Results 291 to 300 of about 227,914 (337)

Bending and flexibility of methylated and unmethylated EcoRI DNA

Journal of Molecular Biology, 2002
We used cyclization kinetics experiments and Monte Carlo simulations to determine a structural model for a DNA decamer containing the EcoRI restriction site. Our findings agree well with recent crystal and NMR structures of the EcoRI dodecamer, where an overall bend of seven degrees is distributed symmetrically over the molecule.
Dafna, Nathan, Donald M, Crothers
exaly   +3 more sources

EcoRI DNA methyltransferase-DNA interactions

Biochemistry, 1992
We present a novel strategy with synthetic hemimethylated DNA substrates containing uracil for thymine and inosine for guanosine replacements and EcoRI DNA methyltransferase to characterize the importance of major groove hydrophobic groups to the sequence-specific modification of DNA.
N O, Reich, M J, Danzitz
openaire   +2 more sources

Spectroscopic studies on the interaction between EcoRI and CdS QDs and conformation of EcoRI in EcoRI-CdS QDs bioconjugates

Physical Chemistry Chemical Physics, 2012
Water-soluble fluorescent quantum dots (QDs) have been widely used in biological and biomedical fields, and the interaction between QDs and proteins and the conformational structure of the protein in the bioconjugate has attracted increasing attention.
Yonghai, Song, Dan, Luo, Shuhong, Ye, Mei, Huang, Dandan, Zhong, Zhenzhong, Huang, Haoqing, Hou, Li, Wang   +7 more
openaire   +2 more sources

Oriented immobilization of restriction endonuclease ecori

Journal of Molecular Recognition, 1996
Two activated matrices have been developed to determine whether immobilization chemistry can be used to orient proteins on a support. Restriction endonuclease EcoRI from Escherichia coli RY13 (E.C.3.1.23.13) was used as a model in these studies. Thiol-activated Sephadex G-10 was used to couple the EcoRI endonuclease through its free sulfhydryl, while ...
M, Bircakova, M, Truksa, W H, Scouten
openaire   +2 more sources

EcoRI-sensitive mutation of T7 phage

Molecular and General Genetics MGG, 1977
It is known that DNA from phage T7 is not cleaved by restriction endonuclease EcoRI. However, DNA from phage T7am28 (gene 5) mutant (Studier, 1969) was found to be cleaved by the endonuclease at one site. The site is located at 0.46 fractional length from the left end of the molecule.
Y, Tsujimoto, H, Ogawa
openaire   +2 more sources

Temperature-sensitive mutants of the EcoRI endonuclease

Journal of Molecular Biology, 1997
The EcoRI endonuclease is an important recombinant DNA tool and a paradigm of sequence-specific DNA-protein interactions. We have isolated temperature-sensitive (TS) EcoRI endonuclease mutants (R56Q, G78D, P90S, V97I, R105K, M157I, C218Y, A235E, M255I, T261I and L263F) and characterized activity in vivo and in vitro.
R S, Muir, H, Flores, N D, Zinder, P, Model, X, Soberon, J, Heitman   +5 more
openaire   +2 more sources

RFLP Standardization Report for DQ Beta/EcoRI

1989
The DQ beta/EcoRI system identified 13 fragments in the core cell lines (Fig. 1). Locus assignments in this system (Table 1) showed fragments 1,2,4,5,6,10, & 13 to be DQB specific, the remaining fragments cross-hybridizing with both DRB and DPB (Table 3 in the DR Beta/EcoRI report).
S. Tonks, V. Groves, H. Betuel, J. L. Bidwell, M. L. Garavoy, R. Lechler, S. Naito, J. Bodmer, J. Trowsdale   +8 more
openaire   +1 more source

An electrochemiluminescence biosensor for endonuclease EcoRI detection

Biosensors and Bioelectronics, 2017
Endonucleases cleavage of DNA plays an important role in biological and medicinal chemistry. This work was going to develop a reliable and sensitive electrochemiluminescent (ECL) biosensor for detecting endonucleases by using gold nanoparticles graphene composite (GNPs-graphene) as a signal amplifier.
Yingjie, Li, Yuqin, Li, Yaoyu, Wu, Fushen, Lu, Yaowen, Chen, Wenhua, Gao   +5 more
openaire   +2 more sources

Kinetic mechanism of the EcoRI DNA methyltransferase

Biochemistry, 1991
We present a kinetic analysis of the EcoRI DNA N6-adenosine methyltransferase (Mtase). The enzyme catalyzes the S-adenosylmethionine (AdoMet)-dependent methylation of a short, synthetic 14 base pair DNA substrate and plasmid pBR322 DNA substrate with kcat/Km values of 0.51 X 10(8) and 4.1 X 10(8) s-1 M-1, respectively. The Mtase is thus one of the most
N O, Reich, N, Mashhoon
openaire   +2 more sources

EcoRI variant N199H has enhanced specific activity

Gene, 1996
The Asn199 residue of the EcoRI restriction endonuclease has been replaced with other amino acids to investigate whether it mediates nucleotide recognition or catalytic activity. Cassette mutagenesis gave variants of EcoRI: N199D, N199H, N199L, N199R, N199S and N199V. Their relative cleavage rates were found to be in the following order: N199H > EcoRI (
Kim, JJ, Min, Kyung-Tai, Kim, MH, Augh, SJ, Kim, BD, Lee, DS   +5 more
openaire   +3 more sources