Results 51 to 60 of about 221,845 (303)

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Temporal Behavior of the Individual Soft Microparticles: Understanding the Detection by Particle Impact Electrochemistry [PDF]

, 2020
Emerging progress of the Particle Impact Electrochemistry (PIE) technique has opened a novel field of detection and characterization of many analyte particles.
Ahmed, Junaid, Alvarez, Julio C
core   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Development and Characterization of Functional O/W Emulsions with Chia Seed (Salvia hispanica L.) by-Products

Proceedings, 2020
Physicochemical properties of O/W emulsions containing functional ingredients (high ω-3 fatty acid content, protein, and soluble fiber) from chia seeds with different protein–carbohydrate combinations (sodium caseinate-lactose, sodium caseinate ...
Luciana M. Julio, Vanesa Y. Ixtaina, Mabel C. Tomás   +2 more
doaj   +1 more source

Aggressive prostate cancer is associated with pericyte dysfunction

Molecular Oncology, EarlyView.
Tumor‐produced TGF‐β drives pericyte dysfunction in prostate cancer. This dysfunction is characterized by downregulation of some canonical pericyte markers (i.e., DES, CSPG4, and ACTA2) while maintaining the expression of others (i.e., PDGFRB, NOTCH3, and RGS5).
Anabel Martinez‐Romero, Ane Martinez‐Larrinaga, Joaquim Grego‐Bessa, Saioa Garcia‐Longarte, Hielke van Splunder, Ianire Astobiza, Amaia Ercilla, Laura Bozal‐Basterra, Isabel Mendizabal, Pilar Villacampa, Arkaitz Carracedo, Mariona Graupera   +11 more
wiley   +1 more source

Self-organized criticality driven by droplet influx and random fusion

Physical Review Research
The droplet size distribution typically decays exponentially in solutions formed by liquid-liquid phase separation. Nevertheless, a power-law distribution of nucleolus volumes has been observed in amphibian oocytes, which appears similar to the cluster ...
Bohan Lyu, Jie Lin
doaj   +1 more source

Characteristics of Spot Spraying and Continuous Spraying Systems

Agriculture
This paper studied the atomization characteristics of different spray nozzles under the spot spraying method and designed a test system for the atomization characteristics.
Xueguan Zhao, Zhanwei Ma, Chunfeng Zhang, Zhichong Wang, Jing Chen, Xinwei Zhang, Changyuan Zhai   +6 more
doaj   +1 more source

Generalisation of Levine's prediction for the distribution of freezing temperatures of droplets: A general singular model for ice nucleation [PDF]

, 2013
Models without an explicit time dependence, called singular models, are widely used for fitting the distribution of temperatures at which water droplets freeze. In 1950 Levine developed the original singular model.
Sear, Richard P.
core   +3 more sources

Detection of circulating tumor DNA in colorectal cancer patients using a methylation‐specific droplet digital PCR multiplex

Molecular Oncology, EarlyView.
We developed a cost‐effective methylation‐specific droplet digital PCR multiplex assay containing tissue‐conserved and tumor‐specific methylation markers. The assay can detect circulating tumor DNA with high accuracy in patients with localized and metastatic colorectal cancer.
Luisa Matos do Canto, Louise Raunkilde, Jan Lindebjerg, Mads Malik Aagaard, Christina Therkildsen, Jakob Kleif, Lars Henrik Jensen, Torben Frøstrup Hansen, Rikke Fredslund Andersen   +8 more
wiley   +1 more source

Droplet size distribution measurement of water in kerosene by the buoyancy weighing-bar method

MATEC Web of Conferences, 2018
The buoyancy weighing-bar method (BWM) is a method that requires a simple apparatus set up yet produce the high accuracy result in particle size distribution measurement both settling and floating particles.
Tambun Rondang, Sibagariang Piety, Alphantaria Brigitta, Rambe Ahmad Mulia   +3 more
doaj   +1 more source