Results 121 to 130 of about 19,086,752 (365)

Homologous expression and purification of human HAX‐1 for structural studies

open access: yesFEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Research on the Model of E-commerce of China’s Urban Informatization Community

open access: yesSHS Web of Conferences, 2014
Urban informatization e-commerce is a business model of the combination of e-commerce operators and organizational forms of community property management, and the import of people management and property management into e-commerce.
Yu Han, Hongyun Li, Yunxia Ning, Yu Hou
doaj   +1 more source

Analysis of performance measures to handle medical E-commerce shopping cart abandonment in cloud

open access: yesInformatics in Medicine Unlocked, 2017
The E-commerce zone is crowded with many Internet users. Medical E-commerce has had significant growth in part because of a great deal of growth in the Indian E-commerce field.
Vedhanayagam Priya   +2 more
doaj   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

open access: yesFEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul   +3 more
wiley   +1 more source

Positive vs. Negative E-WOM: Understanding the More Significant Role in Mediating E-Commerce Quality on Online Impulse Buying

open access: yesJurnal Aplikasi Manajemen
Electronic Word-of-Mouth (e-WOM) can convey product information about ratings, desires, requests and experiences. Therefore, it plays an important role in influencing purchases.
Rivaldi Arissaputra   +5 more
doaj   +1 more source

Optimization of delivery for an e-commerce industry using Six Sigma methodology [PDF]

open access: gold, 2023
Sumit Shandilya   +3 more
openalex   +1 more source

The photoswitchable cannabinoid azo‐HU308 enables optical control of Ca2+ dynamics in INS‐1 β‐cells via off‐target effects on TRPC channels

open access: yesFEBS Open Bio, EarlyView.
Light activation of the photoswitchable cannabinoid ligand azo‐HU308 triggers Ca2+ influx in pancreatic β‐cells through TRPC channels, independent of CB2 cannabinoid receptors. This reveals a non‐GPCR pathway for cannabinoid modulation of β‐cell Ca2+ dynamics and establishes azo‐HU308 as an optical tool to study cannabinoid signaling through TRP ...
Alexander E. G. Viray, James A. Frank
wiley   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

open access: yesFEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq   +3 more
wiley   +1 more source

Integrating customer relationship management strategies in (B2C) e-commerce environments

open access: yes, 2003
Creating value and generating a total customer experience(TCE ) is important for E -Commerce in order to attract customers. However, with increasing competition in the marketplace, it is becoming increasingly difficult to retain customers.
Dawson, Liisa   +2 more
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