Results 21 to 30 of about 165,447 (318)

Genetic diversity of Trichomonas vaginalis clinical isolates determined by EcoRI restriction fragment length polymorphism of heat-shock protein 70 genes.

open access: yesAmerican Journal of Tropical Medicine and Hygiene, 2009
Restriction fragment length polymorphism (RFLP) analysis using a multilocus heat-inducible cytoplasmic heat-shock protein 70 (Hsp70) hybridization probe with EcoRI-digested genomic DNA was used in molecular typing of 129 Trichomonas vaginalis isolates ...
John C. Meade   +6 more
semanticscholar   +1 more source

The Investigation of Haplotype Phasing Efficiency at the PAH Gene Region in Iranian Family Trios [PDF]

open access: yesIranian Journal of Public Health, 2009
"nBackground: The haplotype phasing is more useful than genotyping markers independently at carrier detection and prena­tal diagnosis of diseases. The PAH gene region contains several markers used in detection of PKU disease.
Z Fazeli, S Vallian
doaj   +2 more sources

Optimization of the EcoRI*‐activity of EcoRI endonuclease [PDF]

open access: yesFEBS Letters, 1978
sequences [5]. I have attempted to optimise the &OR*, activity through lowering of the ionic strength of the medium and by the addition of organic solvents. This study is of interest in elucidating the mechanism of interactjon between a nucleic acid sequence and a restriction endonuclease and furthermore for the evaluation of the useful range of ...
openaire   +3 more sources

Characteristics of Glucose Oxidase Gene (GGOx) from Aspergillus niger IPBCC 08.610

open access: yesJurnal Kimia Valensi, 2020
Glucose oxidase is used in various industries for the development of enzymatic fuel cell. Based on prior studies, this compound is sourced from the local isolates of Aspergillus niger IPBCC 08.610, although investigations on the encoding gene have not ...
Popi Asri Kurniatin   +5 more
doaj   +1 more source

Overmethylation of DNAs by the EcoRI methylase

open access: yesNucleic Acids Research, 1978
EcoRI methylase is able to catalyze methy incorporation into DNA at sequences other than the canonical EcoRI site. At high enzyme concentrations and over a wide range of pH and ionic strengths, EcoRI methylase modifies polyoma DNA (which contains one EcoRI site) at a number of sites.
Kathleen L. Berkner, William R. Folk
openaire   +4 more sources

Characterization of a new CCCTC-binding factor binding site as a dual regulator of Epstein-Barr virus latent infection.

open access: yesPLoS Pathogens, 2023
Distinct viral gene expression characterizes Epstein-Barr virus (EBV) infection in EBV-producing marmoset B-cell (B95-8) and EBV-associated gastric carcinoma (SNU719) cell lines.
Sun Hee Lee   +11 more
doaj   +1 more source

Electron spin resonance shows common structural features for different classes of EcoRI-DNA complexes.

open access: yesAngewandte Chemie, 2008
In this communication, we show that the EcoRI restriction endonuclease binds different classes of DNA sites in the same binding cleft. EcoRI generates widespread interest because it exhibits an extraordinary sequence selectivity to carry out its function
Katherine M. Stone   +5 more
semanticscholar   +1 more source

Formation of DNA−Protein Cross-Links Between γ-Hydroxypropanodeoxyguanosine and EcoRI

open access: yesChemical Research in Toxicology, 2008
The toxicity of acrolein, an α,β-unsaturated aldehyde produced during lipid peroxidation, is attributable to its high reactivity toward DNA and cellular proteins.
Laurie Vanderveen   +3 more
semanticscholar   +1 more source

Cloning, Expression and Toxicity of a Mosquitocidal Toxin Gene of Bacillus thuringiensis subsp. medellin

open access: yesMemorias do Instituto Oswaldo Cruz, 1997
Bacillus thuringiensis (Bt) subsp. medellin (Btmed) produces parasporal crystalline inclusions which are toxic to mosquito larvae. It has been shown that the inclusions of this bacterium contain mainly proteins of 94, 68 and 28-30 kDa.
Nora Restrepo   +5 more
doaj   +1 more source

Restriction enzyme analysis of the chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro Análise de restrição do DNA cloroplástico de Phaseolus vulgaris vr. Rio Negro

open access: yesCiência Rural, 1996
The chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro was isola ted from chloroplasts obtained by descontiuous sucrose gradient centrifugation. The restriction analysis with the enzymes HindIII, EcoRI and BamHI and their combination, allowed to ...
Sergio Echeverrigaray   +2 more
doaj   +1 more source

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