes with a few nanometers accuracy in a 150 Hz bandwidth. Tracking permits the droplet position to be maintained in the detection volume of a confocal microscope without need for physical or optical trapping, permitting detailed optical analysis of
Gamari, Benjamin D., Arsenault, Mark E., Milas, Peker, Buckman, Richard E., Wilhelm, Christophe, Goldner, Lori S.   +5 more
openaire   +1 more source

Dimethyl Fumarate, But Not Rituximab, Reduces Serum GFAP Levels and PIRMA in Relapsing–Remitting MS

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Serum neurofilament light chain (sNfL) and glial fibrillary acidic protein (sGFAP) levels are believed to reflect mainly acute and chronic disease processes in multiple sclerosis (MS), respectively. In this study, we investigated whether dimethyl fumarate (DMF) and rituximab (RTX) differentially affect these biomarkers.
F. Shawket, J. Lycke, J. Salzer, F. Piehl, K. Fink, N. Lange, J. Mellergård, C. Malmeström, P. Sundström, I. Erngren, A. al‐Grety, E. Freyhult, K. Kultima, J. Burman, A. Svenningsson   +14 more
wiley   +1 more source

Mechanics and stability of vesicles and droplets in confined spaces

Physical Review E, 2016
The permeation and trapping of soft colloidal particles in the confined space of porous media are of critical importance in cell migration studies, design of drug delivery vehicles, and colloid separation devices. Our current understanding of these processes is however limited by the lack of quantitative models that can relate how the elasticity, size,
Eduard, Benet, Franck J, Vernerey
openaire   +3 more sources

Droplet–turbulence interaction in a confined polydispersed spray: effect of turbulence on droplet dispersion [PDF]

Journal of Fluid Mechanics, 2016
The effect of entrained air turbulence on dispersion of droplets (with Stokes number based on the Kolmogorov time scale,$St_{{\it\eta}}$, of the order of 1) in a polydispersed spray is experimentally studied through simultaneous and planar measurements of droplet size, velocity and gas flow velocity (Hardalupaset al.,Exp. Fluids, vol. 49, 2010, pp. 417–
Sahu, S., Hardalupas, Y., Taylor, A. M. K. P.   +2 more
openaire   +2 more sources