Results 21 to 30 of about 407,893 (313)

Establishment of Drosophila intestinal cell lines as tools for multiomic screening and deciphering intestinal biology

Scientific Reports
The Drosophila intestine is a powerful model for stem cell dynamics and epithelial biology, yet no intestinal derived cell lines have been available until now. Here, we describe the establishment of Drosophila intestinal cell lines.
Arthur Luhur, Daniel Mariyappa, Peter Bohall, Laura Multini, Morgan Elkins, Andrew C. Zelhof   +5 more
doaj   +1 more source

Pooled genome-wide CRISPR activation screening for rapamycin resistance genes in Drosophila cells

eLife, 2023
Loss-of-function and gain-of-function genetic perturbations provide valuable insights into gene function. In Drosophila cells, while genome-wide loss-of-function screens have been extensively used to reveal mechanisms of a variety of biological processes,
Baolong Xia, Raghuvir Viswanatha, Yanhui Hu, Stephanie E Mohr, Norbert Perrimon   +4 more
doaj   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

A study on the antioxidant, cytotoxicity, and coagulation potential of carbon quantum dots derived from the leaves of Lagerstroemia speciosa

Hybrid Advances
The green synthesis of carbon quantum dots (CQDs) from natural sources offers a sustainable approach in nanotechnology. In this study, CQDs were synthesized from Lagerstroemia speciosa leaves (LS-CQDs) and extensively characterized for their optical ...
Sapam Riches Singh, Gouramma R. Kanamadi, Bothe Thokchom, Santosh Mallikarjun Bhavi, Megha B. Abbigeri, Pooja Joshi, Sudheendra Rao Kulkarni, Akshata Choudhari Padti, B.P. Harini, Ramesh Babu Yarajarla   +9 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

A novel insertional allele of the CG18135 gene is associated with severe mutant phenotypes in Drosophila melanogaster

Frontiers in Genetics
Drosophila melanogaster has been at the forefront of genetic studies and biochemical modeling for over a century. Yet, the functions of many genes are still unknown, mainly because no phenotypic data are available.
Attila Cristian Ratiu, Attila Cristian Ratiu, Adrian Ionascu, Adrian Ionascu, Alexandru Al. Ecovoiu   +4 more
doaj   +1 more source

Molecular bases of circadian magnesium rhythms across eukaryotes

FEBS Letters, EarlyView.
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley   +1 more source

qDATA - an R application implementing a practical framework for analyzing quantitative real-time PCR data

BioTechniques
Gene expression assays that are based on quantitative real-time PCR (qRT-PCR) method are still very popular, therefore, we developed qDATA, an open-source R-based bioinformatics application that offers a quick and intuitive analysis of raw cycle ...
Adrian Ionascu, Alexandru Al. Ecovoiu, Mariana Carmen Chifiriuc, Attila Cristian Ratiu   +3 more
doaj   +1 more source

Effect of Itrifal Muqawwi-e-Dimagh (a polyherbal drug) on the transgenic Drosophila model of Parkinson's Disease

Phytomedicine Plus, 2021
Background: Parkinson's Disease (PD) is characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta of the mid brain which leads to the depletion of dopamine levels.
Yasir Hasan Siddique, Falaq Naz, Rahul, Mohammad Rashid, Shariq Mian   +4 more
doaj   +1 more source