Results 1 to 10 of about 227,914 (337)
Macronutrient intake modulates impact of EcoRI polymorphism of ApoB gene on lipid profile and inflammatory markers in patients with type 2 diabetes [PDF]
Scientific Reports, 2022 We sought to examine whether dietary intakes may affect the relationship between ApoB EcoRI and lipid profile, as well as serum inflammatory markers, in patients with type 2 diabetes (T2DM). This current study consisted of 648 diabetic patients.
Faezeh Abaj, Fariba Koohdani
doaj +2 more sources
Insight into Structure and Function of EcoRI Through Detailed Characterization of Nitroxide Side Chains Using Molecular Dynamics Simulations [PDF]
, 2011 This thesis describes molecular dynamics simulations used in an effort to investigate the orientation and dynamics of the methane thiosulfonate spin label (MTSSL) on solvent exposed sites of the loop (R131C) and beta strand (S180C) of the specific EcoRI ...
Rajapakse, Gayathri Ayasna
core +1 more source
Sequences spanning the EcoRI substrate site [PDF]
Nucleic Acids Research, 1975 Substrate recognition by the EcoRI restriction endonuclease was investigated by analysis of the nucleotide sequences at the sites of enzymatic cleavage in various DNA molecules. 5'-end labeling and homochromatographic fingerprinting led to the determination of a 17-base-pair sequence spanning the EcoRI site of simian virus 40 DNA and a 15-base-pair ...
David E. Garfin +2 more
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The Kinetic Mechanism of EcoRI Endonuclease [PDF]
Journal of Biological Chemistry, 1999 Steady-state parameters governing cleavage of pBR322 DNA by EcoRI endonuclease are highly sensitive to ionic environment, with K(m) and k(cat) increasing 1,000-fold and 15-fold, respectively, when ionic strength is increased from 0.059 to 0.23 M. By contrast, pre-steady-state analysis has shown that recognition, as well as first and second strand ...
David J. Wright +2 more
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Determinants of EcoRI endonuclease sequence discrimination. [PDF]
Proceedings of the National Academy of Sciences, 1989 The arginine at position 200 of EcoRI endonuclease is thought to make two hydrogen bonds to the guanine of the sequence GAATTC and thus be an important determinant of sequence discrimination. Arg-200 was replaced by each of the other 19 naturally occurring amino acids, and the mutant endonucleases were assessed for activities in vivo and in vitro.
Michael C. Needels +5 more
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Hydrogel bead-based isothermal detection (BEAD-ID) for assessing the activity of DNA-modifying enzymes [PDF]
iScienceSummary: DNA-modifying enzymes are crucial in biological processes and have significant clinical implications. Traditional quantification methods often overlook enzymatic activity, the true determinants of enzymes’ functions.
Kathrine Nygaard Borg +9 more
doaj +2 more sources
PLoS ONE, 2016 8-oxoguanine is one of the most abundant and impactful oxidative DNA lesions. However, the reasons underlying its effects, especially those not directly explained by the altered base pairing ability, are poorly understood.
Anton V Endutkin +2 more
exaly +2 more sources
Diffusion of the Restriction Nuclease EcoRI along DNA [PDF]
Journal of Molecular Biology, 2010 Many specific sequence DNA binding proteins locate their target sequence by first binding to DNA nonspecifically, then by linearly diffusing or hopping along DNA until either the protein dissociates from the DNA or it finds the recognition sequence. We have devised a method for measuring one-dimensional diffusion along DNA based on the ratio of the ...
Donald C, Rau, Nina Y, Sidorova
exaly +3 more sources
Analisis 6 DNA Rekombinan dengan enzim EcoRI
Journal of Biological Researches, 1997 Amylase enzyme from Endomycopsis fibuligera capable to hidrolize starch into glucose. Insertion of amylase gene from Endomycopsis fibuligera into yeast (Saccharomyces cereviceae) will be able to increase the function of yeast (S.cereviceae) to digest ...
Ni Nyoman Tri Puspaningsih +2 more
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Peptide Inhibitor Assay for Allocating Functionally Important Accessible Sites Throughout a Protein Chain: Restriction Endonuclease EcoRI as a Model Protein System [PDF]
BioTechFunctionally important amino acid sequences in proteins are often located at multiple sites. Three-dimensional structural analysis and site-directed mutagenesis may be performed to allocate functional sites for understanding structure‒function ...
Joji M. Otaki
doaj +2 more sources