Results 91 to 100 of about 2,380,969 (281)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Numerical Investigation of the Main Characteristics of Heat and Mass Transfer while Heating the Heterogeneous Water Droplet in the Hot Gases

EPJ Web of Conferences, 2016
The processes of heat and evaporation of heterogeneous water droplet with solid (by the example of carbon) inclusion in hot (from 800 K to 1500 K) gases were investigated by the developed models of heat and mass transfer.
Piskunov Maxim V., Shcherbinina Anastasia A., Vysokomornaya Olga V.   +2 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Numerical study on the thermodynamic behavior of de-icing liquid droplets impacting walls

Advances in Aerodynamics
Spraying de-icing fluid is a key method to ensure the safe operation of aircraft in icy and snowy weather. The film aggregation and internal mixing of de-icing fluid droplets on the aircraft skin during a collision are crucial.
Jing Cui, Shuxin Niu, Guangfeng Yang
doaj   +1 more source

Investigation of Regularities of Heat and Mass Transfer and Phase Transitions during Water Droplets Motion through High-Temperature Gases

Advances in Mechanical Engineering, 2014
The macroscopic regularities of heat and mass transfer and phase transitions during water droplets motion through high-temperature (more than 1000 K) gases have been investigated numerically and experimentally.
Roman S. Volkov, Olga V. Vysokomornaya, Genii V. Kuznetsov, Pavel A. Strizhak   +3 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

High-Throughput Metal 3D Printing Pen Enabled by a Continuous Molten Droplet Transfer. [PDF]

Adv Sci (Weinh), 2023
Kim CK, Cho DW, Kim S, Song SW, Seo KM, Cho YT.   +5 more
europepmc   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Mathematical Modeling And Numerical Simulation of Dropwise Condensation on an Inclined Circular Tube

Journal of Aerospace Technology and Management, 2017
Dropwise condensation can improve heat transfer process and, consequently, leads to considerable reduction in size and weight of condensers as well as improvement in the dehumidification process in many applications, especially in civil transport ...
Hamid Reza Talesh Bahrami, Hamid Saffari
doaj  

Heat and Mass Transfer of Droplets in a Gas Environment

Environmental and Climate Technologies
A numerical simulation of the evaporation of a water droplet in dry air and superheated steam was performed, both with and without consideration of convective heat transfer in the dispersed liquid phase.
Pavlenko Anatoliy
doaj   +1 more source