Results 291 to 300 of about 165,447 (318)

RFLP Standardization Report for DQ Beta/EcoRI

1989
The DQ beta/EcoRI system identified 13 fragments in the core cell lines (Fig. 1). Locus assignments in this system (Table 1) showed fragments 1,2,4,5,6,10, & 13 to be DQB specific, the remaining fragments cross-hybridizing with both DRB and DPB (Table 3 in the DR Beta/EcoRI report).
V. Groves, J. Bodmer, John Trowsdale, R. Lechler, M. L. Garavoy, S. Tonks, J. L. Bidwell, S. Naito, H. Betuel   +8 more
openaire   +5 more sources

Oriented immobilization of restriction endonuclease ecori

Journal of Molecular Recognition, 1996
Two activated matrices have been developed to determine whether immobilization chemistry can be used to orient proteins on a support. Restriction endonuclease EcoRI from Escherichia coli RY13 (E.C.3.1.23.13) was used as a model in these studies. Thiol-activated Sephadex G-10 was used to couple the EcoRI endonuclease through its free sulfhydryl, while ...
Martin Truksa, William H. Scouten, Miroslava Bircakova   +2 more
openaire   +2 more sources

A rapid procedure for purification of EcoRI endonuclease

Biochemical and Biophysical Research Communications, 1977
Abstract A convenient and rapid procedure has been developed to purify restriction endonuclease Eco RI. The method involves sonication of cells at low ionic strength, precipitation of the endonuclease with Polymin P (a polyethyleneimine), elution of the enzyme from the Polymin P precipitate, ammonium sulfate precipitation and chromatography on ...
János Sümegi, Pierre Chambon, Paul Hossenlopp, Danielle Breedveld   +3 more
openaire   +3 more sources

Divalent metal ions at the active sites of the EcoRV and EcoRI restriction endonucleases.

Biochemistry, 1995
Restriction enzymes cannot cleave DNA without a metal ion cofactor. The specificities of the EcoRV and EcoRI endonucleases for metals were studied by measuring DNA cleavage rates with several metal ions and with combinations of metal ions. Both EcoRV and
I. Vipond, Geoffrey S. Baldwin, S. Halford   +2 more
semanticscholar   +1 more source

EcoRI-sensitive mutation of T7 phage

Molecular and General Genetics MGG, 1977
It is known that DNA from phage T7 is not cleaved by restriction endonuclease EcoRI. However, DNA from phage T7am28 (gene 5) mutant (Studier, 1969) was found to be cleaved by the endonuclease at one site. The site is located at 0.46 fractional length from the left end of the molecule.
Yoshihide Tsujimoto, Hideyuki Ogawa
openaire   +3 more sources

Nuclease activity of 1,10-phenanthroline-copper ion: reaction with CGCGAATTCGCG and its complexes with netropsin and EcoRI.

Biochemistry, 1986
The self-complementing dodecamer 5'-CGCGAATTCGCG-3' and its complexes with the antibiotic netropsin and the restriction endonuclease EcoRI provide substrates of known three-dimensional structure to study the stereochemistry and mechanism of the ...
M. Kuwabara, C. Yoon, T. Goyne, T. Thederahn, D. Sigman   +4 more
semanticscholar   +1 more source

The EcoRI-DNA complex as a model for investigating protein-DNA interactions by atomic force microscopy.

Biochemistry, 2006
Atomic force microscopy (AFM) is a technique widely used to image protein-DNA complexes, and its application has now been extended to the measurements of protein-DNA binding constants and specificities. However, the spreading of the protein-DNA complexes
Isabelle Sorel, O. Piétrement, L. Hamon, S. Baconnais, E. Cam, D. Pastré   +5 more
semanticscholar   +1 more source

A frequent EcoRI polymorphism in the bcl-2 gene

Human Genetics, 1993
The frequency and Mendelian inheritance of an EcoRI polymorphism mapping within the bcl-2 locus was evaluated. The high frequency of the two identified alleles makes it a useful marker for genes located on the 18q21 region.
GHIA , PAOLO PROSPERO, Dianzani I, Serra A, Rosso C, Caligaris Cappio F.   +4 more
openaire   +3 more sources

Hydrostatic pressure reverses osmotic pressure effects on the specificity of EcoRI-DNA interactions.

Biochemistry, 1994
To characterize the role of water in protein-DNA interactions, we have studied the specificity of the EcoRI restriction endonuclease as a function of osmotic and hydrostatic pressure. The extent of cleavage by the enzyme at noncanonical ("star") sites is
C. R. Robinson, S. Sligar
semanticscholar   +1 more source

Footprinting of EcoRI endonuclease at high pressure

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1992
Hydroxyl radicals generated by irradiation with gamma rays have been used to footprint EcoRI endonuclease with single base pair resolution at pressures up to 144 MPa. At atmospheric pressure (0.1 MPa) a 10 base pair footprint was found. With increasing pressure three types of responses were observed: (1) bases distant from the recognition sequence ...
openaire   +3 more sources