Results 241 to 250 of about 165,447 (318)

The Tetracycline‐Inducible/CRISPR‐Cas9 System is an Efficient Tool for Studying Gene Function in Phytophthora sojae

open access: yesMolecular Plant Pathology, Volume 26, Issue 6, June 2025.
The present study presents a novel approach combining a tetracycline‐inducible system (Tet‐On) and CRISPR‐Cas9 techniques to investigate the function of essential genes in Phytophthora sojae. ABSTRACT The present study presents a novel approach combining a tetracycline‐inducible system (Tet‐On) and CRISPR‐Cas9 techniques to investigate the function of ...
Chengcheng Li   +5 more
wiley   +1 more source

Guard cell‐specific glycine decarboxylase manipulation affects Arabidopsis photosynthesis, growth and stomatal behavior

open access: yesNew Phytologist, Volume 246, Issue 5, Page 2102-2117, June 2025.
Summary Photorespiration is a mandatory metabolic repair shunt of carbon fixation by the Calvin–Benson cycle in oxygenic phototrophs. Its extent depends mainly on the CO2 : O2 ratio in chloroplasts, which is regulated via stomatal movements. Despite a comprehensive understanding of the role of photorespiration in mesophyll cells, its role in guard ...
Hu Sun   +4 more
wiley   +1 more source

TaWUS‐like‐5D affects grain weight and filling by inhibiting the expression of sucrose and trehalose metabolism‐related genes in wheat grain endosperm

open access: yesPlant Biotechnology Journal, Volume 23, Issue 6, Page 2018-2033, June 2025.
Summary Plant‐specific WUSCHEL‐related homeobox (Wox) transcription factors (TFs) are crucial for plant growth and development. However, the molecular mechanism of Wox‐mediated regulation of thousand kernel weight (TKW) in crops remains elusive. In this research, we identified a major TKW‐associated quantitative trait locus (QTL) on wheat chromosome ...
Hongxia Liu   +10 more
wiley   +1 more source

Cas9‐Rep fusion tethers donor DNA in vivo and boosts the efficiency of HDR‐mediated genome editing

open access: yesPlant Biotechnology Journal, Volume 23, Issue 6, Page 2006-2017, June 2025.
Summary Genome editing based on the homology‐directed repair (HDR) pathway enables scar‐free and precise genetic manipulations. However, the low frequency of HDR hinders its application in plant genome editing. In this study, we engineered the fusion of Cas9 and a viral replication protein (Rep) as a molecular bridge to tether donor DNA in vivo, which ...
Zhentao Zhou   +7 more
wiley   +1 more source

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