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A rapid procedure for purification of EcoRI endonuclease
Biochemical and Biophysical Research Communications, 1977Abstract A convenient and rapid procedure has been developed to purify restriction endonuclease Eco RI. The method involves sonication of cells at low ionic strength, precipitation of the endonuclease with Polymin P (a polyethyleneimine), elution of the enzyme from the Polymin P precipitate, ammonium sulfate precipitation and chromatography on ...
J, Sümegi +3 more
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RFLP Standardization Report for C4/EcoRI
1989The panel of core cell lines cut with restriction enzyme MspI and hybridized with the C4 probe revealed a very simple pattern (Fig. 1). Two fragments were identified, of which only the first was polymorphic (1.37 kb) (Table 1).
M. Segall +3 more
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RFLP Standardization Report for 210H/EcoRI
1989The 210H/MspI system identified six standard fragments in the core cell lines as shown in Table 1 and Figure 1. Much of the data received for this system was difficult to interpret because all the fragments were of low molecular weight and thus produced indistinct bands.
M. Segall +3 more
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Bending and flexibility of methylated and unmethylated EcoRI DNA
Journal of Molecular Biology, 2002We used cyclization kinetics experiments and Monte Carlo simulations to determine a structural model for a DNA decamer containing the EcoRI restriction site. Our findings agree well with recent crystal and NMR structures of the EcoRI dodecamer, where an overall bend of seven degrees is distributed symmetrically over the molecule.
Dafna, Nathan, Donald M, Crothers
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RFLP Standardization Report for C2/EcoRI
1989C2/EcoRI blots were received from two laboratories. The C2/EcoRI system identified two fragments on the core cell lines (Table 1 and Fig. 1). The two fragments were complementary except for one discordance (cell 9060), which had a different fragment in each of the two blots. No other resolution of discrepancies was required.
M. Segall +3 more
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2019
The nucleotide sequence of the rat liver tyrosine aminotransferase gene EcoRI-fragment 3677 b. p. in length containig exones F, G, H, I, J, K (in part) and intrones 5, 6, 7, 8, 9, 10 was determined.
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The nucleotide sequence of the rat liver tyrosine aminotransferase gene EcoRI-fragment 3677 b. p. in length containig exones F, G, H, I, J, K (in part) and intrones 5, 6, 7, 8, 9, 10 was determined.
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Veränderung der Sequenzspezifität der Restriktionsendonuklease EcoRI
2004[no abstract]
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How the EcoRI endonuclease recognizes and cleaves DNA
BioEssays, 1992AbstractOne popular recombinant DNA tool is the EcoRI endonuclease, which cleaves DNA at GAATTC sites and serves as a paradigm for sequence specific DNA‐enzyme interactions. The recently revised X‐ray crystal structure of an EcoRI‐DNA complex reveals EcoRI employs novel DNA recognition motifs, a four α‐helix bundle and two extended chains, which ...
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