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Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Background Fibroblasts, especially cancer-associated fibroblasts (CAFs), represent the predominant stromal cell population in the tumor microenvironment and have an important function in tumorigenesis by interacting with tumor cells.
Wei Cheng +7 more
doaj +1 more source
Appendix E1 The Acquisition Parameters and Retrieving Procedure Appendix E2 MR Imaging Preparation for Radiomic Analysis Appendix E3 MR Imaging Interpretation Appendix E4 Computer code used to compute the Radiomics Features Appendix E5 Categorical ...
Hwan-ho Cho (7048214) +8 more
core +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
ANALYSIS OF SOCIO-ECONOMIC IMPACT OF MIGRATION [PDF]
The expansion of the boundaries of globalization in itself leads to the facilitation of the movement of people from one country to another. As the reality of past decades has shown, this could be voluntary or involuntary movement, which is caused by the ...
Genadi Iashvili, Ana Baratashvili
doaj +1 more source
E1 levels and temporal pattern of expression are significantly altered in E1+/+E2−/− spermatocytes.
(A) (a–e) Localization of cyclin E2 (green) and SYCP3 (red) during prophase I in E1−/−E2+/+ spermatocytes. Insets magnify the sex chromosomes (XY) present in c–e.
Marcia Manterola (260674) +8 more
core +1 more source
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar +11 more
wiley +1 more source
The Muslim Council of Britain: Progressive Interlocutor or Redundant Gatekeeper?
Since its inception in 1997, the Muslim Council of Britain (MCB) has evolved to become one of the most enduring British Muslim organisations. It is a representative body for over 500 member bodies (‘affiliates’) including mosques, schools and charities ...
Harun Khan, Hassan Joudi, Zahraa Ahmed
doaj +1 more source
Diurnal Expression Pattern, Allelic Variation, and Association Analysis Reveal Functional Features of the E1 Gene in Control of Photoperiodic Flowering in Soybean. [PDF]
Although four maturity genes, E1 to E4, in soybean have been successfully cloned, their functional mechanisms and the regulatory network of photoperiodic flowering remain to be elucidated. In this study, we investigated how the diurnal expression pattern
Hong Zhai +10 more
doaj +1 more source
Summary of immunoprecipitation (IP) and E1’ or p62-E1 ELISA resultsa.
Summary of immunoprecipitation (IP) and E1’ or p62-E1 ELISA resultsa.
Ryan J. Malonis (7832156) +20 more
core +1 more source

