Results 281 to 290 of about 228,317 (322)

RFLP Standardization Report for DQ Beta/EcoRI

1989
The DQ beta/EcoRI system identified 13 fragments in the core cell lines (Fig. 1). Locus assignments in this system (Table 1) showed fragments 1,2,4,5,6,10, & 13 to be DQB specific, the remaining fragments cross-hybridizing with both DRB and DPB (Table 3 in the DR Beta/EcoRI report).
S. Tonks, V. Groves, H. Betuel, J. L. Bidwell, M. L. Garavoy, R. Lechler, S. Naito, J. Bodmer, J. Trowsdale   +8 more
openaire   +1 more source

An electrochemiluminescence biosensor for endonuclease EcoRI detection

Biosensors and Bioelectronics, 2017
Endonucleases cleavage of DNA plays an important role in biological and medicinal chemistry. This work was going to develop a reliable and sensitive electrochemiluminescent (ECL) biosensor for detecting endonucleases by using gold nanoparticles graphene composite (GNPs-graphene) as a signal amplifier.
Yingjie, Li, Yuqin, Li, Yaoyu, Wu, Fushen, Lu, Yaowen, Chen, Wenhua, Gao   +5 more
openaire   +2 more sources

Kinetic mechanism of the EcoRI DNA methyltransferase

Biochemistry, 1991
We present a kinetic analysis of the EcoRI DNA N6-adenosine methyltransferase (Mtase). The enzyme catalyzes the S-adenosylmethionine (AdoMet)-dependent methylation of a short, synthetic 14 base pair DNA substrate and plasmid pBR322 DNA substrate with kcat/Km values of 0.51 X 10(8) and 4.1 X 10(8) s-1 M-1, respectively. The Mtase is thus one of the most
N O, Reich, N, Mashhoon
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EcoRI variant N199H has enhanced specific activity

Gene, 1996
The Asn199 residue of the EcoRI restriction endonuclease has been replaced with other amino acids to investigate whether it mediates nucleotide recognition or catalytic activity. Cassette mutagenesis gave variants of EcoRI: N199D, N199H, N199L, N199R, N199S and N199V. Their relative cleavage rates were found to be in the following order: N199H > EcoRI (
Kim, JJ, Min, Kyung-Tai, Kim, MH, Augh, SJ, Kim, BD, Lee, DS   +5 more
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Effect of high of pressure of EcoRI reactions

Journal of Molecular Recognition, 1994
AbstractThe effect of pressure on reactions of restriction endonucleases was investigated. No obvious irreversible (after) effect was observed for EcoRI, while a considerable irreversible inactivation was found for BamHI. Thus the EcoRI reactions against λ DNA, pBR322 and pBluescript were studied under high pressure and little effect was observed on ...
H, Kabata, A, Nomura, N, Shimamoto, S, Kunugi   +3 more
openaire   +2 more sources

Footprinting of EcoRI endonuclease at high pressure

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1992
Hydroxyl radicals generated by irradiation with gamma rays have been used to footprint EcoRI endonuclease with single base pair resolution at pressures up to 144 MPa. At atmospheric pressure (0.1 MPa) a 10 base pair footprint was found. With increasing pressure three types of responses were observed: (1) bases distant from the recognition sequence ...
openaire   +2 more sources

A new EcoRI polymorphism at the D21S13 locus

Human Genetics, 1990
The D21S13 locus has shown linkage to a gene for familial Alzheimer disease (FAD) on chromosome 21 (St. George-Hyslop et al. 1987). The limited informativeness of probes for this locus have hindered precise mapping of the FAD locus and analysis of nonallelic heterogeneity in FAD (Schellenberg et al. 1988; St. George-Hyslop et al. 1987).
S M, Pulst, J R, Korenberg, J, Greenwald, M, Carbone   +3 more
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Internal EcoRI-generated deletion in prophage Mu DNA

Biochemical and Biophysical Research Communications, 1980
Abstract The hybrid plasmid consisting of the plasmid pRP1.2 (derivative of RP4) genome and deleted prophage Mucts 62 genome which lost the central EcoRI fragment of DNA was constructed. The ability of deleted Mu phage to carry out E. coli chromosomal genes transposition was still retained.
S I, Alikhanian, A M, Kaplan, M A, Krupenko   +2 more
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Reversible inhibition of EcoRI with elevated pressure

Biochemical and Biophysical Research Communications, 1990
The endonuclease activity of EcoRI is completely inhibited at 200 MPa, 37 degrees C using the plasmid pBR 322 as a substrate. When assayed at 133 MPa approximately half the activity at atmospheric pressure was observed; from these data the standard molar volume change is estimated to be -80 cm3mol-1.
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EcoRI endonuclease cleavage map of bacteriophage P4-DNA

Virology, 1975
Abstract Satellite bacteriophage P4-DNA is cleaved by EcoRI endonuclease at three specific sites, 0.702, 0.737, and 0.985 fractional lengths as measured from the left end of the molecule. The EcoRI cleavage map was determined by electron microscopic length measurements and agarose-gel electrophoresis.
L, Goldstein, M, Thomas, R W, Davis
openaire   +2 more sources