Results 281 to 290 of about 225,565 (329)

Fine-tuning GBS data with comparison of reference and mock genome approaches for advancing genomic selection in less studied farmed species. [PDF]

BMC Genomics
Fischer D, Tapio M, Bitz O, Iso-Touru T, Kause A, Tapio I.   +5 more
europepmc   +1 more source

CRISPR-based dissection of miRNA binding sites using isogenic cell lines is hampered by pervasive noise. [PDF]

Nucleic Acids Res
Prajapat MK, Maria AG, Vidigal JA.
europepmc   +1 more source

The hypusine pathway in <i>Ixode</i>s <i>ricinus</i>: molecular cloning and validation of deoxyhypusine synthase as a novel target for drug discovery to treat and prevent vector borne diseases. [PDF]

Front Cell Infect Microbiol
Kaiser A, Agostinelli E, Tsikas D.
europepmc   +1 more source

Genome-wide mapping of cyclic AMP receptor protein binding in Enteroaggregative Escherichia coli reveals targeting of virulence-associated genes

Alhammadi MM, Hothersall J, Lloyd GS, Titman SV, Guest T, Browning DF, Grainger DC, Busby SJ, Haycocks JR.   +8 more
europepmc   +1 more source

The axonal ER couples translation and secretion machineries for local delivery of axonal transmembrane proteins to promote axonal development

Nguyen HH, Kersten N, Li CH, de Jong H, Arora T, Liolios T, Nguyen DTM, Bebelman MP, Altelaar M, Koppers M, Farías GG.   +10 more
europepmc   +1 more source

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Bending and flexibility of methylated and unmethylated EcoRI DNA

Journal of Molecular Biology, 2002
We used cyclization kinetics experiments and Monte Carlo simulations to determine a structural model for a DNA decamer containing the EcoRI restriction site. Our findings agree well with recent crystal and NMR structures of the EcoRI dodecamer, where an overall bend of seven degrees is distributed symmetrically over the molecule.
Donald M. Crothers, Dafna Nathan
exaly   +4 more sources

Spectroscopic studies on the interaction between EcoRI and CdS QDs and conformation of EcoRI in EcoRI-CdS QDs bioconjugates

Physical Chemistry Chemical Physics, 2012
Water-soluble fluorescent quantum dots (QDs) have been widely used in biological and biomedical fields, and the interaction between QDs and proteins and the conformational structure of the protein in the bioconjugate has attracted increasing attention.
Li Wang, Yonghai Song, Zhenzhong Huang, Dan Luo, Mei Huang, Dandan Zhong, Shuhong Ye, Haoqing Hou   +7 more
openaire   +3 more sources

Effect of high of pressure of EcoRI reactions

Journal of Molecular Recognition, 1994
AbstractThe effect of pressure on reactions of restriction endonucleases was investigated. No obvious irreversible (after) effect was observed for EcoRI, while a considerable irreversible inactivation was found for BamHI. Thus the EcoRI reactions against λ DNA, pBR322 and pBluescript were studied under high pressure and little effect was observed on ...
H. Kabata, Nobuo Shimamoto, Shigeru Kunugi, Akihiko Nomura   +3 more
openaire   +3 more sources

Temperature-sensitive mutants of the EcoRI endonuclease

Journal of Molecular Biology, 1997
The EcoRI endonuclease is an important recombinant DNA tool and a paradigm of sequence-specific DNA-protein interactions. We have isolated temperature-sensitive (TS) EcoRI endonuclease mutants (R56Q, G78D, P90S, V97I, R105K, M157I, C218Y, A235E, M255I, T261I and L263F) and characterized activity in vivo and in vitro.
R S Muir, Xavier Soberón, Norton D. Zinder, Peter Model, Joseph Heitman, Joseph Heitman, Humberto Flores   +6 more
openaire   +3 more sources