Results 11 to 20 of about 477 (209)

Dynamic clamp with StdpC software [PDF]

Nature Protocols, 2011
Dynamic clamp is a powerful method that allows the introduction of artificial electrical components into target cells to simulate ionic conductances and synaptic inputs. This method is based on a fast cycle of measuring the membrane potential of a cell, calculating the current of a desired simulated component using an appropriate model and injecting ...
Kemenes, Ildikó, Marra, Vincenzo, Crossley, Michael, Samu, Dávid, Staras, Kevin, Kemenes, György, Nowotny, Thomas   +6 more
openaire   +3 more sources

Clusters of cooperative ion channels enable a membrane-potential-based mechanism for short-term memory

eLife, 2020
Across biological systems, cooperativity between proteins enables fast actions, supra-linear responses, and long-lasting molecular switches. In the nervous system, however, the function of cooperative interactions between voltage-dependent ionic channels
Paul Pfeiffer, Alexei V Egorov, Franziska Lorenz, Jan-Hendrik Schleimer, Andreas Draguhn, Susanne Schreiber   +5 more
doaj   +1 more source

Sodium channels implement a molecular leaky integrator that detects action potentials and regulates neuronal firing

eLife, 2020
Voltage-gated sodium channels play a critical role in cellular excitability, amplifying small membrane depolarizations into action potentials. Interactions with auxiliary subunits and other factors modify the intrinsic kinetic mechanism to result in new ...
Marco A Navarro, Autoosa Salari, Jenna L Lin, Luke M Cowan, Nicholas J Penington, Mirela Milescu, Lorin S Milescu   +6 more
doaj   +1 more source

Loading Dynamics of a Sliding DNA Clamp [PDF]

Angewandte Chemie, 2014
AbstractSliding DNA clamps are loaded at a ss/dsDNA junction by a clamp loader that depends on ATP binding for clamp opening. Sequential ATP hydrolysis results in closure of the clamp so that it completely encircles and diffuses on dsDNA. We followed events during loading of an E. coli β clamp in real time by using single‐molecule FRET (smFRET).
Cho, Won-Ki, Jergic, Slobodan, Kim, Daehyung, Dixon, Nicholas, Lee, Jong-Bong   +4 more
openaire   +5 more sources

A dynamic clamp protocol to artificially modify cell capacitance

eLife, 2022
Dynamics of excitable cells and networks depend on the membrane time constant, set by membrane resistance and capacitance. Whereas pharmacological and genetic manipulations of ionic conductances of excitable membranes are routine in electrophysiology ...
Paul Pfeiffer, Federico José Barreda Tomás, Jiameng Wu, Jan-Hendrik Schleimer, Imre Vida, Susanne Schreiber   +5 more
doaj   +1 more source

Applications of Dynamic Clamp to Cardiac Arrhythmia Research: Role in Drug Target Discovery and Safety Pharmacology Testing

Frontiers in Physiology, 2018
Dynamic clamp, a hybrid-computational-experimental technique that has been used to elucidate ionic mechanisms underlying cardiac electrophysiology, is emerging as a promising tool in the discovery of potential anti-arrhythmic targets and in ...
Francis A. Ortega, Eleonora Grandi, Trine Krogh-Madsen, David J. Christini, David J. Christini   +4 more
doaj   +1 more source

Rapid genetic algorithm optimization of a mouse computational model: Benefits for anthropomorphization of neonatal mouse cardiomyocytes

Frontiers in Physiology, 2012
While the mouse presents an invaluable experimental model organism in biology, its usefulness in cardiac arrhythmia research is limited in some aspects due to major electrophysiological differences between murine and human action potentials (APs).
Corina Teodora Bot, Armen R. Kherlopian, Armen R. Kherlopian, Francis A. Ortega, David J. Christini, David J. Christini, Trine eKrogh-Madsen, Trine eKrogh-Madsen, Trine eKrogh-Madsen   +8 more
doaj   +1 more source

Hidden synaptic differences in a neural circuit underlie differential behavioral susceptibility to a neural injury

eLife, 2014
Individuals vary in their responses to stroke and trauma, hampering predictions of outcomes. One reason might be that neural circuits contain hidden variability that becomes relevant only when those individuals are challenged by injury.
Akira Sakurai, Arianna N Tamvacakis, Paul S Katz   +2 more
doaj   +1 more source

Injection of IK1 through dynamic clamp can make all the difference in patch-clamp studies on hiPSC-derived cardiomyocytes

Frontiers in Physiology, 2023
Human-induced stem cell-derived cardiomyocytes (hiPSC-CMs) are a valuable tool for studying development, pharmacology, and (inherited) arrhythmias. Unfortunately, hiPSC-CMs are depolarized and spontaneously active, even the working cardiomyocyte subtypes
Arie O. Verkerk, Arie O. Verkerk, Ronald Wilders   +2 more
doaj   +1 more source

Pacemaker neuron and network oscillations depend on a neuromodulator-regulated linear current

Frontiers in Behavioral Neuroscience, 2010
Linear leak currents have been implicated in the regulation of neuronal excitability, generation of neuronal and network oscillations, and network state transitions.
Shunbing Zhao, Jorge P Golowasch, Jorge P Golowasch, Farzan Nadim, Farzan Nadim   +4 more
doaj   +1 more source