Results 291 to 300 of about 225,565 (329)

EcoRI DNA methyltransferase-DNA interactions

Biochemistry, 1992
We present a novel strategy with synthetic hemimethylated DNA substrates containing uracil for thymine and inosine for guanosine replacements and EcoRI DNA methyltransferase to characterize the importance of major groove hydrophobic groups to the sequence-specific modification of DNA.
Michael J. Danzitz, Norbert O. Reich
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A common EcoRI polymorphism at the ZFX locus

Human Molecular Genetics, 1992
The human pseudoautosomal region comprises a 2.6 megabase segment of the distal short arms of the X and Y chromosomes. Complete DNA sequence homology between the two sex chromosomes is found in this region, and is believed to be important in mediating X-Y pairing in male meiosis.
Larry Shapiro, Jay Ellison, Jay Ellison, Catherine Alles, Catherine Alles   +4 more
openaire   +3 more sources

RFLP Standardization Report for DQ Beta/EcoRI

1989
The DQ beta/EcoRI system identified 13 fragments in the core cell lines (Fig. 1). Locus assignments in this system (Table 1) showed fragments 1,2,4,5,6,10, & 13 to be DQB specific, the remaining fragments cross-hybridizing with both DRB and DPB (Table 3 in the DR Beta/EcoRI report).
V. Groves, J. Bodmer, John Trowsdale, R. Lechler, M. L. Garavoy, S. Tonks, J. L. Bidwell, S. Naito, H. Betuel   +8 more
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An electrochemiluminescence biosensor for endonuclease EcoRI detection

Biosensors and Bioelectronics, 2017
Endonucleases cleavage of DNA plays an important role in biological and medicinal chemistry. This work was going to develop a reliable and sensitive electrochemiluminescent (ECL) biosensor for detecting endonucleases by using gold nanoparticles graphene composite (GNPs-graphene) as a signal amplifier.
Yaowen Chen, Yaoyu Wu, Yuqin Li, Fushen Lu, Wenhua Gao, Yingjie Li   +5 more
openaire   +3 more sources

[12] Purification and properties of EcoRI endonuclease

1980
Publisher Summary This chapter discusses the purification and properties of EcoRI endonuclease. The Escherichia coli RI (EcoRI) DNA restriction and modification enzymes recognize a common twofold symmetrical hexanucleotide sequence in duplex DNA.
Paul Modrich, R A Rubin
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Kinetic mechanism of the EcoRI DNA methyltransferase

Biochemistry, 1991
We present a kinetic analysis of the EcoRI DNA N6-adenosine methyltransferase (Mtase). The enzyme catalyzes the S-adenosylmethionine (AdoMet)-dependent methylation of a short, synthetic 14 base pair DNA substrate and plasmid pBR322 DNA substrate with kcat/Km values of 0.51 X 10(8) and 4.1 X 10(8) s-1 M-1, respectively. The Mtase is thus one of the most
Norbert O. Reich, Neda Mashhoon
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A new EcoRI polymorphism at the D21S13 locus

Human Genetics, 1990
The D21S13 locus has shown linkage to a gene for familial Alzheimer disease (FAD) on chromosome 21 (St. George-Hyslop et al. 1987). The limited informativeness of probes for this locus have hindered precise mapping of the FAD locus and analysis of nonallelic heterogeneity in FAD (Schellenberg et al. 1988; St. George-Hyslop et al. 1987).
M Carbone, Stefan M. Pulst, Julie R. Korenberg, J. Greenwald   +3 more
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Oriented immobilization of restriction endonuclease ecori

Journal of Molecular Recognition, 1996
Two activated matrices have been developed to determine whether immobilization chemistry can be used to orient proteins on a support. Restriction endonuclease EcoRI from Escherichia coli RY13 (E.C.3.1.23.13) was used as a model in these studies. Thiol-activated Sephadex G-10 was used to couple the EcoRI endonuclease through its free sulfhydryl, while ...
Martin Truksa, William H. Scouten, Miroslava Bircakova   +2 more
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A rapid procedure for purification of EcoRI endonuclease

Biochemical and Biophysical Research Communications, 1977
Abstract A convenient and rapid procedure has been developed to purify restriction endonuclease Eco RI. The method involves sonication of cells at low ionic strength, precipitation of the endonuclease with Polymin P (a polyethyleneimine), elution of the enzyme from the Polymin P precipitate, ammonium sulfate precipitation and chromatography on ...
János Sümegi, Pierre Chambon, Paul Hossenlopp, Danielle Breedveld   +3 more
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EcoRI-sensitive mutation of T7 phage

Molecular and General Genetics MGG, 1977
It is known that DNA from phage T7 is not cleaved by restriction endonuclease EcoRI. However, DNA from phage T7am28 (gene 5) mutant (Studier, 1969) was found to be cleaved by the endonuclease at one site. The site is located at 0.46 fractional length from the left end of the molecule.
Yoshihide Tsujimoto, Hideyuki Ogawa
openaire   +3 more sources