Results 301 to 310 of about 165,447 (318)

Characterisation of plasmids coding for the restriction endonuclease EcoRI

Molecular and General Genetics MGG, 1976
The properties of two plasmids coding for the CcoRI restriction and modification enzymes are described. Both plasmids are non auto-transferring (NTP) but can be mobilised by transfer factors. Strains carrying NTP13 produce colicin E1 and the EcoRI enzymes.
Geraldine A. Willshaw, E. S. Anderson, Henry R. Smith, G. O. Humphreys   +3 more
openaire   +3 more sources

Molecular recognition mediated by bound water. A mechanism for star activity of the restriction endonuclease EcoRI.

Journal of Molecular Biology, 1993
Many restriction endonucleases such as EcoRI lose some specificity for their recognition sequence under certain buffer conditions. The cause of this disruption of accurate protein-DNA recognition has never been explained.
Clifford R. Robinson, S. Sligar
semanticscholar   +1 more source

Reversible inhibition of EcoRI with elevated pressure

Biochemical and Biophysical Research Communications, 1990
The endonuclease activity of EcoRI is completely inhibited at 200 MPa, 37 degrees C using the plasmid pBR 322 as a substrate. When assayed at 133 MPa approximately half the activity at atmospheric pressure was observed; from these data the standard molar volume change is estimated to be -80 cm3mol-1.
openaire   +3 more sources

Structure and recognition mechanism of EcoRI endonuclease

Trends in Biochemical Sciences, 1987
Abstract The structure of a complex between Eco RI endonuclease and a cognate oligonucleotide shows that sequence specificity is mediated by 12 protein-DNA hydrogen bonds. These interactions discriminate the Eco RI recognition site from all other sequences because any base substitution would rupture at least one of these hydrogen bonds.
Herbert W. Boyer, John Grable, Christin A. Frederick, Bi-Cheng Wang, Patricia J. Greene, Judith A. McClarin, John M. Rosenberg   +6 more
openaire   +2 more sources

Pausing of the restriction endonuclease EcoRI during linear diffusion on DNA.

Biochemistry, 1994
Linear diffusion is a mechanism to accelerate association rates beyond their three-dimensional diffusional limit. It is employed by the restriction endonuclease EcoRI as well as many other proteins interacting with specific DNA sequences to locate their ...
Albert Jeltsch, J. Alves, H. Wolfes, Guenter Maass, Alfred Pingoud   +4 more
semanticscholar   +1 more source

A cobalt derivative of the restriction enzyme EcoRI

Inorganica Chimica Acta, 1983
Although zinc is present in a range of enzymes that bind to DNA [1], the function of the Zn(II) dication either in the DNA binding process or in subsequent enzymic action is uncertain. Recently we have shown that the type II restriction endonuclease EcoRI [2] contains one equivalent tightly bound zinc per monomer and that the zinc ion is essential for ...
Jacqueline K. Barton, L.A. Basile
openaire   +2 more sources

The Role of Water in the EcoRI-DNA Binding

2004
In many respects, Type II restriction endonucleases are prototypical DNA-binding proteins. In order to avoid catastrophic consequences for the cell, however, these enzymes must be far more stringent in recogn ition of their target sequences and subsequent DNA cleavage than other specific sequence recognition proteins that regulate gene activity.
D. C. Rau, N. Sidorova
openaire   +2 more sources

RFLP Standardization Report for C4/EcoRI

1989
The panel of core cell lines cut with restriction enzyme MspI and hybridized with the C4 probe revealed a very simple pattern (Fig. 1). Two fragments were identified, of which only the first was polymorphic (1.37 kb) (Table 1).
M. Segall, John Trowsdale, C. Muller, S. Tonks, J. Bodmer, J. R. Regueiro, F. Otani, V. Groves   +7 more
openaire   +2 more sources

RFLP Standardization Report for 210H/EcoRI

1989
The 210H/MspI system identified six standard fragments in the core cell lines as shown in Table 1 and Figure 1. Much of the data received for this system was difficult to interpret because all the fragments were of low molecular weight and thus produced indistinct bands.
L. Schluender, M. Segall, H. Betuel, M. Garovoy   +3 more
openaire   +2 more sources

Structure of the DNA-EcoRI Endonuclease Recognition Complex

1987
The 3 A structure of the co-crystalline recognition complex between EcoRI endonuclease and the cognate oligonucleotide TCGCGAATTCGCG has been solved by the ISIR method using a platinum isomorphous derivative [1,2,3]. Refinement is in progress. The endonuclease-DNA recognition complex consists of a distorted double helix and a protein dimer composed of ...
Patricia J. Greene, Christin A. Frederick, John M. Rosenberg, Bi-Cheng Wang, John Grable, Judith A. McClarin, Herbert W. Boyer   +6 more
openaire   +2 more sources