Results 241 to 250 of about 165,447 (318)
A sequencing-based screening method identifies regulators of EGFR signaling from nonviable mutants in <i>Caenorhabditis elegans</i>. [PDF]
Schwartz HT, Sternberg PW.
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The present study presents a novel approach combining a tetracycline‐inducible system (Tet‐On) and CRISPR‐Cas9 techniques to investigate the function of essential genes in Phytophthora sojae. ABSTRACT The present study presents a novel approach combining a tetracycline‐inducible system (Tet‐On) and CRISPR‐Cas9 techniques to investigate the function of ...
Chengcheng Li+5 more
wiley +1 more source
Silent but significant: Functional elucidation of a synonymous ATP7B mutation in Wilson's disease pedigrees. [PDF]
Zhang Q, Xie X, Li H, Li Y, Huang H.
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Summary Photorespiration is a mandatory metabolic repair shunt of carbon fixation by the Calvin–Benson cycle in oxygenic phototrophs. Its extent depends mainly on the CO2 : O2 ratio in chloroplasts, which is regulated via stomatal movements. Despite a comprehensive understanding of the role of photorespiration in mesophyll cells, its role in guard ...
Hu Sun+4 more
wiley +1 more source
Nuclear Accumulation of Bm65 Aggregate Is Blocked by Mutations in the Nuclear Export Sequence of Bm65. [PDF]
Li G+9 more
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Summary Plant‐specific WUSCHEL‐related homeobox (Wox) transcription factors (TFs) are crucial for plant growth and development. However, the molecular mechanism of Wox‐mediated regulation of thousand kernel weight (TKW) in crops remains elusive. In this research, we identified a major TKW‐associated quantitative trait locus (QTL) on wheat chromosome ...
Hongxia Liu+10 more
wiley +1 more source
Morphology-engineered alleviation of mycelial aggregation in <i>Streptomyces</i> chassis for potentiated production of secondary metabolites. [PDF]
Liu S+5 more
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Cas9‐Rep fusion tethers donor DNA in vivo and boosts the efficiency of HDR‐mediated genome editing
Summary Genome editing based on the homology‐directed repair (HDR) pathway enables scar‐free and precise genetic manipulations. However, the low frequency of HDR hinders its application in plant genome editing. In this study, we engineered the fusion of Cas9 and a viral replication protein (Rep) as a molecular bridge to tether donor DNA in vivo, which ...
Zhentao Zhou+7 more
wiley +1 more source