Results 281 to 290 of about 165,447 (318)

Label-free fluorescent assay for real-time monitoring site-specific DNA cleavage by EcoRI endonuclease.

In Analysis, 2012
DNA cleavage reaction catalyzed by nucleases is essential in many important biological processes and medicinal chemistry. Therefore, it is important to develop reliable and facile methods to assay nuclease activity.
Jing Deng, Yan Jin, Guozhen Chen, Lin Wang   +3 more
semanticscholar   +1 more source

Effect of high of pressure of EcoRI reactions

Journal of Molecular Recognition, 1994
AbstractThe effect of pressure on reactions of restriction endonucleases was investigated. No obvious irreversible (after) effect was observed for EcoRI, while a considerable irreversible inactivation was found for BamHI. Thus the EcoRI reactions against λ DNA, pBR322 and pBluescript were studied under high pressure and little effect was observed on ...
H. Kabata, Nobuo Shimamoto, Shigeru Kunugi, Akihiko Nomura   +3 more
openaire   +3 more sources

Temperature-sensitive mutants of the EcoRI endonuclease

Journal of Molecular Biology, 1997
The EcoRI endonuclease is an important recombinant DNA tool and a paradigm of sequence-specific DNA-protein interactions. We have isolated temperature-sensitive (TS) EcoRI endonuclease mutants (R56Q, G78D, P90S, V97I, R105K, M157I, C218Y, A235E, M255I, T261I and L263F) and characterized activity in vivo and in vitro.
R S Muir, Xavier Soberón, Norton D. Zinder, Peter Model, Joseph Heitman, Joseph Heitman, Humberto Flores   +6 more
openaire   +3 more sources

EcoRI DNA methyltransferase-DNA interactions

Biochemistry, 1992
We present a novel strategy with synthetic hemimethylated DNA substrates containing uracil for thymine and inosine for guanosine replacements and EcoRI DNA methyltransferase to characterize the importance of major groove hydrophobic groups to the sequence-specific modification of DNA.
Michael J. Danzitz, Norbert O. Reich
openaire   +3 more sources

A common EcoRI polymorphism at the ZFX locus

Human Molecular Genetics, 1992
The human pseudoautosomal region comprises a 2.6 megabase segment of the distal short arms of the X and Y chromosomes. Complete DNA sequence homology between the two sex chromosomes is found in this region, and is believed to be important in mediating X-Y pairing in male meiosis.
Larry Shapiro, Jay Ellison, Jay Ellison, Catherine Alles, Catherine Alles   +4 more
openaire   +3 more sources

Targeted base stacking disruption by the EcoRI DNA methyltransferase.

Biochemistry, 1996
We describe a novel fluorescence-based assay for detecting DNA conformational alterations within enzyme-DNA complexes. The target adenine for EcoRI DNA methyltransferase (GAATTC) was replaced with 2-aminopurine, which fluoresces upon excitation at 310 nm.
Barrett W. Allan, Norbert O. Reich
semanticscholar   +1 more source

A new EcoRI polymorphism at the D21S13 locus

Human Genetics, 1990
The D21S13 locus has shown linkage to a gene for familial Alzheimer disease (FAD) on chromosome 21 (St. George-Hyslop et al. 1987). The limited informativeness of probes for this locus have hindered precise mapping of the FAD locus and analysis of nonallelic heterogeneity in FAD (Schellenberg et al. 1988; St. George-Hyslop et al. 1987).
M Carbone, Stefan M. Pulst, Julie R. Korenberg, J. Greenwald   +3 more
openaire   +3 more sources

[12] Purification and properties of EcoRI endonuclease

1980
Publisher Summary This chapter discusses the purification and properties of EcoRI endonuclease. The Escherichia coli RI (EcoRI) DNA restriction and modification enzymes recognize a common twofold symmetrical hexanucleotide sequence in duplex DNA.
Paul Modrich, R A Rubin
openaire   +3 more sources

An electrochemiluminescence biosensor for endonuclease EcoRI detection

Biosensors and Bioelectronics, 2017
Endonucleases cleavage of DNA plays an important role in biological and medicinal chemistry. This work was going to develop a reliable and sensitive electrochemiluminescent (ECL) biosensor for detecting endonucleases by using gold nanoparticles graphene composite (GNPs-graphene) as a signal amplifier.
Yaowen Chen, Yaoyu Wu, Yuqin Li, Fushen Lu, Wenhua Gao, Yingjie Li   +5 more
openaire   +3 more sources

Structure and dynamics of a fluorescent DNA oligomer containing the EcoRI recognition sequence: fluorescence, molecular dynamics, and NMR studies.

Biochemistry, 1989
The self-complementary DNA decamer duplex d(CTGAATTCAG)2 and its modified counterpart d(CTGA[2AP]TTCAG)2, where the innermost adenine (6-aminopurine) has been replaced with the fluorescent analogue 2-aminopurine (2AP), have been studied by fluorescence ...
T. Nordlund, S. Andersson, Lennart Nilsson, Rudolf Rigler, A. Graslund, Larry W. McLaughlin   +5 more
semanticscholar   +1 more source