Results 111 to 120 of about 3,875,475 (226)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells

FEBS Open Bio, EarlyView.
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai, Yong Xu, Jingjing Ma, Ratchanee Duangrat, Chen Yan, Jingyan Liang, Hua Wei, Bin Jiang, Tao‐Sheng Li   +8 more
wiley   +1 more source

Association between frailty and dual sensory loss: hearing and vision

, 2021
Dual sensory loss (DSL) refers to the presence of both hearing- and vision- loss. The objective of this study was to explore the link between frailty and DSL in older adults. This cross-sectional study was conducted in a public health rehabilitation center with one hundred seven older adults Participants with hearing loss had significantly higher ...
Garcia, Ana Carla Oliveira, Wittich, Walter, Momensohn-Santos, Teresa   +2 more
openaire   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Survey on Rules on Loss of Nationality in International Treaties and Case Law. CEPS Paper in Liberty and Security in Europe No. 57, 30 August 2013 [PDF]

, 2013
This paper offers a picture of the obligations existing under international and European law in respect of the loss of nationality. It describes international instruments including obligations in this field with direct relevancy for the loss of ...
de Groot, René
core  

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Sensory impairments and caregiver burden: a population-based study of older adults in Thailand

BMC Geriatrics
Background Visual and hearing impairments can increase dependence across the life course; however, their impact may be particularly pronounced in older adults due to age-related functional decline and increased care needs.
Patama Vapattanawong, Ruttana Phetsitong
doaj   +1 more source

Breaking methodological boundaries: Assistive technology development involving people with deafblindness

Nordisk Välfärdsforskning
Background: Deafblindness presents multifaceted challenges in communication, access to information, and independent mobility. Although assistive technologies can enhance safety, independence, and social participation, most are designed for single sensory
Kari Jokstad, Bedir Yiyit, Erik Nilsen, Fereshteh Halimi, Hilde Thygesen, Marie Dyrkorn Egge, Emilio Jensen Granados   +6 more
doaj   +1 more source

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

FEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill, Hassan Yousef Othman AlMuallim, Elouisa Maddock, Carl Hobbs, Earl Clarke, Maria B. Goncalves, Jonathan P. T. Corcoran   +6 more
wiley   +1 more source

Association of hearing, vision, and dual sensory impairment and risk of Alzheimer’s disease: a nested case-control study

BMC Geriatrics
Background Hearing impairment is a known risk factor for Alzheimer’s disease (AD), although less is known about vision impairment or dual sensory impairment (DSI) as risk factors for AD.
Blair Rajamaki, Kaisa Hokkinen, Aarno Dietz, Kai Kaarniranta, Sirpa Hartikainen, Anna-Maija Tolppanen   +5 more
doaj   +1 more source