A bacterial artificial chromosome-based physical map of Manihot esculenta ssp. flabellifolia [PDF]
Frontiers of Agricultural Science and Engineering, 2016 Cassava (Manihot esculenta) is known as the third most important food crop in the tropics and also used for industrial feedstock for biofuels. Two new bacterial artificial chromosome (BAC) libraries were constructed for W14 (M.
Yuhua FU,Zhiqiang XIA,Shujuan WANG,Xin CHEN,Cheng LU,Mingcheng LUO,Hongbin ZHANG,Wenquan WANG
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Optimization of the EcoRI*‐activity of EcoRI endonuclease [PDF]
FEBS Letters, 1978 sequences [5]. I have attempted to optimise the &OR*, activity through lowering of the ionic strength of the medium and by the addition of organic solvents. This study is of interest in elucidating the mechanism of interactjon between a nucleic acid sequence and a restriction endonuclease and furthermore for the evaluation of the useful range of ...
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Determinants of EcoRI endonuclease sequence discrimination. [PDF]
Proceedings of the National Academy of Sciences, 1989 The arginine at position 200 of EcoRI endonuclease is thought to make two hydrogen bonds to the guanine of the sequence GAATTC and thus be an important determinant of sequence discrimination. Arg-200 was replaced by each of the other 19 naturally occurring amino acids, and the mutant endonucleases were assessed for activities in vivo and in vitro.
M C, Needels +5 more
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Joining of Immunoglobulin Heavy Chain Gene Segments: Implications from a Chromosome with Evidence of Three D-JH Fusions [PDF]
, 1982 A chromosomal segment with a unique structure around the immunoglobulin heavy chain joining region (JH) has been molecularly cloned from an Abelson murine leukemia virus-transformed cell line.
Alt, Frederick W., Baltimore, David
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Ciência Rural, 1996 The chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro was isola ted from chloroplasts obtained by descontiuous sucrose gradient centrifugation. The restriction analysis with the enzymes HindIII, EcoRI and BamHI and their combination, allowed to ...
Sergio Echeverrigaray +2 more
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Targeted mutagenesis of the Sap47 gene of Drosophila: Flies lacking the synapse associated protein of 47 kDa are viable and fertile [PDF]
, 2004 BACKGROUND: Conserved proteins preferentially expressed in synaptic terminals of the nervous system are likely to play a significant role in brain function.
Becker, Sonja +4 more
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A 5'-region polymorphism modulates promoter activity of the tumor suppressor gene MFSD2A
Molecular Cancer, 2011 Background The MFSD2A gene maps within a linkage disequilibrium block containing the MYCL1-EcoRI polymorphism associated with prognosis and survival in lung cancer patients. Survival discrepancies between Asians and Caucasians point to ethnic differences
Kunitoh Hideo +6 more
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Organization and sequence of the gene encoding the human acrosin-trypsin inhibitor (HUSI-II) [PDF]
, 1993 A complete cDNA encoding the acrosin-trypsin inhibitor, HUSI-II, was used as a probe to isolate genomic clones from a human placenta library. Three clones which cover the entire HUSI-II gene were isolated and characterized.
Abelson +28 more
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Restriction enzymes use a 24 dimensional coding space to recognize 6 base long DNA sequences.
PLoS ONE, 2019 Restriction enzymes recognize and bind to specific sequences on invading bacteriophage DNA. Like a key in a lock, these proteins require many contacts to specify the correct DNA sequence.
Thomas D Schneider, Vishnu Jejjala
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Restriction Fragment Length Polymorphism Linkage Map for Arabidopsis thaliana [PDF]
, 1988 We have constructed a restriction fragment length polymorphism linkage map for the nuclear genome of the flowering plant Arabidopsis thaliana. The map, containing 90 randomly distributed molecular markers, is physically very dense; >50% of the genome is ...
Bowman, John L. +4 more
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