Results 11 to 20 of about 228,317 (322)

Conformational Transitions as Determinants of Specificity for the DNA Methyltransferase EcoRI

Journal of Biological Chemistry, 2006
Norbert O Reich
exaly   +2 more sources

Heparin inhibits EcoRI endonuclease cleavage of DNA at certain EcoRI sites [PDF]

Nucleic Acids Research, 1990
Studies presented here demonstrate that heparin inhibits EcoRI endonuclease cleavage of DNA whereas related proteoglycans show no effect. The inhibition occurs at particular EcoRI sites that are near or overlap with palindromic sequences in the murine lambda 5 and Lyt-2 genes.
J Z, Chen, L A, Herzenberg, L A, Herzenberg   +2 more
openaire   +2 more sources

An EcoRI RFLP at the D10S103 locus

Nucleic Acids Research, 1990
Diane Miller, D. Smailus, Paul J. Goodfellow   +2 more
openalex   +5 more sources

The Kinetic Mechanism of EcoRI Endonuclease [PDF]

Journal of Biological Chemistry, 1999
Steady-state parameters governing cleavage of pBR322 DNA by EcoRI endonuclease are highly sensitive to ionic environment, with K(m) and k(cat) increasing 1,000-fold and 15-fold, respectively, when ionic strength is increased from 0.059 to 0.23 M. By contrast, pre-steady-state analysis has shown that recognition, as well as first and second strand ...
D J, Wright, W E, Jack, P, Modrich
openaire   +2 more sources

Effects of 2'-O-methyl nucleotide substitution on EcoRI endonuclease cleavage activities. [PDF]

PLoS ONE, 2013
To investigate the effect of sugar pucker conformation on DNA-protein interactions, we used 2'-O-methyl nucleotide (2'-OMeN) to modify the EcoRI recognition sequence -TGAATTCT-, and monitored the enzymatic cleavage process using FRET method.
Guojie Zhao, Bin Zhao, Zhaoxue Tong, Runqing Mu, Yifu Guan   +4 more
doaj   +1 more source

Molecular variation of Trypanosoma brucei subspecies as revealed by AFLP fingerprinting [PDF]

, 2002
Genetic analysis of Trypanosoma spp. depends on the detection of variation between strains. We have used the amplified fragment length polymorphism (AFLP) technique to develop a convenient and reliable method for genetic characterization of Trypanosome ...
Agbo, E.E.C., Claassen, H.J.H.M., Majiwa, P.A.O., Pas, M.F.W., te   +3 more
core   +9 more sources

A new and versatile method for the successful conversion of AFLP-TM markers into simple single locus markers [PDF]

, 2003
Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required.
Brugmans, B.W., Eck, H., van, Hulst, R.G.M., van der, Lindhout, W.H., Visser, R.G.F.   +4 more
core   +2 more sources

The X-X-/E+E+ genotype of the XbaI/EcoRI polymorphisms of the apolipoprotein B gene as a marker of coronary artery disease in a Brazilian sample

Brazilian Journal of Medical and Biological Research, 2003
Studies that consider polymorphisms within the apolipoprotein B (apo B) gene as risk factors for coronary artery disease (CAD) have reported conflicting results.
M. Scartezini, M.A. Zago, E.A. Chautard-Freire-Maia, A. Pazin-Filho, J.A. Marin-Neto, J.K.S. Hotta, A.J. Nascimento, J.E. Dos-Santos   +7 more
doaj   +1 more source

SMC is recruited to oriC by ParB and promotes chromosome segregation in Streptococcus pneumoniae [PDF]

, 2011
Segregation of replicated chromosomes is an essential process in all organisms. How bacteria, such as the oval-shaped human pathogen Streptococcus pneumoniae, efficiently segregate their chromosomes is poorly understood.
Avery, Baerends, Bloom, Bouthier de la Tour, Britton, Dagkessamanskaia, Danilova, Eberhardt, Errington, Fadda, Ferrandiz, Fogel, Gasc, Gerdes, Glaser, Graumann, Gruber, Guthlein, Halfmann, Hendriksen, Hoskins, Hudson, Hui, Ireton, Jun, Jun, Katayama, Kloosterman, Kornberg, Lanie, Leenhouts, Lin, Lindow, Livny, Lynch, Martin, Mascarenhas, Mitchell, Moriya, Mott, Murray, Nasmyth, O'Brien, Onn, Ptacin, Reyes-Lamothe, Ringgaard, Salje, Sambrook, Schofield, Scholefield, Sebert, Soppa, Sullivan, Terzaghi, Thanbichler, Toro, van der Poll, Weiser, Yu, Zakrzewska-Czerwinska, Zhao   +61 more
core   +5 more sources

Identificação de híbridos de citros resistentes à mancha-marrom-de-alternária por meio de fAFLP e testes de patogenicidade Alternaria brown spot resistant citrus hybrid identification by means of fAFLP and pathogenicity tests

Pesquisa Agropecuária Brasileira, 2007
O objetivo deste trabalho foi identificar híbridos, oriundos de hibridações controladas entre 'Folha Murcha' x 'Ponkan' e testá-los quanto à resistência a Alternaria alternata f. sp. citri.
Edvan Alves Chagas, Jairo Osvaldo Cazetta, Eliana Gertrudes Macedo Lemos, Moacir Pasqual, Antonio de Goes, Jose Darlan Ramos, Rafael Pio, Wilson Barbosa, Vander Mendonça, Luis Alberto Ambrosio   +9 more
doaj   +1 more source