Results 1 to 10 of about 223,024 (272)

An EcoRI polymorphism for the PLAUR gene [PDF]

Nucleic Acids Research, 1991
Udgivelsesdato: 1991-Dec ...
A. D. Børglum, A. Byskov, CUBELLIS, MARIA VITTORIA, T. A. Kruse   +3 more
exaly   +5 more sources

Analisis 6 DNA Rekombinan dengan enzim EcoRI

Journal of Biological Researches, 1997
Amylase enzyme from Endomycopsis fibuligera capable to hidrolize starch into glucose. Insertion of amylase gene from Endomycopsis fibuligera into yeast (Saccharomyces cereviceae) will be able to increase the function of yeast (S.cereviceae) to digest ...
Ni Nyoman Tri Puspaningsih, Akhmaloka Akhmaloka, Afaf Baktir   +2 more
doaj   +2 more sources

Peptide Inhibitor Assay for Allocating Functionally Important Accessible Sites Throughout a Protein Chain: Restriction Endonuclease EcoRI as a Model Protein System [PDF]

BioTech
Functionally important amino acid sequences in proteins are often located at multiple sites. Three-dimensional structural analysis and site-directed mutagenesis may be performed to allocate functional sites for understanding structure‒function ...
Joji M. Otaki
doaj   +2 more sources

Tomato protoplast DNA transformation: physical linkage and recombination of exogenous DNA sequences [PDF]

, 1987
Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication.
A Crossway, A Deshayes, B Reiss, BR Thomas, C-L Hsiao, CP Meredith, DT Stinchcomb, EA Shahin, FA Krens, G Scangos, HC Birnboim, HE Ruley, I Potrykus, J Messing, J Paszkowski, Jacques Hille, JD Rochaix, K Struhl, K Wernars, KC Reed, KR Folger, M Koornneef, M Perucho, Maarten Jongsma, Maarten Koornneef, MG Murray, MW Bevan, O Smithies, P Zabel, Pim Zabel, PW Rigby, R Hain, R Peerbolte, RA Anderson, RD Shillito, S Kato, SN Cohen, T Maniatis, TL Adams   +38 more
core   +16 more sources

Heparin inhibits EcoRI endonuclease cleavage of DNA at certain EcoRI sites [PDF]

Nucleic Acids Research, 1990
Studies presented here demonstrate that heparin inhibits EcoRI endonuclease cleavage of DNA whereas related proteoglycans show no effect. The inhibition occurs at particular EcoRI sites that are near or overlap with palindromic sequences in the murine lambda 5 and Lyt-2 genes.
J Z, Chen, L A, Herzenberg, L A, Herzenberg   +2 more
openaire   +2 more sources

The Kinetic Mechanism of EcoRI Endonuclease [PDF]

Journal of Biological Chemistry, 1999
Steady-state parameters governing cleavage of pBR322 DNA by EcoRI endonuclease are highly sensitive to ionic environment, with K(m) and k(cat) increasing 1,000-fold and 15-fold, respectively, when ionic strength is increased from 0.059 to 0.23 M. By contrast, pre-steady-state analysis has shown that recognition, as well as first and second strand ...
D J, Wright, W E, Jack, P, Modrich
openaire   +2 more sources

Effects of 2'-O-methyl nucleotide substitution on EcoRI endonuclease cleavage activities. [PDF]

PLoS ONE, 2013
To investigate the effect of sugar pucker conformation on DNA-protein interactions, we used 2'-O-methyl nucleotide (2'-OMeN) to modify the EcoRI recognition sequence -TGAATTCT-, and monitored the enzymatic cleavage process using FRET method.
Guojie Zhao, Bin Zhao, Zhaoxue Tong, Runqing Mu, Yifu Guan   +4 more
doaj   +1 more source

SMC is recruited to oriC by ParB and promotes chromosome segregation in Streptococcus pneumoniae [PDF]

, 2011
Segregation of replicated chromosomes is an essential process in all organisms. How bacteria, such as the oval-shaped human pathogen Streptococcus pneumoniae, efficiently segregate their chromosomes is poorly understood.
Avery, Baerends, Bloom, Bouthier de la Tour, Britton, Dagkessamanskaia, Danilova, Eberhardt, Errington, Fadda, Ferrandiz, Fogel, Gasc, Gerdes, Glaser, Graumann, Gruber, Guthlein, Halfmann, Hendriksen, Hoskins, Hudson, Hui, Ireton, Jun, Jun, Katayama, Kloosterman, Kornberg, Lanie, Leenhouts, Lin, Lindow, Livny, Lynch, Martin, Mascarenhas, Mitchell, Moriya, Mott, Murray, Nasmyth, O'Brien, Onn, Ptacin, Reyes-Lamothe, Ringgaard, Salje, Sambrook, Schofield, Scholefield, Sebert, Soppa, Sullivan, Terzaghi, Thanbichler, Toro, van der Poll, Weiser, Yu, Zakrzewska-Czerwinska, Zhao   +61 more
core   +5 more sources

The X-X-/E+E+ genotype of the XbaI/EcoRI polymorphisms of the apolipoprotein B gene as a marker of coronary artery disease in a Brazilian sample

Brazilian Journal of Medical and Biological Research, 2003
Studies that consider polymorphisms within the apolipoprotein B (apo B) gene as risk factors for coronary artery disease (CAD) have reported conflicting results.
M. Scartezini, M.A. Zago, E.A. Chautard-Freire-Maia, A. Pazin-Filho, J.A. Marin-Neto, J.K.S. Hotta, A.J. Nascimento, J.E. Dos-Santos   +7 more
doaj   +1 more source

A new and versatile method for the successful conversion of AFLP-TM markers into simple single locus markers [PDF]

, 2003
Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required.
Brugmans, B.W., Eck, H., van, Hulst, R.G.M., van der, Lindhout, W.H., Visser, R.G.F.   +4 more
core   +2 more sources