Results 31 to 40 of about 165,447 (318)

A bacterial artificial chromosome-based physical map of Manihot esculenta ssp. flabellifolia [PDF]

open access: yesFrontiers of Agricultural Science and Engineering, 2016
Cassava (Manihot esculenta) is known as the third most important food crop in the tropics and also used for industrial feedstock for biofuels. Two new bacterial artificial chromosome (BAC) libraries were constructed for W14 (M.
Yuhua FU,Zhiqiang XIA,Shujuan WANG,Xin CHEN,Cheng LU,Mingcheng LUO,Hongbin ZHANG,Wenquan WANG
doaj   +1 more source

Changes in solvation during DNA binding and cleavage are critical to altered specificity of the EcoRI endonuclease.

open access: yesProceedings of the National Academy of Sciences of the United States of America, 1998
Restriction endonucleases such as EcoRI bind and cleave DNA with great specificity and represent a paradigm for protein-DNA interactions and molecular recognition.
Clifford R. Robinson, S. Sligar
semanticscholar   +1 more source

Molecular epidemiology of a nosocomial outbreak due to Enterobacter cloacae and Enterobacter agglomerans in Campinas, São Paulo, Brazil Epidemiologia molecular de um surto de bacteriemia por Enterobacter cloacae e Enterobacter agglomerans ocorrido na região de Campinas, São Paulo, Brasil

open access: yesRevista do Instituto de Medicina Tropical de São Paulo, 2000
A total of 73 isolates (57 Enterobacter cloacae and 16 Enterobacter agglomerans), recovered during an outbreak of bacteremia in the Campinas area, São Paulo, Brazil, were studied.
Célia R. GONÇALVES   +10 more
doaj   +1 more source

Involvement of outside DNA sequences in the major kinetic path by which EcoRI endonuclease locates and leaves its recognition sequence.

open access: yesProceedings of the National Academy of Sciences of the United States of America, 1982
We have examined the kinetics of the interaction between endodeoxyribonuclease EcoRI (EC 3.1.23.13) and nine linear DNA fragments that range in size between 34 and 6,200 base pairs and contain the EcoRI site of plasmid pBR322 in a central location.
W. Jack, B. Terry, P. Modrich
semanticscholar   +1 more source

In vitro specificity of EcoRI DNA methyltransferase. [PDF]

open access: yesJournal of Biological Chemistry, 1992
The sequence selectivity of enzyme-DNA interactions was analyzed by comparing discrimination between synthetic oligonucleotides containing the canonical site GAATTC and altered DNA sequences with the EcoRI DNA methyltransferase. The specificities (kcat/KmDNA) are decreased from 5- to 23,000-fold relative to the unmodified site.
J Murphy   +3 more
openaire   +2 more sources

Marek's disease virus EcoRI-Q gene (meq) and a small RNA antisense to ICP4 are abundantly expressed in CD4+ cells and cells carrying a novel lymphoid marker, AV37, in Marek's disease lymphomas.

open access: yesJournal of General Virology, 1997
Mature lymphomas produced in Rhode Island Red (RIR) chickens infected with the RB1B strain of Marek's disease virus (MDV) were examined for the presence of viral DNA and RNA and expression of viral antigens.
N. Ross   +7 more
semanticscholar   +1 more source

Restriction enzymes use a 24 dimensional coding space to recognize 6 base long DNA sequences.

open access: yesPLoS ONE, 2019
Restriction enzymes recognize and bind to specific sequences on invading bacteriophage DNA. Like a key in a lock, these proteins require many contacts to specify the correct DNA sequence.
Thomas D Schneider, Vishnu Jejjala
doaj   +1 more source

Analysis of the genome of equine herpesvirus type 1: arrangement of cleavage sites for restriction endonucleases EcoRI, BglII and BamHI.

open access: yesJournal of General Virology, 1981
The genome of an Australian isolate of equine herpesvirus type 1 (equine abortion virus) has been analysed using the restriction endonucleases EcoRI, BglII and BamHI, and a physical map constructed.
Whalley Jm   +2 more
semanticscholar   +1 more source

A 5'-region polymorphism modulates promoter activity of the tumor suppressor gene MFSD2A

open access: yesMolecular Cancer, 2011
Background The MFSD2A gene maps within a linkage disequilibrium block containing the MYCL1-EcoRI polymorphism associated with prognosis and survival in lung cancer patients. Survival discrepancies between Asians and Caucasians point to ethnic differences
Kunitoh Hideo   +6 more
doaj   +1 more source

Differences in water release for the binding of EcoRI to specific and nonspecific DNA sequences.

open access: yesProceedings of the National Academy of Sciences of the United States of America, 1996
The free energy difference between complexes of the restriction nuclease EcoRI with nonspecific DNA and with the enzyme's recognition sequence is linearly dependent on the water chemical potential of the solution, set using several very different solutes,
N. Sidorova, Donald C. RAUt
semanticscholar   +1 more source

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