Results 301 to 310 of about 227,914 (337)

Effect of high of pressure of EcoRI reactions

Journal of Molecular Recognition, 1994
AbstractThe effect of pressure on reactions of restriction endonucleases was investigated. No obvious irreversible (after) effect was observed for EcoRI, while a considerable irreversible inactivation was found for BamHI. Thus the EcoRI reactions against λ DNA, pBR322 and pBluescript were studied under high pressure and little effect was observed on ...
H, Kabata, A, Nomura, N, Shimamoto, S, Kunugi   +3 more
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Footprinting of EcoRI endonuclease at high pressure

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1992
Hydroxyl radicals generated by irradiation with gamma rays have been used to footprint EcoRI endonuclease with single base pair resolution at pressures up to 144 MPa. At atmospheric pressure (0.1 MPa) a 10 base pair footprint was found. With increasing pressure three types of responses were observed: (1) bases distant from the recognition sequence ...
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A new EcoRI polymorphism at the D21S13 locus

Human Genetics, 1990
The D21S13 locus has shown linkage to a gene for familial Alzheimer disease (FAD) on chromosome 21 (St. George-Hyslop et al. 1987). The limited informativeness of probes for this locus have hindered precise mapping of the FAD locus and analysis of nonallelic heterogeneity in FAD (Schellenberg et al. 1988; St. George-Hyslop et al. 1987).
S M, Pulst, J R, Korenberg, J, Greenwald, M, Carbone   +3 more
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Internal EcoRI-generated deletion in prophage Mu DNA

Biochemical and Biophysical Research Communications, 1980
Abstract The hybrid plasmid consisting of the plasmid pRP1.2 (derivative of RP4) genome and deleted prophage Mucts 62 genome which lost the central EcoRI fragment of DNA was constructed. The ability of deleted Mu phage to carry out E. coli chromosomal genes transposition was still retained.
S I, Alikhanian, A M, Kaplan, M A, Krupenko   +2 more
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Reversible inhibition of EcoRI with elevated pressure

Biochemical and Biophysical Research Communications, 1990
The endonuclease activity of EcoRI is completely inhibited at 200 MPa, 37 degrees C using the plasmid pBR 322 as a substrate. When assayed at 133 MPa approximately half the activity at atmospheric pressure was observed; from these data the standard molar volume change is estimated to be -80 cm3mol-1.
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EcoRI endonuclease cleavage map of bacteriophage P4-DNA

Virology, 1975
Abstract Satellite bacteriophage P4-DNA is cleaved by EcoRI endonuclease at three specific sites, 0.702, 0.737, and 0.985 fractional lengths as measured from the left end of the molecule. The EcoRI cleavage map was determined by electron microscopic length measurements and agarose-gel electrophoresis.
L, Goldstein, M, Thomas, R W, Davis
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A common EcoRI polymorphism at the ZFX locus

Human Molecular Genetics, 1992
The human pseudoautosomal region comprises a 2.6 megabase segment of the distal short arms of the X and Y chromosomes. Complete DNA sequence homology between the two sex chromosomes is found in this region, and is believed to be important in mediating X-Y pairing in male meiosis.
J, Ellison, C, Alles, L, Shapiro
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A rapid procedure for purification of EcoRI endonuclease

Biochemical and Biophysical Research Communications, 1977
Abstract A convenient and rapid procedure has been developed to purify restriction endonuclease Eco RI. The method involves sonication of cells at low ionic strength, precipitation of the endonuclease with Polymin P (a polyethyleneimine), elution of the enzyme from the Polymin P precipitate, ammonium sulfate precipitation and chromatography on ...
J, Sümegi, D, Breedveld, P, Hossenlopp, P, Chambon   +3 more
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Kinked DNA in crystalline complex with EcoRI endonuclease

Nature, 1984
The 3 A electron density map of a co-crystalline recognition complex between EcoRI endonuclease and the oligonucleotide TCGCGAATTCGCG reveals that a tight, complementary interface between the enzyme and the major groove of the DNA is the major determinant of sequence specificity.
C A, Frederick, J, Grable, M, Melia, C, Samudzi, L, Jen-Jacobson, B C, Wang, P, Greene, H W, Boyer, J M, Rosenberg   +8 more
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RFLP Standardization Report for C4/EcoRI

1989
The panel of core cell lines cut with restriction enzyme MspI and hybridized with the C4 probe revealed a very simple pattern (Fig. 1). Two fragments were identified, of which only the first was polymorphic (1.37 kb) (Table 1).
M. Segall, L. Schluender, H. Betuel, M. Garovoy   +3 more
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