Results 21 to 30 of about 165,447 (318)
Restriction fragment length polymorphism (RFLP) analysis using a multilocus heat-inducible cytoplasmic heat-shock protein 70 (Hsp70) hybridization probe with EcoRI-digested genomic DNA was used in molecular typing of 129 Trichomonas vaginalis isolates ...
John C. Meade+6 more
semanticscholar +1 more source
The Investigation of Haplotype Phasing Efficiency at the PAH Gene Region in Iranian Family Trios [PDF]
"nBackground: The haplotype phasing is more useful than genotyping markers independently at carrier detection and prena­tal diagnosis of diseases. The PAH gene region contains several markers used in detection of PKU disease.
Z Fazeli, S Vallian
doaj +2 more sources
Optimization of the EcoRI*‐activity of EcoRI endonuclease [PDF]
sequences [5]. I have attempted to optimise the &OR*, activity through lowering of the ionic strength of the medium and by the addition of organic solvents. This study is of interest in elucidating the mechanism of interactjon between a nucleic acid sequence and a restriction endonuclease and furthermore for the evaluation of the useful range of ...
openaire +3 more sources
Characteristics of Glucose Oxidase Gene (GGOx) from Aspergillus niger IPBCC 08.610
Glucose oxidase is used in various industries for the development of enzymatic fuel cell. Based on prior studies, this compound is sourced from the local isolates of Aspergillus niger IPBCC 08.610, although investigations on the encoding gene have not ...
Popi Asri Kurniatin+5 more
doaj +1 more source
Overmethylation of DNAs by the EcoRI methylase
EcoRI methylase is able to catalyze methy incorporation into DNA at sequences other than the canonical EcoRI site. At high enzyme concentrations and over a wide range of pH and ionic strengths, EcoRI methylase modifies polyoma DNA (which contains one EcoRI site) at a number of sites.
Kathleen L. Berkner, William R. Folk
openaire +4 more sources
Distinct viral gene expression characterizes Epstein-Barr virus (EBV) infection in EBV-producing marmoset B-cell (B95-8) and EBV-associated gastric carcinoma (SNU719) cell lines.
Sun Hee Lee+11 more
doaj +1 more source
In this communication, we show that the EcoRI restriction endonuclease binds different classes of DNA sites in the same binding cleft. EcoRI generates widespread interest because it exhibits an extraordinary sequence selectivity to carry out its function
Katherine M. Stone+5 more
semanticscholar +1 more source
Formation of DNA−Protein Cross-Links Between γ-Hydroxypropanodeoxyguanosine and EcoRI
The toxicity of acrolein, an α,β-unsaturated aldehyde produced during lipid peroxidation, is attributable to its high reactivity toward DNA and cellular proteins.
Laurie Vanderveen+3 more
semanticscholar +1 more source
Bacillus thuringiensis (Bt) subsp. medellin (Btmed) produces parasporal crystalline inclusions which are toxic to mosquito larvae. It has been shown that the inclusions of this bacterium contain mainly proteins of 94, 68 and 28-30 kDa.
Nora Restrepo+5 more
doaj +1 more source
The chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro was isola ted from chloroplasts obtained by descontiuous sucrose gradient centrifugation. The restriction analysis with the enzymes HindIII, EcoRI and BamHI and their combination, allowed to ...
Sergio Echeverrigaray+2 more
doaj +1 more source