Results 271 to 280 of about 228,317 (322)

Jub-induced phosphorylation of Warts inhibits its activity and recruitment into biomolecular condensates. [PDF]

Cell Rep
Kirichenko E, Rauskolb C, Mansuria K, Michell DF, Niloy MS, Irvine KD.   +5 more
europepmc   +1 more source

<i>Enterococcus faecalis</i> requires unsaturated fatty acids to overcome toxicity of environmental saturated fatty acids. [PDF]

Microbiology (Reading)
Zou Q, Dong H, Cronan JE.
europepmc   +1 more source

Mutations in components of the TREX-2 complex result in misexpression of the Kelch-domain F-Box protein KFB39 promoter in Arabidopsis thaliana

Matthes MC, Kurup S, Pickett JA, Napier JA.   +3 more
europepmc   +1 more source

The E3 ubiquitin ligase SPRYD3-MYCBP2(PAM) regulates mitotic cell fate and ubiquitination of USP11 to control spindle assembly. [PDF]

J Biol Chem
Turi da Fonte Dias AR, Hoffmann I.
europepmc   +1 more source

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Bending and flexibility of methylated and unmethylated EcoRI DNA

Journal of Molecular Biology, 2002
We used cyclization kinetics experiments and Monte Carlo simulations to determine a structural model for a DNA decamer containing the EcoRI restriction site. Our findings agree well with recent crystal and NMR structures of the EcoRI dodecamer, where an overall bend of seven degrees is distributed symmetrically over the molecule.
Dafna, Nathan, Donald M, Crothers
exaly   +3 more sources

EcoRI DNA methyltransferase-DNA interactions

Biochemistry, 1992
We present a novel strategy with synthetic hemimethylated DNA substrates containing uracil for thymine and inosine for guanosine replacements and EcoRI DNA methyltransferase to characterize the importance of major groove hydrophobic groups to the sequence-specific modification of DNA.
N O, Reich, M J, Danzitz
openaire   +2 more sources

Spectroscopic studies on the interaction between EcoRI and CdS QDs and conformation of EcoRI in EcoRI-CdS QDs bioconjugates

Physical Chemistry Chemical Physics, 2012
Water-soluble fluorescent quantum dots (QDs) have been widely used in biological and biomedical fields, and the interaction between QDs and proteins and the conformational structure of the protein in the bioconjugate has attracted increasing attention.
Yonghai, Song, Dan, Luo, Shuhong, Ye, Mei, Huang, Dandan, Zhong, Zhenzhong, Huang, Haoqing, Hou, Li, Wang   +7 more
openaire   +2 more sources

Oriented immobilization of restriction endonuclease ecori

Journal of Molecular Recognition, 1996
Two activated matrices have been developed to determine whether immobilization chemistry can be used to orient proteins on a support. Restriction endonuclease EcoRI from Escherichia coli RY13 (E.C.3.1.23.13) was used as a model in these studies. Thiol-activated Sephadex G-10 was used to couple the EcoRI endonuclease through its free sulfhydryl, while ...
M, Bircakova, M, Truksa, W H, Scouten
openaire   +2 more sources

EcoRI-sensitive mutation of T7 phage

Molecular and General Genetics MGG, 1977
It is known that DNA from phage T7 is not cleaved by restriction endonuclease EcoRI. However, DNA from phage T7am28 (gene 5) mutant (Studier, 1969) was found to be cleaved by the endonuclease at one site. The site is located at 0.46 fractional length from the left end of the molecule.
Y, Tsujimoto, H, Ogawa
openaire   +2 more sources

Temperature-sensitive mutants of the EcoRI endonuclease

Journal of Molecular Biology, 1997
The EcoRI endonuclease is an important recombinant DNA tool and a paradigm of sequence-specific DNA-protein interactions. We have isolated temperature-sensitive (TS) EcoRI endonuclease mutants (R56Q, G78D, P90S, V97I, R105K, M157I, C218Y, A235E, M255I, T261I and L263F) and characterized activity in vivo and in vitro.
R S, Muir, H, Flores, N D, Zinder, P, Model, X, Soberon, J, Heitman   +5 more
openaire   +2 more sources