Results 11 to 20 of about 227,914 (337)

A Simple and Adaptable Method for Cloning Genes Into Transposon Vectors Using Topo and Restriction Systems for Chicken Embryo Transgenesis [PDF]

open access: yesBio-Protocol
Transposon-based genetic transformation enables stable transgene integration in avian genomes and is increasingly used in the development of transgenic chickens for enhanced disease resistance, productivity, and biopharmaceutical applications ...
Pamela Kirimi   +5 more
doaj   +2 more sources

Molecular variation of Trypanosoma brucei subspecies as revealed by AFLP fingerprinting [PDF]

open access: yes, 2002
Genetic analysis of Trypanosoma spp. depends on the detection of variation between strains. We have used the amplified fragment length polymorphism (AFLP) technique to develop a convenient and reliable method for genetic characterization of Trypanosome ...
Agbo, E.E.C.   +3 more
core   +17 more sources

Heparin inhibits EcoRI endonuclease cleavage of DNA at certain EcoRI sites [PDF]

open access: yesNucleic Acids Research, 1990
Studies presented here demonstrate that heparin inhibits EcoRI endonuclease cleavage of DNA whereas related proteoglycans show no effect. The inhibition occurs at particular EcoRI sites that are near or overlap with palindromic sequences in the murine lambda 5 and Lyt-2 genes.
J Z, Chen   +2 more
openaire   +2 more sources

Effects of 2'-O-methyl nucleotide substitution on EcoRI endonuclease cleavage activities. [PDF]

open access: yesPLoS ONE, 2013
To investigate the effect of sugar pucker conformation on DNA-protein interactions, we used 2'-O-methyl nucleotide (2'-OMeN) to modify the EcoRI recognition sequence -TGAATTCT-, and monitored the enzymatic cleavage process using FRET method.
Guojie Zhao   +4 more
doaj   +1 more source

SMC is recruited to oriC by ParB and promotes chromosome segregation in Streptococcus pneumoniae [PDF]

open access: yes, 2011
Segregation of replicated chromosomes is an essential process in all organisms. How bacteria, such as the oval-shaped human pathogen Streptococcus pneumoniae, efficiently segregate their chromosomes is poorly understood.
Avery   +61 more
core   +5 more sources

The X-X-/E+E+ genotype of the XbaI/EcoRI polymorphisms of the apolipoprotein B gene as a marker of coronary artery disease in a Brazilian sample

open access: yesBrazilian Journal of Medical and Biological Research, 2003
Studies that consider polymorphisms within the apolipoprotein B (apo B) gene as risk factors for coronary artery disease (CAD) have reported conflicting results.
M. Scartezini   +7 more
doaj   +1 more source

Identificação de híbridos de citros resistentes à mancha-marrom-de-alternária por meio de fAFLP e testes de patogenicidade Alternaria brown spot resistant citrus hybrid identification by means of fAFLP and pathogenicity tests

open access: yesPesquisa Agropecuária Brasileira, 2007
O objetivo deste trabalho foi identificar híbridos, oriundos de hibridações controladas entre 'Folha Murcha' x 'Ponkan' e testá-los quanto à resistência a Alternaria alternata f. sp. citri.
Edvan Alves Chagas   +9 more
doaj   +1 more source

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