Results 71 to 80 of about 471 (209)

Dynamics of Open DNA Sliding Clamps

PLOS ONE, 2016
A range of enzymes in DNA replication and repair bind to DNA-clamps: torus-shaped proteins that encircle double-stranded DNA and act as mobile tethers. Clamps from viruses (such as gp45 from the T4 bacteriophage) and eukaryotes (PCNAs) are homotrimers, each protomer containing two repeats of the DNA-clamp motif, while bacterial clamps (pol III β) are ...
openaire   +5 more sources

Solution study of the Escherichia coli DNA polymerase III clamp loader reveals the location of the dynamic ψχ heterodimer

Structural Dynamics, 2015
Several X-ray crystal structures of the E. coli core clamp loader containing the five core (δ′, δ, and three truncated γ) subunits have been determined, but they lack the ψ and χ subunits.
Farzaneh Tondnevis, Richard E. Gillilan, Linda B. Bloom, Robert McKenna   +3 more
doaj   +1 more source

cryo-EM structures of the E. coli replicative DNA polymerase reveal its dynamic interactions with the DNA sliding clamp, exonuclease and τ

eLife, 2015
The replicative DNA polymerase PolIIIα from Escherichia coli is a uniquely fast and processive enzyme. For its activity it relies on the DNA sliding clamp β, the proofreading exonuclease ε and the C-terminal domain of the clamp loader subunit τ.
Rafael Fernandez-Leiro, Julian Conrad, Sjors HW Scheres, Meindert H Lamers   +3 more
doaj   +1 more source

Cellular Responses to Mechanical Cues Across Scales: From Fundamental Insights to Translational Potential

Advanced Healthcare Materials, EarlyView.
This review examines how cellular behavior is regulated by mechanical cues transmitted through soft biomaterials, from single‐cell mechanosensing to tissue‐level adaptation. It highlights why physiological relevance, rather than model complexity alone, is critical for translational mechanobiology and introduces a scoring framework linking material ...
Mathias Polz, Manuel P. Kainz, Niklas Haider, Theresa Rienmüller, Kerstin Lenk, Rupert Kargl, Michele Terzano, Gerhard Sommer, Gerhard A. Holzapfel, Julia Fuchs   +9 more
wiley   +1 more source

Multifunctional Catechol‐Functionalized Cellulose Hydrogels for the Minimally Invasive Treatment of Acute Optic Nerve Injuries

Advanced Healthcare Materials, EarlyView.
Catechol‐functionalized cellulose hydrogels are developed as injectable, bioadhesive platforms for retinal neuroprotection. The hydrogels exhibit tunable rheological and mechanical properties, strong tissue adhesion, and sustained antioxidative activity.
Kai‐Hsiang Chang, Yi‐Ke Lin, Wei‐Li Chen, Ta‐Ching Chen, Jiashing Yu   +4 more
wiley   +1 more source

Dynamic Factors for Transmitter Release at Small Presynaptic Boutons Revealed by Direct Patch-Clamp Recordings

Frontiers in Cellular Neuroscience, 2019
Small size of an axon and presynaptic structures have hindered direct functional analysis of axonal signaling and transmitter release at presynaptic boutons in the central nervous system.
Shin-ya Kawaguchi, Shin-ya Kawaguchi, Shin-ya Kawaguchi   +2 more
doaj   +1 more source

Plasmonic Enhancement of Fluorescence and Protein Dynamics in Living Mammalian Cells

Advanced Materials, EarlyView.
This study demonstrates plasmonic enhancement of the function of fluorescent voltage sensing proteins (genetically encoded voltage indicators, (GEVIs), QuasAr6) in live mammalian cells. Coupling to plasmonic nanoparticles does not just increase fluorescence, but influences the protein photocycle, creating a hybrid sensor with its response speed to ...
Marco Locarno, Qiangrui Dong, Marco Post, Xin Meng, Cristiano Glessi, Nynke Marije Hettema, Nidas Brandsma, Sebbe Blokhuizen, Alejandro Castañeda Garcia, Srividya Ganapathy, Thieme Schmidt, Lars van Roemburg, Bing Xu, Chun‐Ting Cho, Liedewij Laan, Miao‐Ping Chien, Daan Brinks   +16 more
wiley   +1 more source

Flexible Dielectric Acoustic Resonator Patch for Tissue Regeneration

Advanced Materials, EarlyView.
A flexible dielectric acoustic resonator patch enables MHz‐range ultrasound generation through resonance amplification without using piezoelectric materials. Conformal integration on a curved substrate allows efficient acoustic delivery to tissue‐mimicking environments.
Donyoung Kang, Byeongseok Ryu, Sujeong Ahn, Junhyub Kim, Myeonghun Jang, Jeong Ho Cho, Won‐Gun Koh, Hyungsuk Lee   +7 more
wiley   +1 more source

Topology and Material Optimization in Ultra‐Soft Magneto‐Active Structures: Making Advantage of Residual Anisotropies

Advanced Materials, EarlyView.
Residual magnetization induces pronounced mechanical anisotropy in ultra‐soft magnetorheological elastomers, shaping deformation and actuation even without external magnetic fields. This study introduces a computational‐experimental framework integrating magneto‐mechanical coupling into topology optimization for designing soft magnetic actuators with ...
Carlos Perez‐Garcia, Rogelio Ortigosa, Jesús Martínez‐Frutos, Daniel Garcia‐Gonzalez   +3 more
wiley   +1 more source

Rational Tuning of Hygroscopic Oscillation of Stacked Nanoflake Assemblies for Continuous Ambient Energy Harvesting

Advanced Materials, EarlyView.
Stacked nanoflake assembly (SNA) membranes can oscillate autonomously, offering opportunities for soft actuation and energy harvesting. This work uncovers the physical mechanism behind the sustained oscillation of SNA membranes in gradient humidity and identifies three governing dimensionless parameters, enabling rational design for optimizing SNA ...
Zijing Zhang, Jimin Fu, Gesa Zhang, Zhihua Liang, Boxun Zhang, Haimin Yao   +5 more
wiley   +1 more source