A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells [PDF]
, 2013 Growth and differentiation of multicellular systems is orchestrated by spatially restricted gene expression programs in specialized subpopulations. The targeted manipulation of such processes by synthetic tools with high-spatiotemporal resolution could ...
Busacker, Moritz +5 more
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Dynamical Consequences of Bandpass Feedback Loops in a Bacterial Phosphorelay [PDF]
, 2011 Under conditions of nutrient limitation, Bacillus subtilis cells terminally differentiate into a dormant spore state. Progression to sporulation is controlled by a genetic circuit consisting of a phosphorelay embedded in multiple transcriptional feedback
Elowitz, Michael B. +2 more
core +8 more sources
Ciência Rural, 1996 The chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro was isola ted from chloroplasts obtained by descontiuous sucrose gradient centrifugation. The restriction analysis with the enzymes HindIII, EcoRI and BamHI and their combination, allowed to ...
Sergio Echeverrigaray +2 more
doaj +1 more source
In vitro specificity of EcoRI DNA methyltransferase. [PDF]
Journal of Biological Chemistry, 1992 The sequence selectivity of enzyme-DNA interactions was analyzed by comparing discrimination between synthetic oligonucleotides containing the canonical site GAATTC and altered DNA sequences with the EcoRI DNA methyltransferase. The specificities (kcat/KmDNA) are decreased from 5- to 23,000-fold relative to the unmodified site.
J Murphy +3 more
openaire +2 more sources
Restriction enzymes use a 24 dimensional coding space to recognize 6 base long DNA sequences.
PLoS ONE, 2019 Restriction enzymes recognize and bind to specific sequences on invading bacteriophage DNA. Like a key in a lock, these proteins require many contacts to specify the correct DNA sequence.
Thomas D Schneider, Vishnu Jejjala
doaj +1 more source
A 5'-region polymorphism modulates promoter activity of the tumor suppressor gene MFSD2A
Molecular Cancer, 2011 Background The MFSD2A gene maps within a linkage disequilibrium block containing the MYCL1-EcoRI polymorphism associated with prognosis and survival in lung cancer patients. Survival discrepancies between Asians and Caucasians point to ethnic differences
Kunitoh Hideo +6 more
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Joining of Immunoglobulin Heavy Chain Gene Segments: Implications from a Chromosome with Evidence of Three D-JH Fusions [PDF]
, 1982 A chromosomal segment with a unique structure around the immunoglobulin heavy chain joining region (JH) has been molecularly cloned from an Abelson murine leukemia virus-transformed cell line.
Alt, Frederick W., Baltimore, David
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Targeted mutagenesis of the Sap47 gene of Drosophila: Flies lacking the synapse associated protein of 47 kDa are viable and fertile [PDF]
, 2004 BACKGROUND: Conserved proteins preferentially expressed in synaptic terminals of the nervous system are likely to play a significant role in brain function.
Becker, Sonja +4 more
core +3 more sources
Specificity of substrate recognition by the EcoRI restriction endonuclease. [PDF]
Proceedings of the National Academy of Sciences, 1975 The substrate specificity of the EcoRI restriction endonuclease can be varied in vitro by changing the pH and the ionic environment of the reaction. Phosphodiester bond cleavage occurs at a DNA hexanucleotide sequence d(N-G-A-A-T-T-C-N)/d(N-C-T-T-A-A-G-N) when the ionic strength is high, 100 mM Tris-HCl, 50 mM NaCl, 5 mM MgCl2, and the pH is ...
Herbert W. Boyer +5 more
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Method for Cloning Restriction Fragments Containing the Termini of BAC Inserts
BioTechniques, 2000 We have developed a method to isolate the termini of BAC clones. The method is based on the two unique NotI sites located approximately 300 bp on either side of the EcoRI cloning site of the BAC vector pECSBAC4. Our strategy includes the following steps:
Tammy R. Plyler, C. Eduardo Vallejos
doaj +1 more source