Results 191 to 200 of about 384,390 (264)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Combining forces in dual certified centers of geriatric trauma/joint replacement according to the German trauma society (DGU)/endocert improves mobility and quality of life in elderly patients with femoral neck fractures: an analysis from the registry for geriatric trauma (ATR-DGU). [PDF]

Eur J Trauma Emerg Surg
Lampert C, Schmidt H, Zhang Y, Gleich J, Fleischhacker E, Schoeneberg C, Pass B, Bormann M, Arnholdt J, Holzapfel BM, Böcker W, Lerchenberger M, Neuerburg C, Working Committee on Geriatric Trauma Registry (AK ATR) of the German Trauma Society.   +13 more
europepmc   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Response to the Letter to Editor regarding: "High effectiveness of multilevel orthopedic surgery and guided growth in spastic hemiplegia children". [PDF]

J Child Orthop
Mamedov U, Dolganova T, Gatamov O, Foster P, Tomov A, Popkov D.   +5 more
europepmc   +1 more source

Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe

FEBS Open Bio, EarlyView.
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley   +1 more source

Construction of remote dual stereocenters by electrochemical cobalt-catalyzed enantioselective desymmetrization. [PDF]

Nat Commun
Li Y, Liu S, Yuan B, Graw N, Ackermann L.   +4 more
europepmc   +1 more source

TMC4 localizes to multiple taste cell types in the mouse taste papillae

FEBS Open Bio, EarlyView.
Transmembrane channel‐like 4 (TMC4), a voltage‐dependent chloride channel, plays a critical role in amiloride‐insensitive salty taste transduction. TMC4 is broadly expressed in all mature taste cell types, suggesting a possible involvement of multiple cell types in this pathway.
Momo Murata, Yoshikazu Saito, Yoichi Kasahara, Wataru Fujii, Takumi Misaka, Tomiko Asakura, Masataka Narukawa   +6 more
wiley   +1 more source

Multispectral PCCT and CBCT imaging for high precision radiotherapy through translation of imaging parameters with machine learning validation. [PDF]

Sci Rep
Dreher C, Vellala A, Siefert V, Haag F, Sawall S, Fleckenstein J, Clausen S, Boda-Heggemann J, Schoenberg SO, Giordano FA, Froelich M.   +10 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source