Results 71 to 80 of about 139,709 (220)

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Identification and green synthesis of potato dry rot Fusarium falciforme silver nanoparticles, characterization and antibacterial activity

BMC Plant Biology
Potato tubers collected from different areas showed the prevalence of dry rot with characteristic white mycelia like symptoms of Fusarium pathogen. Fusarium species from diseased tubers were isolated based on morphological features.
Syed Haseeb Shah, Xiaoliang Shan, Sofia Baig, Hongwei Zhao, Maria Siddique, Bushra Ismail, Irum Shahzadi, Muhammad Saqib Bilal, Shamyla Nawazish, Ishrat Naz, Ayesha Baig   +10 more
doaj   +1 more source

Hydrate technology for water desalination in the Mekong Delta, Vietnam

Heliyon
Freshwater scarcity is a critical issue in Vietnam, particularly in the Mekong Delta, a densely populated region with an agriculture-based economy. This scarcity is largely driven by saltwater intrusion during the dry season, severely affecting both ...
Quang Nhat Tran, Nhi Ngoc Thi Vo, Thao Thi Pham, Hai Son Truong-Lam   +3 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Eco-friendly water-synthesis of lead-free Cs2AgBiBr6 perovskites

Communications Materials
The synthesis of lead-free Cs2AgBiBr6 perovskite is a huge challenge within the framework of the new emergent full-inorganic materials, with the aim of being employed as sensitive layers for a new generation of solar cells and/or optoelectronic devices ...
Paola De Padova, Bruno Olivieri, Carlo Ottaviani, Osamu Kubo, Sofia Caretto, Angelo De Paolis, Annalisa Paradiso, Paola Prete, Nico Lovergine, Filippo Ruggiero, Amanda Generosi, Barbara Paci, Rodrigo Szostak, Francisco Mateus Cirillo da Silva, Hélio C. N. Tolentino, Tulio Costa Rizuti da Rocha, Luiz Gustavo de Freitas Borges, Flavio Cesar Vicentin, Aldo Di Carlo   +18 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Morphology and conductivity control in hexagonal ZnO nanorods through annealing for gas sensor applications

Физико-химические аспекты изучения кластеров, наноструктур и наноматериалов
This study investigates the influence of the annealing temperature (300-500°C) on the electrophysical properties of nanostructured zinc oxide (ZnO) layers synthesized by a sol–gel method. The obtained samples consist of an ensemble of hexagonal nanorods.
N.A. Klychkov, V.V. Simakov, I.V. Sinev, A.M. Zakharevich, V.V. Efanova   +4 more
doaj   +1 more source