Results 301 to 310 of about 225,565 (329)

A frequent EcoRI polymorphism in the bcl-2 gene

Human Genetics, 1993
The frequency and Mendelian inheritance of an EcoRI polymorphism mapping within the bcl-2 locus was evaluated. The high frequency of the two identified alleles makes it a useful marker for genes located on the 18q21 region.
GHIA , PAOLO PROSPERO, Dianzani I, Serra A, Rosso C, Caligaris Cappio F.   +4 more
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Footprinting of EcoRI endonuclease at high pressure

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1992
Hydroxyl radicals generated by irradiation with gamma rays have been used to footprint EcoRI endonuclease with single base pair resolution at pressures up to 144 MPa. At atmospheric pressure (0.1 MPa) a 10 base pair footprint was found. With increasing pressure three types of responses were observed: (1) bases distant from the recognition sequence ...
openaire   +3 more sources

Characterisation of plasmids coding for the restriction endonuclease EcoRI

Molecular and General Genetics MGG, 1976
The properties of two plasmids coding for the CcoRI restriction and modification enzymes are described. Both plasmids are non auto-transferring (NTP) but can be mobilised by transfer factors. Strains carrying NTP13 produce colicin E1 and the EcoRI enzymes.
Geraldine A. Willshaw, E. S. Anderson, Henry R. Smith, G. O. Humphreys   +3 more
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Reversible inhibition of EcoRI with elevated pressure

Biochemical and Biophysical Research Communications, 1990
The endonuclease activity of EcoRI is completely inhibited at 200 MPa, 37 degrees C using the plasmid pBR 322 as a substrate. When assayed at 133 MPa approximately half the activity at atmospheric pressure was observed; from these data the standard molar volume change is estimated to be -80 cm3mol-1.
openaire   +3 more sources

Structure and recognition mechanism of EcoRI endonuclease

Trends in Biochemical Sciences, 1987
Abstract The structure of a complex between Eco RI endonuclease and a cognate oligonucleotide shows that sequence specificity is mediated by 12 protein-DNA hydrogen bonds. These interactions discriminate the Eco RI recognition site from all other sequences because any base substitution would rupture at least one of these hydrogen bonds.
Herbert W. Boyer, John Grable, Christin A. Frederick, Bi-Cheng Wang, Patricia J. Greene, Judith A. McClarin, John M. Rosenberg   +6 more
openaire   +2 more sources

Kinked DNA in crystalline complex with EcoRI endonuclease

Nature, 1984
The 3 A electron density map of a co-crystalline recognition complex between EcoRI endonuclease and the oligonucleotide TCGCGAATTCGCG reveals that a tight, complementary interface between the enzyme and the major groove of the DNA is the major determinant of sequence specificity.
John Grable, Herbert W. Boyer, Christin A. Frederick, Michele Melia, Cleopas T. Samudzi, Bi-Cheng Wang, Linda Jen-Jacobson, Patricia J. Greene, John M. Rosenberg   +8 more
openaire   +3 more sources

A cobalt derivative of the restriction enzyme EcoRI

Inorganica Chimica Acta, 1983
Although zinc is present in a range of enzymes that bind to DNA [1], the function of the Zn(II) dication either in the DNA binding process or in subsequent enzymic action is uncertain. Recently we have shown that the type II restriction endonuclease EcoRI [2] contains one equivalent tightly bound zinc per monomer and that the zinc ion is essential for ...
Jacqueline K. Barton, L.A. Basile
openaire   +2 more sources

The Role of Water in the EcoRI-DNA Binding

2004
In many respects, Type II restriction endonucleases are prototypical DNA-binding proteins. In order to avoid catastrophic consequences for the cell, however, these enzymes must be far more stringent in recogn ition of their target sequences and subsequent DNA cleavage than other specific sequence recognition proteins that regulate gene activity.
D. C. Rau, N. Sidorova
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RFLP Standardization Report for C4/EcoRI

1989
The panel of core cell lines cut with restriction enzyme MspI and hybridized with the C4 probe revealed a very simple pattern (Fig. 1). Two fragments were identified, of which only the first was polymorphic (1.37 kb) (Table 1).
M. Segall, John Trowsdale, C. Muller, S. Tonks, J. Bodmer, J. R. Regueiro, F. Otani, V. Groves   +7 more
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RFLP Standardization Report for 210H/EcoRI

1989
The 210H/MspI system identified six standard fragments in the core cell lines as shown in Table 1 and Figure 1. Much of the data received for this system was difficult to interpret because all the fragments were of low molecular weight and thus produced indistinct bands.
L. Schluender, M. Segall, H. Betuel, M. Garovoy   +3 more
openaire   +2 more sources